Objective To establish an animal model of yin-deficiency syndrome and to establish a systematic evaluation method of yin-deficiency syndrome. Methods C57 BLKS/J mice were randomly divided into normal group and yin-deficiency syndrome group

with nine rats in each group. Yin-deficiency syndrome group was administrated with 0.1 ml/10 g body weight yin-damaging drugs at a concentration of 1 g/ml daily for 4 weeks

and the normal group was given the same volume of ultra-pure water. Open field test was carried out to assess the central grid-crossing times

the percentage of the central grid-staying time

the total distance and speed of movement in the open field. The body temperature of mice was dynamically monitored

and the changes of water intake

saliva flow rate

fecal water content

skin water content

body mass

urobilinogen and urinary bilirubin

food intake and tensile force were measured and compared between groups. Results In the open field test

the central grid-crossing times

the percentage of the central grid-staying time

the total distance and speed of movement in the open field were significantly higher in the yin-deficiency syndrome group than those in the normal group（P<0.05 or P<0.01）. After 4 weeks of administration of yin-damaging drugs

it was found that the claw center temperature of the upper left limb and the lower right limb increased from 8:30 a.m. to 15:00 p.m.

and all four limbs had higher temperature at 20:00 p.m. compared to those at 8:30 a.m.（P<0.05 or P<0.01）. Compared to the normal group

the yin-deficiency syndrome group had lower saliva flow rate

skin water content of the limbs

body mass and food intake

higher paw temperature of the four limbs and water intake（P<0.05 or P<0.01）

as well as higher weak-positive rate of urobilinogen and positive rate of urinary bilirubin at the 4 th week（P<0.01）. Conclusion The method of consuming yin-fluid can be used to successfully establish an animal model of yin-deficiency syndrome. The evaluation method based on the typical manifestations of yin-deficiency syndrome

such as five vexing heat in the five centers（chest

palms and soles）

tidal fever

dry mouth

dry skin

dry stool

emaciated body and red urine

has been formulated.