Effects of Modified Simiao Decoction （加味四妙方） on the Expression of NLRP3 mRNA, IL-1β, TNF-α in Ankle Joints Tissue of Hyperuricemia Model Rats Combined with Acute Gouty Arthritis

LIU Yafei; CHEN Zhe; TU Shenghao

doi:10.13288/j.11-2166/r.2020.14.017

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Effects of Modified Simiao Decoction （加味四妙方） on the Expression of NLRP3 mRNA, IL-1β, TNF-α in Ankle Joints Tissue of Hyperuricemia Model Rats Combined with Acute Gouty Arthritis

更新时间：2021-11-10

- Effects of Modified Simiao Decoction （加味四妙方） on the Expression of NLRP3 mRNA, IL-1β, TNF-α in Ankle Joints Tissue of Hyperuricemia Model Rats Combined with Acute Gouty Arthritis
- Journal of Traditional Chinese Medicine Vol. 61, Issue 14, Pages: 1268-1272(2020)
- 作者机构：
  
  1. 郑州大学第一附属医院
  2. 华中科技大学同济医学院附属同济医院
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2020.14.017
  CLC： R285.5
- Published：2020
- 稿件说明：
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[1]刘亚飞,陈哲,涂胜豪.加味四妙方对高尿酸血症合并急性痛风性关节炎模型大鼠踝关节组织NLRP3 mRNA及IL-1β、TNF-α表达的影响[J].中医杂志,2020,61(14):1268-1272.

LIU Yafei, CHEN Zhe, TU Shenghao. Effects of Modified Simiao Decoction （加味四妙方） on the Expression of NLRP3 mRNA, IL-1β, TNF-α in Ankle Joints Tissue of Hyperuricemia Model Rats Combined with Acute Gouty Arthritis[J]. Journal of Traditional Chinese Medicine2020, 61(14): 1268-1272.
[1]刘亚飞,陈哲,涂胜豪.加味四妙方对高尿酸血症合并急性痛风性关节炎模型大鼠踝关节组织NLRP3 mRNA及IL-1β、TNF-α表达的影响[J].中医杂志,2020,61(14):1268-1272. DOI： 10.13288/j.11-2166/r.2020.14.017.

LIU Yafei, CHEN Zhe, TU Shenghao. Effects of Modified Simiao Decoction （加味四妙方） on the Expression of NLRP3 mRNA, IL-1β, TNF-α in Ankle Joints Tissue of Hyperuricemia Model Rats Combined with Acute Gouty Arthritis[J]. Journal of Traditional Chinese Medicine2020, 61(14): 1268-1272. DOI： 10.13288/j.11-2166/r.2020.14.017.

摘要

目的探讨加味四妙方治疗高尿酸血症（HUA）合并急性痛风性关节炎（GA）的可能作用机制。方法 48只Wistar大鼠随机分为空白组

模型组

加味四妙方低、中、高剂量组

西药组

每组8只。各组造模与灌胃同时进行。加味四妙方低、中、高剂量组分别给予加味四妙方16.8、33.6、67.2g/（kg·d）灌胃

西药组给予别嘌醇0.09g/kg+秋水仙碱0.05mg/100g灌胃

空白组及模型组给予10ml/kg蒸馏水灌胃。各组连续给药7d。除空白组外

其余各组腹腔注射3%氧嗪酸钾溶液建立HUA模型

再于大鼠右踝关节腔内注射尿酸钠溶液诱导GA模型。观察大鼠踝关节周径变化

检测大鼠血尿酸（SUA）、C反应蛋白（CRP）水平

大鼠踝关节组织核苷酸结合寡聚化结构域样受体蛋白3（NLRP3）mRNA、白细胞介素1β（IL-1β）和肿瘤坏死因子α（TNF-α）表达。结果与空白组相比

模型组大鼠踝关节周径明显增加

血清SUA、CRP水平升高

踝关节组织NLRP3 mRNA及IL-1β、TNF-α表达显著升高（P<0.01）。与模型组相比

加味四妙方组中、高剂量组和西药组大鼠踝关节周径缩小

血清SUA、CRP水平降低

踝关节组织NLRP3 mRNA、IL-1β、TNF-α表达均降低（P<0.05）。与加味四妙方高剂量组比较

西药组血清CRP水平、踝关节组织NLRP3 mRNA表达显著降低（P<0.05）。结论加味四妙方可缓解HUA合并GA关节肿胀

降低血清SUA水平

其作用机制可能与调控NLRP3炎性体及下游炎症因子IL-1β和TNF-α的分泌有关。

Abstract

Objective To explore the possible mechanism of Modified Simiao Decoction（加味四妙方） on hyperuricemia（HUA） and acute gouty arthritis（GA）. Methods A total of 48 male Wistar rats were randomly divided into a control group

a model group

a Modified Simiao Decoction low dose group

medium dose group

high dose group

and a western medicine group with 8 rats in each group. The low

medium

high dose group of Modified Simiao Decoction were respectively given 16.8、33.6、67.2 g/（kg·d）by gavage

and the western medicine group was given 0.09 g/kg allopurinol plus colchicine 0.05 mg/100 g. The control group and model group were given intragastric administration with 10 ml/kg distilled water. All groups were continuously administered for 7 days. Except for the control group

rats were injected intraperitoneally with 3% potassium oxazidate solution to establish the HUA model

and then the sodium urate solution was injected into the right ankle joint cavity of rats to induce the GA model. The changes of rat ankle joint diameter

the levels of blood uric acid（SUA） and C-reactive protein（CRP）

and the nucleotide binding oligomerization domain-like receptor protein 3（NLRP3） mRNA of ankle joint tissue were detected. Interleukin 1β（IL-1β） and tumor necrosis factor α（TNF-α） expression were observed. Results Compared with the control group

ankle circumference

level of serum SUA and CRP

and expression of NLRP3 mRNA

IL-1β and TNF-α in ankle tissue were significantly increased in the model group（P<0.01）. Compared with the model group

the ankle joints circumference

serum SUA and CRP levels

expression of NLRP3 mRNA

IL-1β

and TNF-α in the ankle tissue were significantly decreased in the medium and high dose group of Modified Simiao Decoction

and the western medicine group（P<0.05）. Compared with Modified Simiao Decoction high dose group

the serum CRP level and the expression of NLRP3 mRNA in ankle joint tissue in the western medicine group were significantly reduced（P<0.05）. Conclusion Modified Simiao Decoction can relieve joint swelling of HUA combined with GA and reduce level of SUA. Its mechanism may be related to the regulation of the secretion of NLRP3 inflammasome and downstream inflammatory factors IL-1β and TNF-α.

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Related Institution

The First Hospital of Hunan University of Chinese Medicine

Experimental Research Center，China Academy of Chinese Medical Sciences

Institute of Xin'an Medicine and Modernization of Traditional Chinese Medicine，Institute of Health，Hefei Comprehensive National Science Center

Anhui Provincial Key Laboratory of Meridian and Zang-Fu Organs Interrelationships

The First Affiliated Hospital of Anhui University of Chinese Medicine

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