Effect of <italic style="font-style: italic">Jiaotai Pills </italic>（交泰丸） on Sleep Stage and Sleep Quality in Insomnia Rats with Syndrome of Non-interaction between the Heart and the Kidney

Xueai ZENG; Chunquan ZHOU; Xinzi GUO; Min ZHANG; Yifan ZHANG; Junshan HUANG

doi:10.13288/j.11-2166/r.2022.11.015

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Effect of Jiaotai Pills （交泰丸） on Sleep Stage and Sleep Quality in Insomnia Rats with Syndrome of Non-interaction between the Heart and the Kidney

更新时间：2022-05-28

- Effect of Jiaotai Pills （交泰丸） on Sleep Stage and Sleep Quality in Insomnia Rats with Syndrome of Non-interaction between the Heart and the Kidney
- Journal of Traditional Chinese Medicine Vol. 63, Issue 11, Pages: 1080-1087(2022)
- 作者机构：
  
  1.福建省中医药科学院，福建省福州市五四路282号，350003
  2.福建中医药大学
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2022.11.015
  CLC：
- Received：19 October 2021，
  
  Revised：2022-03-22，
  
  Published：02 June 2022
- 稿件说明：
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曾雪爱,周春权,郭昕子等.交泰丸对心肾不交失眠模型大鼠睡眠时相和睡眠质量的影响[J].中医杂志,2022,63(11):1080-1087.

ZENG Xueai,ZHOU Chunquan,GUO Xinzi,et al.Effect of Jiaotai Pills （交泰丸） on Sleep Stage and Sleep Quality in Insomnia Rats with Syndrome of Non-interaction between the Heart and the Kidney[J].Journal of Traditional Chinese Medicine,2022,63(11):1080-1087.
曾雪爱,周春权,郭昕子等.交泰丸对心肾不交失眠模型大鼠睡眠时相和睡眠质量的影响[J].中医杂志,2022,63(11):1080-1087. DOI： 10.13288/j.11-2166/r.2022.11.015.

ZENG Xueai,ZHOU Chunquan,GUO Xinzi,et al.Effect of Jiaotai Pills （交泰丸） on Sleep Stage and Sleep Quality in Insomnia Rats with Syndrome of Non-interaction between the Heart and the Kidney[J].Journal of Traditional Chinese Medicine,2022,63(11):1080-1087. DOI： 10.13288/j.11-2166/r.2022.11.015.

摘要

目的

观察交泰丸对心肾不交失眠模型大鼠睡眠质量的影响。

方法

21只SD大鼠随机分为空白组、模型组、交泰丸组，每组7只。除空白组外，其余各组大鼠采用复合刺激+腹腔注射对氯苯丙氨酸（PCPA）建立心肾不交失眠大鼠模型，造模后交泰丸组给予交泰丸1.1 g/（kg·d）灌胃，空白组和模型组给予蒸馏水10 ml/（kg·d）灌胃，各组均灌胃7天。通过动物睡眠生物解析系统记录脑电（EEG）和肌电（EMG）信号24 h，分析大鼠夜晚（19：00～次日7：00）和白天（次日7：00～19：00）觉醒（Wake）量、非快速眼动（NREM）睡眠总量和快速眼动（REM）睡眠总量，各睡眠时相的转换次数，不同时程NREM睡眠、REM睡眠和Wake出现次数，脑电频谱密度；记录结束后测定大鼠肾上腺指数，ELISA法检测大鼠血清促肾上腺皮质激素（ACTH）、促肾上腺皮质激素释放激素（CRH）含量。

