Effects of Bloodletting at “<italic style="font-style: italic">Baihui </italic>（DU 20）” and “<italic style="font-style: italic">Sishencong</italic> （EX-HN1）” on Cerebral Microinfarcts and Neurovascular Coupling in Intracranial Hypoperfusion Model Rats

Qingyin FENG; Shaosong WANG; Xin WANG; Qianqian LI; Yingxue CUI; Wenxu ZHANG; Ziyi WANG; Panlin CEN

doi:10.13288/j.11-2166/r.2022.19.014

您当前的位置：

首页 >

文章列表页 >

Effects of Bloodletting at “Baihui （DU 20）” and “Sishencong （EX-HN1）” on Cerebral Microinfarcts and Neurovascular Coupling in Intracranial Hypoperfusion Model Rats

更新时间：2022-09-26

- Effects of Bloodletting at “Baihui （DU 20）” and “Sishencong （EX-HN1）” on Cerebral Microinfarcts and Neurovascular Coupling in Intracranial Hypoperfusion Model Rats
- Journal of Traditional Chinese Medicine Vol. 63, Issue 19, Pages: 1872-1879(2022)
- 作者机构：
  
  首都医科大学附属北京中医医院，北京市东城区美术馆后街23号，100010
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2022.19.014
  CLC：
- Received：22 February 2022，
  
  Revised：2022-03-31，
  
  Published：02 October 2022
- 稿件说明：
移动端阅览
冯晴殷,王少松,王鑫等.“百会”“四神聪”放血对颅内低灌注模型大鼠脑微梗死及神经血管耦合功能的影响[J].中医杂志,2022,63(19):1872-1879.

FENG Qingyin,WANG Shaosong,WANG Xin,et al.Effects of Bloodletting at “Baihui （DU 20）” and “Sishencong （EX-HN1）” on Cerebral Microinfarcts and Neurovascular Coupling in Intracranial Hypoperfusion Model Rats[J].Journal of Traditional Chinese Medicine,2022,63(19):1872-1879.
冯晴殷,王少松,王鑫等.“百会”“四神聪”放血对颅内低灌注模型大鼠脑微梗死及神经血管耦合功能的影响[J].中医杂志,2022,63(19):1872-1879. DOI： 10.13288/j.11-2166/r.2022.19.014.

FENG Qingyin,WANG Shaosong,WANG Xin,et al.Effects of Bloodletting at “Baihui （DU 20）” and “Sishencong （EX-HN1）” on Cerebral Microinfarcts and Neurovascular Coupling in Intracranial Hypoperfusion Model Rats[J].Journal of Traditional Chinese Medicine,2022,63(19):1872-1879. DOI： 10.13288/j.11-2166/r.2022.19.014.

摘要

目的

探讨“百会”“四神聪”放血对颅内低灌注模型大鼠认知障碍的影响及可能作用机制。

方法

30只SD 大鼠随机分为实验组、模型组、空白组各10只。实验组、模型组采用双侧颈总动脉结扎法建立颅内低灌注大鼠模型。实验组造模1周后开始给予“百会”“四神聪”放血，每周一、三、五放血，干预4周，共12次；模型组及空白组大鼠不做干预。各组大鼠干预4周后采用Morris水迷宫评价大鼠认知行为学改变，包括隐蔽平台实验（持续5天，记录每天逃避潜伏期时间）和空间探索实验（隐蔽平台实验结束24 h后开始，记录大鼠平台象限停留时间、平台位置穿越次数、平台象限游泳距离）。水迷宫实验完成后取大鼠脑组织进行HE染色，根据染色结果统计脑微梗死灶数量，观察脑白质损伤情况并分为0度、Ⅰ度、Ⅱ度；免疫组织化学法测定大鼠脑组织髓鞘碱性蛋白（MBP）、神经元型一氧化氮合酶（nNOS）、花生二烯酸（AA）、神经胶质抗原2（NG2）蛋白表达；ELISA法检测大鼠外周血清一氧化氮（NO）含量。

