Objective To explore possible mechanism of Xiangsha Liujunzi Decoction（香砂六君子汤

XSLJZ） in treating functional dyspepsia（FD）. Methods A total of 36 male Sprague-Dawley（SD） rats were randomly divided into blank group

model group and XSLJZ group

with 12 rats in each group. The model group and the XSLJZ group were administrated with iodoacetamide by gavage combined with tail clamping to establish FD rat models. The XSLJZ group was given 1 ml/100 g（concentration of 0. 7875 g/ml） XSLJZ by gavage

while the blank group and the model group were given equal volume of normal saline by gavage

once a day. After 7 days

gastric tissue morphology was observed by HE staining

and rat’s gastric sensitivity was detected. Transient receptor potential vanilloid type 1（TRPV1） mRNA content in duodenum was detected by q PCR

and 5-hydroxytryptamine（5-HT） content in duodenum was detected by ELISA. Immunofluorescent double labelled staining was applied to detect expressions and distributions of TRPV1 protein and 5-HT in duodenum. Results The gastric histomorphology in the 3 groups was normal. Compared with the blank group

the gastric sensitivity was significantly increased in the model group（P <0. 01）. Compared with the model group

the gastric sensitivity was decreased（P < 0. 01）. The expression of TRPV1 mRNA and the content of 5-HT in duodenum in the model group were significantly increased compared with the blank group（P < 0. 01）. Compared with the model group

the expression of TRPV1 mRNA and the content of 5-HT in duodenum in the XSLJZ group were significantly decreased（P < 0. 01）. The TRPVl protein were expressed in the duodenum along with 5-HT

and the expressions of TRPV1 protein and 5-HT in the model group were more than those in the blank group

while the expression in the XSLJZ group were decreased compared with those in the model group.Conclusion XSLJZ can reduce expression levels of duodenal TRPV1 and 5-HT in FD rats to relieve gastric hypersensitivity

which may be one of its mechanisms in treating FD.