结果

与空白组比较，模型组大鼠肾上腺指数升高、血清ACTH含量升高（

＜0.05或

＜0.01），但CRH含量差异无统计学意义（

＞0.05）；夜晚和白天NREM睡眠总量均减少，REM睡眠总量减少，Wake总量增多（

＜0.05或

＜0.01），主要表现为10～20 s和30～50 s NREM睡眠次数显著增多，120～230 s和240～470 s NREM睡眠次数显著减少（

＜0.05或

＜0.01），NREM睡眠转换为Wake的次数增加，NREM睡眠转换为REM睡眠的次数减少，REM睡眠转换为Wake的次数减少（

＜0.05或

＜0.01）。与模型组比较，交泰丸组肾上腺指数下降、血清ACTH含量下降（

＜0.05），但CRH差异无统计学意义（

＞0.05）；夜晚和白天NREM睡眠总量均显著增加，Wake总量均显著减少（

＜0.01），主要表现为10～20 s和30～50 s NREM睡眠次数显著减少，120～230 s和240～470 s NREM睡眠次数显著增加（

＜0.01），NREM睡眠与Wake之间的转换次数减少（

＜0.01）。与空白组比较，交泰丸组大鼠NREM睡眠的Delta波密度和REM睡眠的Theta波密度差异无统计学意义（

＞0.05）。

结论

交泰丸主要通过增加较长时程（120～230 s、240～470 s）NREM睡眠，减少NREM睡眠与Wake之间的转换次数，提高睡眠时间和睡眠质量，且诱导的睡眠类似于生理性睡眠。

Abstract

Objective

To evaluate the effect of

Jiaotai Pills

（交泰丸） on the sleep stage and sleep quality in insomnia model rats with syndrome of non-interaction between the heart and the kidney.

Methods

Twenty-one SD rats were randomly divided into control group， model group and

Jiaotai Pills

group， with 7 rats in each group. Except for the control group， the rat model of insomnia with non-interaction between the heart and the kidney was established through compound stimulation and intraperitoneal injection of para-chlorophenylalanine （PCPA）. After modeling， the

Jiaotai Pills

group was administrated with 1.1 g/kg of

Jiaotai Pills

by gavage， while the control group and model group were given 10 mL/kg of distilled water by gavage， once a day for 7 consecutive days. Electroencephalogram（EEG） and electromyogram （EMG） were recorded through animal sleep bioanalytical system. The total amount of night ［（19：00-07：00 （+1）］ and day ［（07：00（+1）-19：00（+1）］ wake， non-rapid eye movement （NREM） sleep and rapid eye movement （REM） sleep， the number of sleep stage transitions， the episode number of NREM sleep， REM sleep and wake， and EEG power density were analyzed. After recording， the adrenal index was determined， and the adrenocorticotropic hormone （ACTH） and corticotropin-releasing hormone （CRH） levels in serum were detected through ELSA.

Results

Compared to those in the control group， the adrenal index and serum ACTH level in the model group significantly increased （

＜0.05 or

＜0.01）， while there was no difference between groups in CRH level （

＞0.05）； the total amount of night and day NREM sleep and REM sleep were reduced， while the wake amount increased （

＜0.05 or

＜0.01）， with increased episode number of 10-20 s and 30-50 s NREM sleep and decreased 120-230 s and 240-470 s NREM sleep （

＜0.05 or

＜0.01）， as well as increased number of NREM sleep transiting into wake and decreased number of NREM sleep transiting into REM sleep and that of REM sleep transiting into wake （

＜0.05 or

＜0.01）. Compared to those in the model group， the adrenal index and ACTH level in serum in the

Jiaotai Pills

group were reduced （

＜0.05）， but there was no difference in CRH （

＞0.05）； the total amount of night and day NREM sleep increased while the wake amount was reduced significantly （

＜0.01）， with decreased episode number of 10-20 s and 30-50 s NREM sleep and increased episode number of 120-230 s and 240-470 s NREM sleep （

＜0.01）， as well as reduced number of NREM sleep transiting into wake （

＜0.01）. There were no significant differences in the Delta wave density of NREM sleep and the Theta wave density of REM sleep between the

Jiaotai Pills

group and control group （

＞0.05）.

Conclusion

Jiaotai Pills

can improve sleep stage and sleep quality mainly by increasing long duration NREM sleep （120-230 s and 240-470 s） and reducing the transition number between NREM sleep and wake， and the induced sleep is similar to physiological sleep.