结果

与空白组同时间比较，模型组和实验组大鼠Morris水迷宫隐蔽平台实验第5天逃避潜伏期延长（

＜0.05）；与模型组同时间比较，实验组大鼠第5天逃避潜伏期缩短（

＜0.05）。各组大鼠Morris水迷宫空间探索实验平台位置穿越次数、平台象限游泳距离比较差异无统计学意义（

＞0.05）；与空白组比较，模型组大鼠平台象限停留时间缩短（

＜0.05）；与模型组比较，实验组大鼠平台象限停留时间延长（

＜0.05）。与空白组比较，模型组大鼠脑组织中脑微梗死灶数量增多，脑白质损伤程度分级以Ⅱ度为多，脑组织MBP、NG2、AA蛋白表达和外周血清NO含量升高（

＜0.05）；与模型组比较，实验组大鼠脑白质损伤程度分级以0度和Ⅰ度为主，脑组织MBP、NG2蛋白表达和外周血清NO含量降低，AA蛋白表达升高（

＜0.05）；与模型组比较，实验组大鼠脑微梗死灶数量有减少趋势但两组比较差异无统计学意义（

＞0.05）。

结论

“百会”“四神聪”放血可以改善颅内低灌注模型大鼠的认知功能，减轻脑白质损伤程度，其机制可能与调节皮质神经元介导的神经血管耦合通路相关。

Abstract

Objective

To investigate the effect and possible mechanism of bloodletting at

Baihui

（DU 20） and

Sishencong

（EX-HN1） on cognitive impairment caused by hypoperfusion.

Methods

Thirty SD rats were randomly divided into treatment group， model group and blank group with 10 rats in each group. Intracranial hypoperfusion rat model was established in the treatment group and the model group by bilateral common carotid artery ligation. One week after successful modeling， bloodletting treatment at

Baihui

（DU 20） and

Sishencong

（EX-HN1） was given to the rats in the treatment group， administered on every Monday， Wednesday and Friday for 4 weeks， 12 times in total. No intervention was performed in the model group and blank group. Morris water maze was used to evaluate the cognitive behavior changes of rats after 4-week intervention， including hidden platform experiment continuously for five days to record daily escape latency time， and space exploration experiment 24 h later to record the time in platform quadrant， number of platform crossings，and swimming distance in the platform quadrant. After water maze experiment， the brain tissues of the selected rats were sacrificed for HE staining to calculate the number of cerebral microinfarcts and observe the white matter injury （grading as 0， Ⅰ and Ⅱ）. The protein expression of myelin basic protein （MBP）， neuronal nitric oxide synthase （nNOS）， arachidonic acid （AA） and glial antigen 2 （NG2） were determined by immunohistochemistry. The serum NO content was detected by ELISA.

Results

Compared to that of the blank group， the latency time of escape in Morris water maze test was significantly prolonged in model group and treatment group （

＜0.05）， and prolonged latency time was also found in the model group compared to treatment group （

＜0.05）. There was no significant difference in the number of platform crossings and the swimming distance in the platform quadrant between groups （

＞0.05）. The time in platform quadrant in the model group was shorter than that in the blank group （

＜0.05）， and was also shortened compared to that of treatment group （

＜0.05）. The number of cerebral microinfarcts in brain tissue increased， with mostly grade Ⅱ damage of cerebral white matter， and the protein expression of MBP， NG2 and AA in the brain tissue and peripheral serum NO content increased in the model group than the blank group （

＜0.05）. Compared to those in the model group， the damage degree of cerebral white matter was mainly at grade 0 and I， and the protein expression of MBP and NG2 in brain tissue and peripheral serum NO content decreased， while the expression of AA protein increased in the treatment group （

＜0.05）. The number of cerebral microinfarcts showed a decreasing trend in the treatment group compared to the model group， but there was no statistical difference （

＞0.05）.

