Objective To explore the anti-tumor effect of Huagai Powder（华盖散） and its possible mechanism. Methods H1688 and A549 cells in logarithmic growth and good condition were seeded in a 96-well plate at a density of 5×10

3

; different concentrations of Huagai Powder at 125

250

500 and 1000μg/ml were given while a blank group was set

with five compound holes in each group. After incubating the lung cancer cells at 37℃ and with 5% CO

2

for 24

48

and 72 hours

the effects of different concentrations of Huagai Powder on cell proliferation were assessed

as well as and the effect on lung cancer cell apoptosis after 24 hours. Tandem Mass Tag（TMT） labeled quantitative protein technology combined with liquid chromatography tandem mass spectrometry was used to explore the differentially expressed proteins in the lung cancer cells after 24 hours′ intervention of Huagai Powder on H1688 cells

and the differential proteins screened by bioinformatics analysis were verified by Western blotting. Results Compared to the blank group

Huagai Powder at different concentrations administered for different time could all inhibit the proliferation rate of H1688 and A549 cells and increase the apoptosis rate

and the effect was dose-dependent（P

<

0.05）. Proteomics analysis showed that Huagai Powder could induce the differential expression of 112 proteins

including 58 up-regulated proteins and 54 down-regulated proteins. The UHRF1

CRYAB

ING3 and PAWR proteins related to cell proliferation and apoptosis were selected for Western blotting verification

and the results showed that the expression of ING3 and PAWR proteins was down-regulated while the expression of UHRF1 and CRYAB proteins was up-regulated in Huagai Powder group（P

<

0.05）. Conclusion Huagai Powder can inhibit the proliferation and induce apoptosis of H1688 and A549 lung cancer cells

and UHRF1

CRYAB

ING3 and PAWR may be biomarkers for the treatment of lung cancer.