Effects of Shengjiang （<italic style="font-style: italic">Rhizoma Zingiberis Recens</italic>）-Huangqin （<italic style="font-style: italic">Radix Scutellariae</italic>） Herbal Pair at Diffe-rent Composition Ratios on Energy Metabolism Enzymes， Gastrointestinal Motility and Brain-gut Peptides in Rats with Stomach Cold Syndrome

ZHANG Ruixing; YANG Zhijun; HUANG Rong; CUI Yanhong; LI Yijie; YANG Xiujuan; YAN Xiao

doi:10.13288/j.11-2166/r.2022.22.013

您当前的位置：

首页 >

文章列表页 >

Effects of Shengjiang （Rhizoma Zingiberis Recens）-Huangqin （Radix Scutellariae） Herbal Pair at Diffe-rent Composition Ratios on Energy Metabolism Enzymes， Gastrointestinal Motility and Brain-gut Peptides in Rats with Stomach Cold Syndrome

更新时间：2022-11-11

- Effects of Shengjiang （Rhizoma Zingiberis Recens）-Huangqin （Radix Scutellariae） Herbal Pair at Diffe-rent Composition Ratios on Energy Metabolism Enzymes， Gastrointestinal Motility and Brain-gut Peptides in Rats with Stomach Cold Syndrome
- Journal of Traditional Chinese Medicine Vol. 63, Issue 22, Pages: 2162-2169(2022)
- 作者机构：
  
  甘肃中医药大学药学院，甘肃省兰州市城关区定西东路35号，730000
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2022.22.013
  CLC：
- Received：21 February 2022，
  
  Revised：2022-09-08，
  
  Published：17 November 2022
- 稿件说明：
移动端阅览
张瑞欣,杨志军,黄蓉等.不同配比生姜-黄芩药对对胃寒证模型大鼠能量代谢酶、胃肠动力及脑肠肽的影响[J].中医杂志,2022,63(22):2162-2169.

ZHANG Ruixing,YANG Zhijun,HUANG Rong,et al.Effects of Shengjiang （Rhizoma Zingiberis Recens）-Huangqin （Radix Scutellariae） Herbal Pair at Diffe-rent Composition Ratios on Energy Metabolism Enzymes， Gastrointestinal Motility and Brain-gut Peptides in Rats with Stomach Cold Syndrome[J].Journal of Traditional Chinese Medicine,2022,63(22):2162-2169.
张瑞欣,杨志军,黄蓉等.不同配比生姜-黄芩药对对胃寒证模型大鼠能量代谢酶、胃肠动力及脑肠肽的影响[J].中医杂志,2022,63(22):2162-2169. DOI： 10.13288/j.11-2166/r.2022.22.013.

ZHANG Ruixing,YANG Zhijun,HUANG Rong,et al.Effects of Shengjiang （Rhizoma Zingiberis Recens）-Huangqin （Radix Scutellariae） Herbal Pair at Diffe-rent Composition Ratios on Energy Metabolism Enzymes， Gastrointestinal Motility and Brain-gut Peptides in Rats with Stomach Cold Syndrome[J].Journal of Traditional Chinese Medicine,2022,63(22):2162-2169. DOI： 10.13288/j.11-2166/r.2022.22.013.

摘要

目的

观察生姜、黄芩不同比例配伍对胃寒证的影响，并探讨可能的作用机制。

方法

SD大鼠随机分为空白组12只和造模组60只。采用“0 ℃纯净水灌胃+冷水泡浴+氢氧化钠刺激”的方法建立大鼠胃寒证模型，造模18天。将造模成功的50只大鼠随机分为模型组、生姜组、生姜黄芩1∶1组、生姜黄芩5∶3组、生姜黄芩2∶1组，每组10只。空白组与模型组灌胃生理盐水10 ml/kg，生姜组灌胃生姜水煎液3.6 g/kg，生姜黄芩1∶1组、5∶3组、2∶1组分别灌胃相应水煎液7.2、9.6、10.8 g/kg，各组均每天灌胃1次，连续7天。观察大鼠一般状况。检测各组大鼠肝组织能量代谢酶葡萄糖激酶（GCK）、丙酮酸脱氢酶（PDH）、异柠檬酸脱氢酶（ICD）、Na

-K

-三磷酸腺苷酶（Na

-K

-ATP）、脂肪甘油三酯脂肪酶（ATGL）含量；观察各组大鼠胃组织病理形态，计算胃排空率和小肠推进率，测定胃组织脑肠肽胃动素（MTL）、5-羟色胺（5-HT）、胃泌素（GAS）、生长抑素（SS）、血管活性肠肽（VIP）含量。

