Objective To explore the possible mechanism of Jianpi Huatan Formula（健脾化痰方） in the treatment of hyperlipidemia with spleen deficiency and phlegm turbid syndrome. Methods A total of 15 ordinary Guangxi Bama minipigs were randomly divided into a normal group

a model group and a treatment group

with 5 in each group. The normal group was fed with basic feed

the model group and the treatment group were fed with high-fat feed for 24 weeks to establish a small pig model of hyperlipidemia with spleen deficiency and phlegm turbidity syndrome. The treatment group was given Jianpi Huatan Formula Granules（健脾化痰方颗粒剂） at the same time given high-fat feed. The granules were dissolved in 100 ml of normal saline and evenly mixed into the feed. One dose per day for 24 weeks. At 0 week and 24 weeks

fasting auricular venous blood of the minipigs was collected to detect the levels of nitric oxide（NO）

endothelial nitric oxide synthase（eNOS）

inducible nitric oxide synthase（iNOS）

total nitric oxide synthase（TNOs）

serum endothelin 1（ET-1） and intercellular adhesion molecule 1（ICAM-1）. After 24 weeks

the endothelial function index and ET-1

ICAM-1 level in endothelium homogenate of coronary artery was measured. Results At 24 weeks

compared with the control group

the levels of tNOS

iNOS

ET-1

ICAM-1 were increased

and the levels of eNOS

NO were decreased in the model group（P<0.05）. Compared with the model group

the levels of tNOS

iNOS

ET-1

ICAM-1 in minipig serum and coronary artery endothelial tissue homogenate were decreased

and the levels of eNOS and NO were increased（P<0.05）. Conclusion Jianpi Huatan Formula（健脾化痰方） can effectively improve the function of endothelium

which may be one of the mechanisms of its treatment of hyperlipidemia with spleen deficiency and phlegm turbid syndrome.