Objective To observe the effects of drug-containing serum of Huayu Pills（化瘀丸） on doxorubicin content in Lewis cells of lung cancer. Methods Screen the concentration of doxorubicin in Lewis cells by MTT method. The stably growing Lewis cells were added drug-containing serum of Huayu Pills medicated medium and blank medium

and after 48 hours of cultivation

doxorubicin solution of final concentration was added. The distribution of doxorubicin in the cells was observed under a microscope

and the multi-functional microplate reader quantitatively determined the change in the fluorescence signal intensity of doxorubicin in the cells. Effects of the drug-containing serum of Huayu Pills on the sensitivity of doxorubicin chemotherapy was determined by MTT method. Results The concentration of doxorubicin is 0.1μmol/L. Doxorubicin is localized in the nucleus. Compared with the blank serum group

the drug-containing serum group of Huayu Pills promoted the transfer of doxorubicin from Lewis lung cancer cells from extracellular to intracellular. The fluorescence intensity value of Lewis cells in the serum-containing group of Huayu Pills was 78.43 ± 1.70

higher than 61.33 ± 1.71 in the blank serum control group（P<0.001）. At 0.20

0.25

and 0.30 mol/L doxorubicin administration concentration

the cytostatic rate of drug-containing serum of Huayu Pills group was significantly higher than that of blank serum group（P<0.05）

and 0.30μmol/L doxorubicin administration concentration got the highest cell suppression rate. Conclusion Drug-containing serum of Huayu Pills can increase doxorubicin content in Lewis cells and has the synergistic effect with chemotherapy.