结果

与空白组比较，模型组大鼠胃黏膜结构不完整，黏膜组织坏死脱落，腺体排列紊乱，可见大量炎性细胞浸润；与模型组比较，生姜黄芩1∶1组局部黏膜坏死脱落，炎细胞浸润；其余各给药组胃黏膜上皮结构完整，腺体排列整齐，未见明显炎细胞浸润。与空白组比较，模型组大鼠肝组织GCK、PDH、ICD及Na

-K

-ATP的含量，胃排空率、小肠推进率显著降低，胃组织5-HT、MTL、GAS含量显著降低，SS、VIP含量显著升高（

＜0.01）。与模型组比较，生姜组及生姜黄芩5∶3、2∶1组肝组织GCK、PDH、ICD及Na

-K

-ATP的含量，胃排空率，小肠推进率及胃组织5-HT、MTL、GAS含量显著升高，SS、VIP含量显著降低；生姜黄芩1∶1组肝组织PDH、ICD的含量显著升高，胃组织SS、VIP含量显著降低（

＜0.05或

＜0.01）。与生姜组比较，生姜黄芩5∶3组肝组织GCK、PDH、Na

-K

-ATP的含量，胃排空率、小肠推进率，胃组织5-HT、MTL、GAS含量显著升高，VIP含量显著降低；生姜黄芩2∶1组肝组织GCK、PDH、ICD、Na

-K

-ATP的含量，小肠推进率显著升高（

＜0.05或

＜0.01）。与生姜黄芩5∶3组比较，生姜黄芩2∶1组肝组织GCK、PDH、ICD，胃组织VIP显著升高，胃排空率，胃组织5-HT、MTL、GAS含量显著降低（

＜0.05或

＜0.01）。

结论

不同配比生姜、黄芩水煎液均能通过调节能量代谢酶活性以及脑肠肽含量等机制，不同程度地治疗胃寒证，以生姜、黄芩5∶3配伍效果更佳。

Abstract

Objective

To observe the effects of Shengjiang （

Rhizoma Zingiberis Recens

）-Huangqin （

Radix Scutellariae

） herbal pair at different composition ratios in the treatment of stomach cold syndrome， and to explore its potential mechanism.

Methods

SD rats were randomly divided into blank group （n=12） and model group （n=60）. The rat model of stomach cold syndrome was established by the method of gavage with pure water at 0 ℃ plus cold water bath and sodium hydroxide stimulation for 18 days. Fifty successfully modeled rats were randomly divided into model group， Shengjiang group， Shengjiang-Huangqin （S-H） 1∶1， 5∶3， and 2∶1 group， with 10 rats in each group. The blank group and the model group were gavaged with 10 mL/kg of normal saline， while Shengjiang group was gavaged with 3.6 g/kg of Shengjiang decoction， and S-H 1∶1， 5∶3 and 2∶1 groups were gavaged with 7.2， 9.6 and 10.8 g/kg of corresponding S-H decoction， respectively. All groups were gavaged once a day for 7 consecutive days. The general condition of rats was observed. The contents of energy metabolizing enzymes glucokinase （GCK）， pyruvate dehydrogenase （PDH）， isocitrate dehydrogenase （ICD）， Na

-K

-ATPase （Na

-K

-ATP） and adipose-triglyceride lipase （ATGL） in liver tissue of rats in each group were detected. The pathological morphology of gastric tissue was observed， and the gastric emptying rate and small intestinal propulsion were calculated. The contents of motilin （MTL）， 5-hydroxytryptamine （5-HT）， gastrin （GAS）， somatostatin （SS） and vasoactive intestinal peptide （VIP） in gastric tissue were determined.

Results

Compared to those in the blank group， the gastric mucosa of the rats in the model group was incomplete， and the mucosal tissue was necrotic and exfoliated， with disorderly arranged glands and a large number of infiltrated inflammatory cells. Compared to those in the model group， local mucosal necrosis and shedding， as well as inflammatory cell infiltration were seen in the S-H 1∶1 group. The gastric mucosa epithelial structure was complete， and the glands were neatly arranged in other medicinal groups， with no obvious inflammatory cell infiltration. Compared to those in the blank group， the contents of GCK， PDH， ICD and Na

-K

-ATP in liver tissue， gastric emptying rate and small intestinal propulsiion， as well as the contents of 5-HT， MTL and GAS were significantly reduced in the model group， while the contents of SS and VIP significantly increased

（P

＜0.01）. Compared to those in the model group， the contents of GCK， PDH， ICD and Na

