Effects of Kushen （<italic style="font-style: italic">Radix Sophorae Flavescentis</italic>） on the Expression of PAR-2 and TrK-A Protein in Skin Lesions of Atopic Dermatitis Model Mice

Xue SONG; Chengxiang WANG; Jiaojiao YUAN; Xinrong ZHANG; Guomi WANG; Shanshan GAO; Zhanxue SUN

doi:10.13288/j.11-2166/r.2022.09.014

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Effects of Kushen （Radix Sophorae Flavescentis） on the Expression of PAR-2 and TrK-A Protein in Skin Lesions of Atopic Dermatitis Model Mice

更新时间：2022-04-25

- Effects of Kushen （Radix Sophorae Flavescentis） on the Expression of PAR-2 and TrK-A Protein in Skin Lesions of Atopic Dermatitis Model Mice
- Journal of Traditional Chinese Medicine Vol. 63, Issue 9, Pages: 869-874(2022)
- 作者机构：
  
  1.北京中医药大学第三附属医学院，北京市朝阳区安外小关街51号，100029
  2.北京中医药大学东直门医院
  3.北京市昌平区东小口社区卫生服务中心
  4.北京中医药大学
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2022.09.014
  CLC：
- Received：28 September 2021，
  
  Revised：2022-01-15，
  
  Published：02 May 2022
- 稿件说明：
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宋雪,王成祥,袁姣姣等.苦参对特应性皮炎模型小鼠皮损组织PAR-2、TrK-A蛋白表达的影响[J].中医杂志,2022,63(09):869-874.

SONG Xue,WANG Chengxiang,YUAN Jiaojiao,et al.Effects of Kushen （Radix Sophorae Flavescentis） on the Expression of PAR-2 and TrK-A Protein in Skin Lesions of Atopic Dermatitis Model Mice[J].Journal of Traditional Chinese Medicine,2022,63(09):869-874.
宋雪,王成祥,袁姣姣等.苦参对特应性皮炎模型小鼠皮损组织PAR-2、TrK-A蛋白表达的影响[J].中医杂志,2022,63(09):869-874. DOI： 10.13288/j.11-2166/r.2022.09.014.

SONG Xue,WANG Chengxiang,YUAN Jiaojiao,et al.Effects of Kushen （Radix Sophorae Flavescentis） on the Expression of PAR-2 and TrK-A Protein in Skin Lesions of Atopic Dermatitis Model Mice[J].Journal of Traditional Chinese Medicine,2022,63(09):869-874. DOI： 10.13288/j.11-2166/r.2022.09.014.

摘要

目的

探讨苦参治疗特应性皮炎的可能作用机制。

方法

60只BALB/c雄性小鼠随机分为正常组、模型组、西替利嗪组和苦参高、中、低剂量组，每组10只。除正常组外，其余各组小鼠用卵清蛋白（OVA）诱导特应性皮炎模型，造模成功后，苦参高、中、低剂量组小鼠分别给予苦参3.75、2.50、1.25 g/（kg·d）灌胃，西替利嗪组给予西替利嗪片10 mg/（kg·d）灌胃，正常组和模型组给予10 ml/（kg·d）生理盐水灌胃。各组均每天灌胃2次，连续3周。分别于干预1、2、3周时观察小鼠每30min内搔抓次数；干预3周后采用HE染色观察皮损组织病理形态学变化，测定血清中P物质（SP）、降钙素基因相关肽（CGRP）、前列腺素E2（PGE2）、白三烯B4（LTB4）浓度，分别采用免疫印记和免疫组化法检测皮损组织中蛋白酶激活受体2（PAR-2）和酪氨酸激酶A（TrK-A）蛋白表达。

结果

与正常组同时间相比，模型组小鼠干预1、2、3周时搔抓次数显著增加（

＜0.01）；HE染色显示小鼠的表皮过度角化、真皮层增厚、炎细胞浸润；血清中PGE2、CGRP、LTB4和SP浓度明显升高；皮损组织中PAR-2和TrK-A蛋白表达增多（

