Objective To explore methylated changes of adipose tissue metabolism-related gene promoters in obesity of phlegm-dampness syndrome model rats and the possible intervention mechanism of Wendan Decoction（温胆汤）. Methods Rat models of obesity of phlegm-dampness syndrome were established by feeding a high-fat diet for 8 weeks

and the successful modeling rats were divided into Wendan Decoction group and model group according to random number table method

with 10 rats in each group. Another 10 rats were set as normal group. After the modeling

rats in all groups were fed with common feed. At the same time

the Wendan Decoction group was given 15 g/（kg·d） Wendan Decoction by gavage

and the model group and normal group were given the same volume of distilled water

treating for 4 weeks. Methylated DNA immunoprecipitation sequencing（MeDIP-seq） method was used to screen promoter methylation differential genes and metabolism-related genes in rat abdominal adipose tissue of each group

and the results of promoter methylation detection were verified by pyrosequencing. Results In the metabolism-related genes in obesity of phlegm-dampness syndrome

methylation degrees of 19 promoters were decreased and the methylation degrees of 36 promoters were increased. After the intervention of Wendan Decoction in obesity of phlegm-dampness syndrome

the methylation degrees of 14 methylated differential genes related to metabolism were decreased

and the methylation degrees of 38 methylated differential genes were increased

in which there were 7 differential genes of promoter methylation related to obesity of phlegm-dampness syndrome

namely B4 galt7

Elof1

Iws1

Kynu

Sc5 d

Mutyh and Tgif2. Conclusion There are changes in the methylated status of adipose tissue metabolism-related gene promoters in obesity of phlegm-dampness syndrome model rats

and Wendan Decoction has a certain regulatory effect on the methylation of metabolism-related gene promoters.