-K

-ATP in liver tissue， gastric emptying rate and small intestinal propulsion， as well as the contents of 5-HT， MTL and GAS in stomach tissue significantly increased， while the contents of SS and VIP significantly decreased in Shengjiang group， S-H 5∶3 and 2∶1 groups； the contents of PDH and ICD in liver tissue significantly increased， while the contents of SS and VIP in stomach tissue significantly decreased in the S-H 1∶1 group （

＜0.05

＜0.01）. Compared to those in the Shengjiang group， the contents of GCK， PDH， ICD， and Na

-K

-ATP in liver tissue， gastric emptying rate， small intestinal propulsion， and the contents of 5-HT， MTL and GAS in stomach tissue significantly increased， while the VIP content significantly decreased in S-H 5∶3 group； the contents of GCK， PDH， and Na

-K

-ATP in liver tissue， and small intestinal propulsion significantly increased in S-H 2∶1 group （

＜0.01

＜0.01）. S-H 2∶1 group had higher GCK， PDH， ICD contents in liver tissue and VIP content in stomach tissue， as well as lower gastric emptying rate and contents of 5-HT， MTL and GAS in stomach tissue than S-H 5∶3 group （

＜0.05

＜0.01）.

Conclusion

S-H decoction at different composition ratios all have effects on stomach cold syndrome by regulating the activity of energy metabolism enzyme and the content of brain-gut peptide. The effect is varied， and S-H at the ratio of 5∶3 may be the best.

关键词

Keywords

references

钟赣生，杨柏灿 . 中药学［M］. 北京：中国中医药出版社， 2021 ： 38 - 39 .

段金廒，宿树兰，范欣生，等 . 基于药物相互作用探讨中药七情合和相反/相恶/相畏配伍禁忌作用模式与机制［J］. 世界科学技术-中医药现代化， 2012 ， 14 （ 3 ）： 1547 - 1552 .

黄钰芳，杨志军，邵晶，等 . 不同配伍比例下生姜对黄芩中化学成分溶出率的影响［J］. 中国现代应用药学， 2020 ， 37 （ 17 ）： 2086 - 2092 .

张仲景 . 伤寒论 . 南宁：广西科学技术出版社， 2015 ： 107 .

丁沛 . 张仲景“去性存用”立方思想举隅［J］. 南阳理工学院学报， 2009 ， 1 （ 6 ）： 100 - 103 .

吴嘉瑞，张宇晶，张晓朦，等 . 基于文献挖掘的相恶药对生姜与黄芩临床应用特点研究［J］. 中国中医药信息杂志， 2014 ， 21 （ 7 ）： 15 - 17 .

龚千锋 . 中药炮制学［M］. 4版 . 北京：中国中医药出版社， 2016 ： 79 - 86 .

杨万斌，文彬，张凌杭，等 . 大鼠胃寒证模型造模方法探索［J］. 中国中药杂志， 2015 ， 40 （ 20 ）： 4031 - 4036 .

陈艳芬，陈蔚文，李茹柳 . 大鼠寒热型胃粘膜损伤模型的研究［J］. 中药药理与临床， 2002 ， 18 （ 2 ）： 44 - 46 .

陈奇 . 中药药理研究方法学［M］. 3版 . 北京：人民卫生出版社， 2011 ： 27 - 28 .

ARUMUGAM M ， RAES J ， PELLETIER E ， et al ． Enterotypes of the human gut microbiome ［J］. Natrue ， 2011 ， 473 （ 7345 ）： 174 - 180 ．

SCALDAFERRI F ， GERARDI V ， LOPETUSO LR ， et al ． Gut microbial flora，prebiotics，and probiotics in IBD： their current usage and utility ［J］. Biomed Res Int ， 2013 ， 2013 ： 435268 . doi： 10.1155/2013/435268 http://dx.doi.org/10.1155/2013/435268 .

陈少泽，唐迎雪，梁晓东 . 论中药配伍关系中的相恶配伍［J］. 辽宁中医药大学学报， 2012 ， 14 （ 2 ）： 105 - 106 .

匡海学，王艳宏，王秋红，等 . 基于中药性味可拆分性和可组合性的中药性味理论研究新模式［J］. 世界科学技术-中医药现代化， 2011 ， 13 （ 1 ）： 25 - 29 .

蔡志强，朱劼 . 生物化学［M］. 北京：化学工业出版社， 2020 ： 253 .

GORINI A ， CANOSI U ， DEVECCHI E ， et al ． ATPases enzyme activities during ageing in different types of somatic and synaptic plasma membranes from rat frontal cerebral cortex ［J］． Prog Neuropsychopharmacol Biol Psychiatry ， 2002 ， 26 （ 1 ）： 81 - 90 .

