Effect of Different Stimulation Strength of Acupuncture on Pain and the Content of Prostaglandin and Oxytocin in Dysmenorrhea-like Model Rats

Haimei XIE; Yijiang LIU; Pei WANG; Hongwen YUAN; Yue SONG; Yang LIU; Jinfeng LI; Min LI

doi:10.13288/j.11-2166/r.2022.05.014

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Effect of Different Stimulation Strength of Acupuncture on Pain and the Content of Prostaglandin and Oxytocin in Dysmenorrhea-like Model Rats

更新时间：2022-02-28

- Effect of Different Stimulation Strength of Acupuncture on Pain and the Content of Prostaglandin and Oxytocin in Dysmenorrhea-like Model Rats
- Journal of Traditional Chinese Medicine Vol. 63, Issue 5, Pages: 475-480(2022)
- 作者机构：
  
  1.首都医科大学附属北京潞河医院，北京市通州区新华南路82号，101149
  2.首都医科大学中医药学院
  3.北京中医药大学针灸推拿学院
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2022.05.014
  CLC：
- Received：23 May 2021，
  
  Revised：2021-12-27，
  
  Published：02 March 2022
- 稿件说明：
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谢海梅,柳依江,王培等.针刺强度对类痛经模型大鼠镇痛效应、前列腺素及缩宫素含量的影响[J].中医杂志,2022,63(05):475-480.

XIE Haimei,LIU Yijiang,WANG Pei,et al.Effect of Different Stimulation Strength of Acupuncture on Pain and the Content of Prostaglandin and Oxytocin in Dysmenorrhea-like Model Rats[J].Journal of Traditional Chinese Medicine,2022,63(05):475-480.
谢海梅,柳依江,王培等.针刺强度对类痛经模型大鼠镇痛效应、前列腺素及缩宫素含量的影响[J].中医杂志,2022,63(05):475-480. DOI： 10.13288/j.11-2166/r.2022.05.014.

XIE Haimei,LIU Yijiang,WANG Pei,et al.Effect of Different Stimulation Strength of Acupuncture on Pain and the Content of Prostaglandin and Oxytocin in Dysmenorrhea-like Model Rats[J].Journal of Traditional Chinese Medicine,2022,63(05):475-480. DOI： 10.13288/j.11-2166/r.2022.05.014.

摘要

目的

观察不同针刺强度对类痛经模型大鼠的镇痛效应，探讨其可能的作用机制。

方法

40只SD雌性大鼠随机分为空白组、模型组、弱刺激组及强刺激组，每组10只。除空白组外，其余各组均采用苯甲酸雌二醇和缩宫素注射法建立类痛经模型。空白组与模型组仅抓取固定，强刺激组采用粗针、深刺、施手法，弱刺激组采用细针、浅刺、不施手法，两组均刺激双侧“三阴交”穴20 min，起针后即刻观察大鼠扭体反应。采用ELISA法检测血清及子宫组织前列腺素F

2α

（PGF

2α

）、前列腺素E

（PGE

）及缩宫素（OT）含量，采用化学法检测血清钙离子（Ca

）含量。

结果

与空白组比较，模型组大鼠扭体潜伏期明显缩短、扭体评分升高，血清及子宫组织PGF

、PGF

/PGE

、OT及血清Ca

含量均显著升高，而血清及子宫组织PGE

含量显著降低（

＜0.01）。与模型组比较，强刺激组与弱刺激组大鼠扭体潜伏期均明显延长、扭体评分均显著降低，子宫组织PGF

含量、血清及子宫组织中PGF

/PGE

及OT含量均显著降低，血清PGE

含量显著升高（

＜0.05或

＜0.01），强刺激组血清PGF

、Ca

含量显著降低（

＜0.05）。与弱刺激组比较，强刺激组扭体潜伏期显著延长、扭体评分显著降低（

＜0.05或

＜0.01），且强刺激组血清PGF

显著低于弱刺激组（

＜0.05）。

结论

针刺能缓解类痛经模型大鼠的疼痛程度，且强刺激优于弱刺激。其作用机制可能是通过调节血清和子宫组织中PGF

2α

、PGE

及OT含量，降低血清Ca

含量，从而改善平滑肌异常收缩而实现的。

Abstract

Objective

To observe the analgesic effect of acupuncture with different stimulation strength on dysmenorrhea-like rats and to explore the possible mechanism.

Methods

Forty SD female rats were randomly divided into blank group， model group， weak-stimulation acupuncture （WA） group and strong-stimulation acupuncture （SA） group， with 10 rats in each group. Dysmenorrhea-like model was established by injection of estradiol benzoate and oxytoxin in all groups except for the blank group. Rats in the blank group and the model group were only grasped and fixed without interventions； a 20-minute deep needling at bilateral sanyinjiao （SP6） acupoints with thick needles added by manipulation techniques was given to the SA group， while a 20-minute superficial needling at the same acupoints with a thin needles without manipulation techniques was given to the WA group. Writhing response of the rats was observed after withdrawal of needles. The content of prostaglandin F

2α

（PGF

2α

）， prostaglandin E

（PGE

）

and oxytocin （OT） in serum and uterine tissue were detected by ELISA method， while the content of Ca

in serum was detected by chemical methods.

Results

Compared to those in the blank group， the latent period of writhing in the model group was significantly shortened， and the writhing score was increased； the content of PGF

， PGF

/PGE

， and OT in serum and uterine tissue， and the content of Ca

in serum were significantly increased； the PGE

content in serum and uterine tissue were reduced in the model group （

＜0.01）. Compared to the model group， the SA and WA groups had a significantly prolonged latent period of writhing， decreased writhing scores， PGF

content in uterine tissue， PGF

/PGE

and OT content in serum and uterine tissue， as well as increased PGE

content in serum （

＜0.05 or

＜0.01）. The SA group had significantly decreased PGF

and Ca

content in serum （

＜0.05）. The latent period of writhing in the SA group was significantly longer than that in the WA group， while the writhing score was lower （

＜0.05 or

＜0.01）， and the PGF

content in serum was obviously lower in the SA group than that in the WA group （

＜0.05）.

Conclusion

Acupuncture could relieve the pain in dysmenorrhea-like rats， and strong-stimulation has better effects than weak-stimulation. The possible mechanism may be explained by that acupuncture can regulate the content of PGF

， PGE

and OT in serum and uterine tissue， and reduce the content of Ca

in serum， thereby improving the contraction of smooth muscle.

关键词

Keywords

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