＜0.05或

＜0.01）。与模型组同时间比较，除苦参低剂量组干预1周时外，西替利嗪组和苦参各剂量组各时间点小鼠搔抓次数显著降低（

＜0.01）；西替利嗪组和苦参各剂量组小鼠的表皮和真皮比模型组明显变薄，炎症细胞浸润明显减轻，且以西替利嗪组和苦参高剂量组改善明显；西替利嗪组和苦参各剂量组小鼠血清中PGE2、CGRP、LTB4和SP浓度均降低，皮损组织中PAR-2和TrK-A蛋白表达降低（

＜0.05或

＜0.01）。与苦参高剂量组同时间比较，苦参低剂量组小鼠搔抓次数明显升高（

＜0.05）。西替利嗪组及苦参各剂量组组间两两比较，小鼠血清中PGE2、CGRP、LTB4和SP浓度，皮损组织中PAR-2和TrK-A蛋白表达差异均无统计学意义（

P＞

0.05）。

结论

苦参可以改善特应性皮炎瘙痒程度，其机制可能与抑制皮损组织中PAR-2和TRK-A蛋白表达有关。

Abstract

Objective

To explore the possible mechanism of Kushen （

Radix Sophorae Flavescentis

） in the treatment of atopic dermatitis （AD）.

Methods

Sixty BALB/c male mice were randomly divided into normal group， model group， cetirizine group and Kushen high-， medium- and low-dose groups， with 10 mice in each group. Except for the normal group， mice were induced with ovalbumin （OVA） to develop the atopic dermatitis model. After successful modeling， the mice in the Kushen high-， medium- and low- dose groups were given 3.75， 2.50， and 1.25 g/（kg·d） of Kushen by gavage respectively； mice in the cetirizine group were given 10 mg/（kg·d） of cetirizine tablets， while those in the normal group and model group received 10 ml/（kg·d） of normal saline by gavage. All the interventions were administered twice a day for three consecutive weeks. The number of scratches over 30 minutes was observed after 1-， 2- and 3-week treatment. After 3-week treatment， HE staining was used to assess the pathological changes of skin lesions， and the concentrations of substance P （SP）， calcitonin gene-related peptide （CGRP）， prostaglandin E2 （PGE2） and leukotriene B4 （LTB4） in serum were determined； immunoblotting and immunohistochemistry were used to detect the protein expressions of protease-activated receptor 2 （PAR-2） and tyrosine kinase A （TrK-A） in skin lesions， respectively.

Results

Compared to the results measured after 1-， 2- and 3-week treatment in the normal group， the number of scratches significantly increased at the corresponding time in the model group （

＜0.01）. Moreover， HE staining results showed that the epidermis in the model group was hyperkeratinized； the dermis was thickened， and inflammatory cells were infiltrated in the mice； the concentrations of PGE2， CGRP， LTB4 and SP significantly increased， and PAR2 and TrK-A protein expressions in skin lesions significantly increased （

＜0.05 or

＜0.01）. Compared to the results measured after 1-， 2， and 3-week treatment in the model group， the number of scratches significantly decreased at the corresponding time in the cetirizine group and three Kushen groups， except for the result of low-dose Kushen group after 1-week treatment （

＜0.01）. Moreover， the cetirizine group and three Kushen groups had thinner epidermis and dermis， and reduced inflammatory cell infiltration than the model group， and more improvement was seen in the cetirizine group and high-dose Kushen group； the concentrations of PGE2， CGRP， LTB4 and SP in the serum， as well as the protein expressions of PAR2 and TrK-A in the skin lesions in the cetirizine group and three Kushen groups significantly decreased （

＜0.05 or

＜0.01）. Compared to the high-dose group， the low-dose Kushen group had significantly increased number of scratches （

＜0.05）. There were no significant differences in the concentrations of PGE2， CGRP， LTB4 and SP in the serum， and the protein expressions of PAR2 and TrK-A in the skin lesions for the pairwise comparisons between the cetirizine group and three Kushen groups （

＞0.05）.

Conclusion

Kushen can improve the itching degree of AD， and the mechanism may be related to the inhibition of PAR-2 and TRK-A protein expression in skin lesions.

关键词

Keywords

references

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Effects of Kushen （Radix Sophorae Flavescentis） on the Expression of PAR-2 and TrK-A Protein in Skin Lesions of Atopic Dermatitis Model Mice

DOI：10.13288/j.11-2166/r.2022.09.014

摘要

Abstract

关键词

Keywords

references