Effect of <italic style="font-style: italic">Liushen Pill </italic>（六神丸） on the Constitution and Diversity of Upper Respiratory Tract Flora in Healthy People

Xiaolong XU; Yishan CHEN; Yunjing BAI; Yuhong GUO; Qingquan LIU

doi:10.13288/j.11-2166/r.2021.23.008

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Effect of Liushen Pill （六神丸） on the Constitution and Diversity of Upper Respiratory Tract Flora in Healthy People

更新时间：2021-11-29

- Effect of Liushen Pill （六神丸） on the Constitution and Diversity of Upper Respiratory Tract Flora in Healthy People
- Journal of Traditional Chinese Medicine Vol. 62, Issue 23, Pages: 2063-2067(2021)
- 作者机构：
  
  首都医科大学附属北京中医医院，北京市东城区美术馆后街23号，100010
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2021.23.008
  CLC：
- Received：04 June 2021，
  
  Revised：2021-08-25，
  
  Published：02 December 2021
- 稿件说明：
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徐霄龙,陈奕杉,白云静等.六神丸对健康人上呼吸道菌群结构和多样性的影响[J].中医杂志,2021,62(23):2063-2067.

XU Xiaolong,CHEN Yishan,BAI Yunjing,et al.Effect of Liushen Pill （六神丸） on the Constitution and Diversity of Upper Respiratory Tract Flora in Healthy People[J].Journal of Traditional Chinese Medicine,2021,62(23):2063-2067.
徐霄龙,陈奕杉,白云静等.六神丸对健康人上呼吸道菌群结构和多样性的影响[J].中医杂志,2021,62(23):2063-2067. DOI： 10.13288/j.11-2166/r.2021.23.008.

XU Xiaolong,CHEN Yishan,BAI Yunjing,et al.Effect of Liushen Pill （六神丸） on the Constitution and Diversity of Upper Respiratory Tract Flora in Healthy People[J].Journal of Traditional Chinese Medicine,2021,62(23):2063-2067. DOI： 10.13288/j.11-2166/r.2021.23.008.

摘要

目的

基于16S rRNA高通量测序技术探讨六神丸对健康人上呼吸道菌群微生态的影响。

方法

选择2020年11月2日至11月20日在北京中医药大学及首都医科大学招募的60名健康受试者为研究对象，随机分为对照组和六神丸组各30例。对照组不给予药物干预；六神丸组给予六神丸含服3粒+口服7粒，每日3次，共7天。对照组于招募后第8天、六神丸组于服药后第8天采集受试者咽拭子样本进行16S rRNA高通量测序检测，分析Alpha多样性、Beta多样性和菌群结构差异。

结果

与对照组比较，六神丸组Chao1指数和Shannon指数增高（

＜0.05）；对照组和六神丸组非度量多维标定（NMDS）组间分布具有差异，NMDS1胁强系数=0.1590。厚壁菌门、拟杆菌门、变形菌门、梭杆菌门、放线菌门和髌骨细菌门为两组在门水平的优势菌。与对照组比较，六神丸组疣微菌门、硝化螺旋菌门、芽单胞菌门、蓝藻细菌门、绿弯菌门和酸杆菌门相对丰度增高（

＜0.05）；变形菌门、埃普西隆杆菌门和拟杆菌门相对丰度降低（

＜0.05）。

结论

六神丸对上呼吸道菌群的多样性、丰度和结构均有调节作用，提示六神丸可能通过改善菌群失调对某些呼吸系统疾病起到预防或治疗作用。

Abstract

Objective

To investigate the effect of

Liushen Pill

（六神丸） on the microecology of upper respiratory tract flora in healthy people based on 16S rRNA high-throughput sequencing technology.

Methods

A total of 60 healthy subjects were recruited in Beijing University of Chinese medicine and Capital Medical University from November 2nd to 20th， 2020， and were randomly divided into control group and

Liushen Pill

group with 30 in each group. No medication was given to the control group and the

Liushen Pill

group was treated with 3 pills （sucked） + 7 pills （swallowed） of

Liushen Pill

for 7 days， three times a day.On the 8th day of inclusion or after administration， throat swab samples were collected from the control group or

Liushen Pill

group through 16S rRNA high-throughput sequencing， to analyze alpha diversity， beta diversity and flora constitution.

Results

Compared to those in the control group， the Chao1 index and Shannon index in the

Liushen Pill

group were higher （

＜0.05）. The distribution of the non-metric multidimensional scaling （NMDS） was significantly different between the control group and the

Liushen Pill

group， with NMDS1 stress coefficient=0.1590.

Firmicutes

，

Bacteroidetes

，

Proteobacteria

，

Fusobacteria

，

Actinobacteria

and

Patescibacteria

were the dominant phyla in both groups. Compared to the control group， the relative abundance of

Verrucomicrobia

，

Nitrospirae， Gemmatimonadetes

，

Cyanobacteria

，

Chloroflexi

and

Acidobacteria

in the

Liushen Pill

group significantly increased （

＜0.05）， and the relative abundance of

Proteobacteria

，

Epsilonbacteraeota

and

Bacteroidetes

significantly decreased （

＜0.05）.

Conclusion

Liushen Pill

has effects on the diversity， abundance and constituion of the upper respiratory tract flora in healthy people. It is suggested that

Liushen Pill

may have potential effect on preventing and treating certain respiratory diseases.

关键词

Keywords

references

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Effect of Liushen Pill （六神丸） on the Constitution and Diversity of Upper Respiratory Tract Flora in Healthy People

DOI：10.13288/j.11-2166/r.2021.23.008

摘要

Abstract

关键词

Keywords

references