Objective To explore the possible mechanism of Sugemule-3 Decoction（苏格木勒-3汤） in the treatment of heart failure. Methods HL-1 cardiomyocytes were inoculated into 96-well plates at 1×10

5

cells per well

and they were grouped after 24 h of routine culture. In blank control group

there were normal cardiomyocytes without any treatment. Model group was added with 100μl isoproterenol per well with a final concentration of 25μg/ml to process for 24 h. After being added with 100μl isoproterenol per well with a final concentration of 25μg/ml to process for 24 h

Sugemule-3 Decoction groups of high

medium and low doses were added with 75

50

and 25μg/ml water extract of Sugemule-3 Decoction

respectively

to process for 24 h

with 100μl per well. There were 5 double wells in each group. MTT method was used to detect the survival rate of HL-1 cardiomyocytes in each group. Calcium ion concentration was detected by fluorescence microscope. Western blotting method was used to detect protein expression of sarcoplasmic reticulum calcium ATPase 2 a（SERCA2 a） in HL-1 cardiomyocytes. Results Compared with the blank control group

the survival rate of HL-1 cardiomyocytes and the expression of SERCA2 a protein in the model group were decreased（P

<

0.01）. Compared with the model group

the survival rates of HL-1 cardiomyocytes in the high and medium dose groups of Sugemule-3 Decoction were increased

and the expressions of SERCA2 a protein in HL-1 cardiomyocytes of the high

medium and low dose groups of Sugemule-3 Decoction were increased（P

<

0.01）. Fluorescence microscope showed that the fluorescence intensity of calcium ion concentration in HL-1 cardiomyocytes of the model group was increased; compared with the model group

the fluorescence intensities of calcium ion concentration in HL-1 cardiomyocytes in the medium and high dose groups of Sugemule-3 Decoction were decreased. Conclusion Mongolian medicine Sugemule-3 Decoction can inhibit calcium overload of HL-1 cardiomyocytes

and its mechanism may be related to increasing SERCA2 a protein expression in cardiomyocytes.