Conclusion

Bloodletting at

Baihui

（DU 20） and

Sishencong

（EX-HN1） can improve the cognitive function of intracranial hypoperfusion model rats and alleviate the damage degree of cerebral white matter， and its mechanism may be related to the regulation of neurovascular coupling pathway mediated by cortical neurons.

关键词

Keywords

references

ZHANG L ， BIESSELS GJ ， HILAL S ， et al . Cerebral microinfarcts affect brain structural network topology in cognitively impaired patients ［J］. J Cereb Blood Flow Metab ， 2021 ， 41 （ 1 ）： 105 - 115 .

VAN VELUW SJ ， SHIH AY ， SMITH EE ， et al . Detection， risk factors， and functional consequences of cerebral microinfarcts ［J］. Lancet Neurol ， 2017 ， 16 （ 9 ）： 730 - 740 .

FERRO DA ， MUTSAERTS HJ ， HILAL S ， et al . Cortical microinfarcts in memory clinic patients are associated with reduced cerebral perfusion ［J］. J Cereb Blood Flow Metab ， 2020 ， 40 （ 9 ）： 1869 - 1878 .

FU R ， WANG Y ， WANG Y ， et al . The development of cortical microinfarcts is associated with intracranial atherosclerosis：data from the Chinese intracranial atherosclerosis study ［J］. J Stroke Cerebrovasc Dis ， 2015 ， 24 （ 11 ）： 2447 - 2454 .

王桂玲，程金莲，孙敬青 . 金针王乐亭治疗中风病经验［J］. 北京中医药， 2012 ， 31 （ 1 ）： 23 - 25 .

王少松，王麟鹏，马婷婷，等 . 醒神开窍针刺法对脑梗死急性期患者脑血管储备功能的影响［J］. 中医杂志， 2018 ， 59 （ 15 ）： 1314 - 1317 .

SHIH AY ， HYACINTH HI ， HARTMANN DA . Rodent models of cerebral microinfarct and microhemorrhage ［J］. Stroke ， 2018 ， 49 （ 3 ）： 803 - 810 .

WANG M ， ILIFF JJ ， LIAO Y ， et al . Cognitive deficits and delayed neuronal loss in a mouse model of multiple microinfarcts ［J］. J Neurosci ， 2012 ， 32 （ 50 ）： 17948 - 17960 .

中国针灸学会 . 实验动物常用穴位名称与定位第2部分：大鼠［J］. 针刺研究， 2021 ， 46 （ 4 ）： 351 - 352 .

UEHARA H ， YOSHIOKA H ， KAWASE S ， et al . A new model of white matter injury in neonatal rats with bilateral carotid artery occlusion ［J］. Brain Res ， 1999 ， 837 （ 1/2 ）： 213 - 220 .

WANG S ， YANG H ， ZHANG J ， et al . Efficacy and safety assessment of acupuncture and nimodipine to treat mild cognitive impairment after cerebral infarction： a randomized controlled trial ［J］. BMC Complement Altern Med ， 2016 ， 16 （ 1 ）： 361 .

ZHANG SH ， LIU M ， ASPLUND K ， et al . Acupuncture for acute stroke ［J］. Cochrane Database Syst Rev ， 2005 （ 2 ）： CD003317 . doi： 10.1002/14651858.CD003317.pub2 http://dx.doi.org/10.1002/14651858.CD003317.pub2 .

刘德芳，赵波，吕云利，等．针刺治疗对脑梗死急性期患者血液循环、神经功能及日常生活能力的影响［J］．现代中西医结合杂志， 2015 ， 24 （ 28 ）： 3095 - 3097 ．

杨丽，袁秀丽，刺络放血疗法机理探讨及研究进展［J］. 亚太传统医药， 2016 ， 12 （ 2 ）： 67 - 69 .