ZIMMERMANN R ， STRAUS JG ， HAEMMERLE G ， et al ． Fat mobilization in adipose tissue is promoted by adipose triglyceride lipase ［J］. Science ， 2004 ， 306 （ 5700 ）： 1383 - 1386 .

刘娅薇，惠华英，谭周进 . 脑肠轴传输中的胃肠肽类激素［J］. 世界华人消化杂志， 2019 ， 27 （ 16 ）： 1007 - 1012 .

SIKANDER A ， RANA SV ， PRASAD KK . Role of serotonin in gastrointestinal motility and irritable bowel syndrome ［J］. Clin Chim Acta ， 2009 ， 403 （ 1-2 ）： 47 - 55 .

SANGER GJ ， WANG Y ， HOBSON A ， et al . Motilin： towards a new understanding of the gastrointestinal neuropharmacology and therapeutic use of motilin receptor agonists ［J］. Br J Pharmacol ， 2013 ， 170 （ 7 ）： 1323 - 1332 .

何美蓉，宋于刚，智发朝，等 . 功能性消化不良患者胃动力障碍与胃肠激素及G、D细胞的关系［J］. 中华消化杂志， 2004 ， 24 （ 1 ）： 56 - 57 .

TOSETTI C ， STANGHELLINI V ， TUCCI A ， et al ． Gastric emptying and dyspeptic symptoms in patients with nonautoimmune fundic atrophic gastritis ［J］． Dig Dis Sci ， 2000 ， 45 （ 2 ）： 252 - 257 ．

SVENSSON E ， KIM O ， PARKER D . Altered GABA and somatostatin modulation of proprioceptive feedback after spinal cord injury in lamprey ［J］. Neuroscience ， 2013 ， 235 ： 109 - 118 . doi： 10.1016/j.neuroscience. 2013. 01.017 http://dx.doi.org/10.1016/j.neuroscience.2013.01.017 .

IVANOVA M ， BELCHEVA S ， BELCHEVA I ， et al . Modulatory effect of VIP injected into hippocampal CA1 area on anxiety in olfactory bulbectomized rats ［J］. Acta Neurobiol Exp （Wars）， 2014 ， 74 （ 3 ）： 317 - 327 .

金光亮 . 论相辅相成与相反相成的方剂配伍思维［J］. 中医杂志， 2018 ， 59 （ 2 ）： 91 - 94 .

查看原文

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Construction and Application of "Source-Pivot-Convergence" Pattern Identification and Treatment Model for Malignant Tumors

Construction of the Mapping Frameword of the Five Zang （脏） Organs and the Mitochondrial Function Based on the "Qi Transformation into the Five Phases" Theory

Effect of Lifei Xiaoji Pill （理肺消积丸） on the Warburg Effect and USP47/BACH1 Pathway in Tumor Tissues of Lung Cancer Model Mice

Effects of Danggui Sini Granules （当归四逆颗粒） on Cardiomyocyte Apoptosis and Vasoactive Factors in Rat Models of Coronary Heart Disease with Cold Congealing and Blood Stasis Syndrome

Effect of Herbal Patching of Mahuang （Herba Ephedrae） and Huangqin （Radix Scutellariae） at Feishu （BL13） on the Surface Temperature of Neck， Chest and Back Acupoints in Healthy Subjects

Related Author

JIANG Yuling

HE Jiawei

ZHONG Yang

HUANG Chunxia

MA Qiong

ZHENG Chuan

FU Xi

YOU Fengming

Related Institution

Cancer Research Institute，Chengdu University of Traditional Chinese Medicine

TCM Prevention and Treatment of Metabolic and Chronic Diseases Key Laboratory of Sichuan Province

Affiliated Hospital of Chengdu University of Traditional Chinese Medicine

Shanghai Traditional Chinese Medicine Hospital

Beijing University of Chinese Medicine

Address：No.16, Nanxiao street, Dongzhimen, Dongcheng District, Beijing Postal code：100700
Tel：010-64035632 Email：jtcmcn@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010102007558号
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Effects of Shengjiang （Rhizoma Zingiberis Recens）-Huangqin （Radix Scutellariae） Herbal Pair at Diffe-rent Composition Ratios on Energy Metabolism Enzymes， Gastrointestinal Motility and Brain-gut Peptides in Rats with Stomach Cold Syndrome

DOI：10.13288/j.11-2166/r.2022.22.013

摘要

Abstract

关键词

Keywords

references