关键词

Keywords

references

张斌．中国失眠障碍诊断和治疗指南［M］．北京：人民卫生出版社， 2016 ： 8 ．

黄运芳，毕欣宁，郑伟，等 . 交泰丸对心肾不交型失眠大鼠中枢及外周神经递质的调控作用［J］. 中国中药杂志， 2020 ， 45 （ 9 ）： 2172 - 2179 .

LI ZH ， MA PK ， HUANG YF ， et al . Jiaotai pill（交泰丸）alleviates insomnia through regulating monoamine and organic cation transporters in rats ［J］. Chin J Integr Med ， 2021 ， 27 （ 3 ）： 183 - 191 .

龚梦鹃，岳贺，周祥羽，等 . 交泰丸干预对氯苯丙氨酸致大鼠失眠的作用机制研究［J］. 世界中西医结合杂志， 2017 ， 12 （ 9 ）： 1230 - 1234 .

洪曹栋，尹嘉，张萌，等 . 基于网络药理学探讨交泰丸治疗失眠的作用机制［J］. 中西医结合心脑血管病杂志， 2020 ， 18 （ 10 ）： 1522 - 1530 .

邱梅红，岳小芳，徐昕红，等 . 小鼠睡眠生物解析系统与应用［J］. 中国药理学通报， 2008 ， 24 （ 1 ）： 20 - 23 .

郜红利，涂星，卢映，等 . 心肾不交所致失眠大鼠模型［J］. 中成药， 2014 ， 36 （ 6 ）： 1138 - 1141 .

杨钰涵，孙雨，王珺，等 . 中医病证相符的大鼠心肾不交失眠模型的建立及其血清代谢组学研究［J］. 中国中药杂志， 2020 ， 45 （ 2 ）： 383 - 390 .

陈奇 . 中药药理研究方法学［M］. 3版 . 北京：人民卫生出版社， 2011 ： 1261 - 1263 .

王硕，钱晶，齐文升 . 心肾不交之不寐证辨证治疗体会［J］. 北京中医药， 2013 ， 32 （ 2 ）： 111 - 112 .

陆伟珍，张雯静，王余民，等 . 交泰丸治疗失眠症的疗效研究［J］. 世界睡眠医学杂志， 2018 ， 5 （ 9 ）： 1018 - 1021 .

肖成荣，马增春，李海静，等 . PCPA失眠大鼠模型的制作及其机制［J］. 毒理学杂志， 2007 ， 21 （ 4 ）： 326 .

赵俊云，杨晓敏，胡秀华，等 . 失眠动物模型HPA轴和表观遗传修饰的变化及交泰丸的干预作用［J］. 中医药学报， 2018 ， 46 （ 4 ）： 19 - 21 .

MEERLO P ， DE BRUIN EA ， STRIJKSTRA AM ， et al . A social conflict increases EEG slow-wave activity during subsequent sleep ［J］. Physiol Behav ， 2001 ， 73 （ 3 ）： 331 - 335 .

LIU Z ， XU XH ， LIU TY ， et al . Safranal enhances non-rapid eye movement sleep in pentobarbital-treated mice ［J］. CNS Neurosci Ther ， 2012 ， 18 （ 8 ）： 623 - 630 .

YOON M ， CHO S . Triphlorethol A， a dietary polyphenol from seaweed， decreases sleep latency and increases non-rapid eye movement sleep in mice ［J］. Mar Drugs ， 2018 ， 16 （ 5 ）： 139 .

QU WM ， YUE XF ， SUN Y ， et al . Honokiol promotes non-rapid eye movement sleep via the benzodiazepine site of the GABA（A） receptor in mice ［J］. Br J Pharmacol ， 2012 ， 167 （ 3 ）： 587 - 598 .

TOBLER I ， KOPP C ， DEBOER T ， et al . Diazepam-induced changes in sleep： role of the alpha 1 GABA（A） receptor subtype ［J］. PNAS ， 2001 ， 98 （ 11 ）： 6464 - 6469 .

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Effect of Jiaotai Pills （交泰丸） on Sleep Stage and Sleep Quality in Insomnia Rats with Syndrome of Non-interaction between the Heart and the Kidney

DOI：10.13288/j.11-2166/r.2022.11.015

摘要

Abstract

关键词

Keywords

references