Objective To explore the anti-tumor effects and possible mechanism of Herba Hedyotis

Herba Scutellariae Barbatae and their drug pair combination. Methods Human pancreatic cancer Panc28 cells were inoculated subcutaneously in the armpits of BALB/c female mice. When the subcutaneous transplanted tumor grew to about 100 mm

3

the drug was administered. The cytoxan for injection（50 mg/kg） was used as a positive control. Herba Hedyotis

Herba Scutellariae Barbatae

Herba Hedyotis with Herba Scutellariae Barbatae were injected intraperitoneally into the concentrated solution at 200 mg/kg

once every other day for 8 times

and the tumor volume and body weight of mice were measured every other day. Panc28 and human liver cancer Bel7402 cells in logarithmic growth phase were cultured and was divided into a control group

a Herba Hedyotis group

a Herba Scutellariae Barbatae group

and a drug pair group. All group but the control group were added with the concentrations of 400

200

100

50 and 25 μg/ml of Herba Hedyotis concentrate

Herba Scutellariae Barbatae Concentrate

and Herba Hedyotis with Herba Scutellariae Barbatae respectively concentrate for 48 hours to detect cells inhibition rate under different concentrations and calculate half the inhibitory concentration(IC

50

). We observed relative content of glucose and lactate in supernate after 24 h treated with the final concentration 50μg/ml Herba Hedyotis single decoction concentrate

Herba Scutellariae Barbatae single decoction concentrate

and Herba Hedyotis

Herba Scutellariae Barbatae mixed decoction concentrate and the expression of pyruvate kinase 1（PKM1） and pyruvate kinase 2（PKM2） proteins after 48 hours. Results The transplanted tumor volume of the drug pair group and the positive control group of mice was smaller than that of Herba Hedyotis group

Herba Scutellariae Barbatae group（P

<

0.05）. All drugs had no effects on the boty weight of the mice（P

>

0.05）. Compared with the Herba Hedyotis group and Herba Scutellariae Barbatae group

the IC

50

of Panc28 cells and Bel7402 cells in the drug-pair group decreased

and the relative glucose uptake and lactate levels were significantly reduced（P

<

0.05）

while PKM1 protein expression increased in Panc28 cells and Bel7402 cells

and PKM2 protein expression decreased（P

<

0.05）. Conclusion Herba Hedyotis

and Herba Scutellariae Barbatae drug pair could inhibit tumor growth

and its mechanism may be related to the inhibition of tumor cell glycolysis.