Objective To study the effects of "Zusanli（ST 36）" and "Liangmen（ST 21）" on pathology of gastric mucosa and metabolite profile of cerebral cortex in rats with stomach heat syndrome

and stomach cold syndrome. Methods A total of 42 male SD rats were randomly divided into a blank group（n = 10）

a stomach heat model group（n = 16）

and a stomach cold model group（n = 16）. In the model of stomach heat syndrome

the rat model of stomach-heat syndrome was replicated by gavage with chili liquid and ethanol

and the rat model of stomach-cold syndrome was replicated by gavage with ice water and sodium hydroxide（NaOH）. After modeling

6 rats from each model group were randomly selected as moxibustion stomach heat group and moxibustion stomach cold group. The blank group

stomach heat model group and stomach cold model group did not intervene

and the rats in moxibustion stomach heat group and moxibustion stomach cold group underwent mild moxibustion intervention at "Zusanli（ST 36）" and "Liangmen（ST 21）" for 20 min

once daily for 7 days. HE staining was used to observe the histopathological changes of gastric mucosa; metabolomic examination of the cerebral cortex was performed to analyze the data and metabolic pathways. Results The gastric mucosal tissues in the stomach heat model group and the stomach heat model group conformed to the pathological manifestations of the stomach heat syndrome and the stomach cold syndrome. The pathology of the gastric mucosa in the moxibustion stomach heat group and the moxibustion stomach cold group was improved compared with the model group. Metabolomics found that 12 cerebral cortex metabolites were closely related to the stomach heat syndrome and 11 were closely related to the stomach cold syndrome. Moxibustion mainly regulates the metabolism of taurine and hypotaurine

niacinamide and phosphatidylinositol metabolism

and metabolism of alanine aspartic acid and glutamic acid in the cerebral cortex. And it can repair the gastric mucosal damage of stomach heat syndrome. Through the biosynthesis of valine

leucine and isoleucine

phosphoinositide metabolism can repair the gastric mucosal damage in patients with stomach cold syndrome. Conclusion The main effects mechanism of moxibustion on the repair of gastric mucosa in stomach heat syndrome involves the regulation of antagonism against oxidative stress

amino acid metabolism and maintenance of cell morphology. The main effect mechanism of gastric mucosal repair on stomach cold syndrome involves the amino acid in vivo metabolism

and maintaining cell morphology regulation.