Objective To explore the effects and mechanism of Radix Astragali seu Hedysa and Rhizoma Curcumae synergy on the adhesion and migration ability of mouse colon cancer cells CT26 in vitro. Methods CT26 cells were cultured in vitro

and a blank control group was set up. After treatment with the concentrations of 0.00625

0.0125

0.025

0.05

0.1

0.2 g/ml of Radix Astragali seu Hedysa and Rhizoma Curcumae respectively for 24 hours and 48 hours

the CCK-8 method was used to detect the effects of Radix Astragali seu Hedysa and Rhizoma Curcumae on the survival rate of CT26 cells in order to select a suitable concentration for administration. The scratch test was used to detect the effects of the compatibility of Radix Astragali seu Hedysa and Rhizoma Curcumae synergy on CT26 cell migration

and the cell adhesion test was used to detect the effects of the compatibility of Radix Astragali seu Hedysa and Rhizoma Curcumae synergy on the adhesion between CT26 cells and cells and extracellular matrix. After 24 hours of drug treatment

Western blot and RT-PCR were used to detect the adhesion-related factors E-cadherin

anti-cancer protein NO.1（KAI1）

tumor suppressor gene tension protein homologous NO.10 chromosome-deleted phosphatase gene（PTEN）

matrix proteinase-inducible factor（CD147）

hypoxia-inducible factor-1α（HIF-1α）

matrix metalloproteinase 9（MMP-9） protein and mRNA expression in CT26 cells. Results The concentrations of 0.0125

0.025 and 0.05 g/ml were selected for subsequent experiments. Compared with the blank control group

the migration rates of CT26 cells at 0.025

0.05 g/ml concentration of Radix Astragali seu Hedysa and Rhizoma Curcumae group at 24 h and 0.0125

0.025

0.05 g/ml concentration at 48 hour were significantly reduced. The migration rate of CT26 cells at 0.025 and 0.05 g/ml concentrations at 24 hours and 48 hours was significantly reduced; the migration rate of CT26 cells at 0.05 g/ml concentration at 24 hours and 48 hours was significantly reduced compared to the concentration at 0.025 g/ml. Except for the 0.05 g/ml concentration

the migration rate of CT26 cells in all groups at 48 hour was significantly higher than that at 24 hour within group（P<0.05 or P<0.01）. Compared with the blank control group

at the concentration of 0.0125

0.025

0.05 g/ml

the adhesion rate of CT26 cell with extracellular matrix in the Radix Astragali seu Hedysa and Rhizoma Curcumae groups were significantly decreased; compared with the concentration of 0.0125 g/ml

the concentration of 0.025

0.05 g/ml

the adhesion rate of CT26 cell with cells increased significantly

and the 0.05 g/ml concentration the extracellular matrix adhesion rate decreased significantly; compared with the 0.025 g/ml concentration

the 0.05 g/ml concentration cell adhesion rate increased significantly（P<0.05 or P<0.01）. Compared with the blank control group

the expression levels of E-cadherin

KAI1

PTEN protein and mRNA in CT26 cells of 0.025 and 0.05 g/ml concentration in Radix Astragali seu Hedysa and Rhizoma Curcumae group were significantly increased

and the expression of CD147 protein was significantly reduced; compared with the concentration of 0.0125 g/ml

under 0.025 g/ml concentration E-cadherin protein and mRNA expression increased significantly

under 0.05 g/ml concentration E-cadherin

KAI1

PTEN protein and mRNA expression increased significantly

CD147 protein and mRNA expression decreased; compared with 0.025 g/ml concentration

under 0.05 g/ml concentration KAI1

PTEN protein and mRNA expression increased significantly

CD147 protein and mRNA expression decreased（P<0.05 or P<0.01）. Conclusion The synergy of Radix Astragali seu Hedysa and Rhizoma Curcumae can enhance the adhesion between CT26 cells

weaken the adhesion ability with extracellular matrix

and inhibit its migration ability. The mechanism may be related to the promotion of the expression of homologous adhesion-related factors E-cadherin

PTEN

KAI1

and inhibition of heterologous adhesion-related factors CD147

MMP-9

and HIF-1α.