金军，岳颖，屈超超，等 . 手十二井穴刺络放血对一氧化碳中毒昏迷小鼠促醒作用及其机制的初步研究［J］. 天津中医药， 2012 ， 29 （ 1 ）： 62 - 64 .

VALDIVIA AO ， FARR V ， BHATTACHARYA SK . A novel myelin basic protein transcript variant in the murine central nervous system ［J］. Mol Bio Rep ， 2019 ， 46 （ 2 ）： 2547 - 2553 .

赵培园 . NG2在局灶性脑缺血大鼠脑内的免疫组织化学研究［D］. 昆明：昆明医科大学， 2011 .

IADECOLA C . The neurovascular unit coming of age： a journey through neurovascular coupling in health and disease ［J］. Neuron ， 2017 ， 96 （ 1 ）： 17 - 42 .

O'HERRON P ， CHHATBAR PY ， LEVY M ， et al . Neural correlates of single-vessel haemodynamic responses in vivo ［J］. Nature ， 2016 ， 534 （ 7607 ）： 378 - 382 .

YATA K ， NISHIMURA Y ， UNEKAWA M ， et al . In Vivo imaging of the mouse neurovascular unit under chronic cerebral hypoperfusion ［J］. Stroke ， 2014 ， 45 （ 12 ）： 3698 - 3703 .

HILL RA ， TONG L ， YUAN P ， et al . Regional blood flow in the normal and ischemic brain is controlled by arteriolar smooth muscle cell contractility and not by capillary pericytes ［J］. Neuron ， 2015 ， 87 （ 1 ）： 95 - 110 .

KANNURPATTI SS . Mitochondrial calcium homeostasis：implications for neurovascular and neurometabolic coupling ［J］. J Cereb Blood Flow Metab ， 2017 ， 37 （ 2 ）： 381 - 395 .

SWEENEY MD ， KISLER K ， MONTAGNE A ， et al . The role of brain vasculature in neurodegenerative disorders ［J］. Nat Neurosci ， 2018 ， 21 （ 10 ）： 1318 - 1331 .

查看原文

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Exploring the Spatiotemporal Discrimination Thinking and Application for Cognitive Impairment in Elderly Coronary Heart Disease

Effect of Tongdu Xingshen Needling Method （通督醒神针刺法） on Expression of AMPA Receptors and Their Accessory Proteins in the Hippocampus of Rats with Learning Memory Impairment After Cerebral Ischaemia-Reperfusion

Effect of Shenqi Pills （肾气丸） on Cognitive Function and Hippocampal Glucose Energy Metabolism in Type 2 Diabetes Mellitus Model Mice

The Characteristics of the Syndrome Elements Distribution of 331 Cases with Cognitive Impairment After Acute Ischemic Stroke Based on the Factor Analysis

Treating Cognitive Impairment of Children with Epilepsy Based on Consolidating Basis and Cultivating Primordial Qi

Related Author

XU Fengqin

LI Tong

ZHANG Yueying

QI Zhongwen

HUI Jiaqi

QI Shikui

RUAN Xiaodi

GAO Jing

Related Institution

Xiyuan Hospital，China Academy of Chinese Medical Sciences

Laboratory of Combining Diseases and Evidence to Prevent Vascular Aging，National Administration of Traditional Chinese Medicine

Graduate School of Beijing University of Chinese Medicine

Henan University of Chinese Medicine

The First Affiliated Hospital of Henan University of Chinese Medicine

Address：No.16, Nanxiao street, Dongzhimen, Dongcheng District, Beijing Postal code：100700
Tel：010-64035632 Email：jtcmcn@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010102007558号
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Effects of Bloodletting at “Baihui （DU 20）” and “Sishencong （EX-HN1）” on Cerebral Microinfarcts and Neurovascular Coupling in Intracranial Hypoperfusion Model Rats

DOI：10.13288/j.11-2166/r.2022.19.014

摘要

Abstract

关键词

Keywords

references