Influence of <italic style="font-style: italic">Yupingfeng Powder </italic>（玉屏风散） and <italic style="font-style: italic">Qiji Formula </italic>（芪寄方） on the Immune Response in Mice Injected with Split Virus Influenza Vaccine

Yiming YIN; Ying YANG; Miaomiao LIU; YIN Yiming; ZHAO Fangshu; NI Wenting; LI Baohong; WAN Xinhuan; TIAN Jingzhen

doi:10.13288/j.11-2166/r.2022.24.014

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Influence of Yupingfeng Powder （玉屏风散） and Qiji Formula （芪寄方） on the Immune Response in Mice Injected with Split Virus Influenza Vaccine

更新时间：2022-12-13

- Influence of Yupingfeng Powder （玉屏风散） and Qiji Formula （芪寄方） on the Immune Response in Mice Injected with Split Virus Influenza Vaccine
- Journal of Traditional Chinese Medicine Vol. 63, Issue 24, Pages: 2382-2387(2022)
- 作者机构：
  
  山东中医药大学药学院，山东省济南市大学路4655号，250355
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2022.24.014
  CLC：
- Received：25 April 2022，
  
  Revised：2022-09-08，
  
  Published：17 December 2022
- 稿件说明：
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尹怡铭,杨颖,刘苗苗等.玉屏风散和芪寄方对流感病毒裂解疫苗注射小鼠免疫反应的影响[J].中医杂志,2022,63(24):2382-2387.

Powder Influence of Yupingfeng,Formula Qiji,Vaccine on the Immune Response in Mice Injected with Split Virus Influenza,et al.Influence of Yupingfeng Powder （玉屏风散） and Qiji Formula （芪寄方） on the Immune Response in Mice Injected with Split Virus Influenza Vaccine[J].Journal of Traditional Chinese Medicine,2022,63(24):2382-2387.
尹怡铭,杨颖,刘苗苗等.玉屏风散和芪寄方对流感病毒裂解疫苗注射小鼠免疫反应的影响[J].中医杂志,2022,63(24):2382-2387. DOI： 10.13288/j.11-2166/r.2022.24.014.

Powder Influence of Yupingfeng,Formula Qiji,Vaccine on the Immune Response in Mice Injected with Split Virus Influenza,et al.Influence of Yupingfeng Powder （玉屏风散） and Qiji Formula （芪寄方） on the Immune Response in Mice Injected with Split Virus Influenza Vaccine[J].Journal of Traditional Chinese Medicine,2022,63(24):2382-2387. DOI： 10.13288/j.11-2166/r.2022.24.014.

摘要

目的

探讨玉屏风散和芪寄方对流感病毒裂解疫苗保护效力的影响。

方法

40只Balb/c小鼠随机分为空白组、免疫对照组、铝佐剂组、玉屏风散组、芪寄方组，每组8只。玉屏风散组小鼠给予玉屏风散0.25 g/10 g灌胃，芪寄方组小鼠给予芪寄方0.25 g/10 g灌胃，其余各组小鼠给予纯净水0.25 ml灌胃。各组均每日灌胃1次，连续10天。给药4天，空白组小鼠经腿部肌肉注射0.1 ml PBS，其余各组小鼠经腿部肌肉注射季节性流感病毒裂解疫苗（IVV）0.75 μg进行初次免疫，其中铝佐剂组IVV含100 μg 氢氧化铝。在初次免疫2周后各组以同样方法加强免疫1次。每隔7 天称取小鼠体质量1次，观察小鼠一般情况，至给药28天。采用ELISA法检测初次免疫后7、14、21、28天血清特异性抗体IgG水平，初次免疫后28天检测血清特异性抗体IgG亚型IgG

、IgG

的水平；留取脾脏称重，计算脾脏指数，并采用流式细胞术检测脾脏记忆性B细胞比例。

结果

各组间小鼠同时间体质量差异无统计学意义（

＞0.05）。与本组给药前及给药7天时比较，芪寄方组小鼠给药14天时体质量明显下降（

＜0.05）。与本组给药14天时比较，玉屏风组和芪寄方组给药28天时体质量明显上升（

＜0.05）。各组小鼠毛发柔顺光滑，一般活动正常，排泄物质地无明显异常。与空白组和免疫对照组相比，芪寄方组小鼠脾脏指数、记忆性B细胞比例明显升高（

＜0.05或

＜0.01）。与空白组比较，各组小鼠血清IgG

和IgG

水平，各时间点IgG水平均升高（

＜0.05或

＜0.01）。与免疫对照组相比，玉屏风散组初次免疫后21、28天，芪寄方组初次免疫后28天IgG水平均显著升高（

＜0.05或

＜0.01）；与铝佐剂组相比，玉屏风散组初次免疫后21天IgG水平显著升高（

＜0.05）。与免疫对照组相比，玉屏风散组和芪寄方组IgG

水平，铝佐剂组IgG

水平均显著升高（

＜0.05或

＜0.01）；与铝佐剂组相比，玉屏风散组和芪寄方组IgG

水显著升高（

＜0.05）。玉屏风散组与芪寄方组IgG

、IgG

水平，各时间点IgG水平差异均无统计学意义（

＞0.05）。

结论

玉屏风散和芪寄方可以有效提高流感裂解疫苗免疫水平，促进Th2型免疫应答，并主要提高体液免疫应答水平。

Abstract

Objective

To explore the effect of oral administration of

Yupingfeng Powder

（玉屏风散） and

Qiji Formula

（芪寄方） on the protective efficacy of the split virus influenza vaccine.

Methods

Forty Balb/c mice were randomly divided into blank group， immune control group， aluminum adjuvant group，

Yupingfeng Powder

group and

Qiji Formula

group， with eight mice in each group.

Yupingfeng Powder

group and

Qiji Formula

group were given 0.25 g/10 g of corresponding formulas by gavage， while the other groups were given 0.25 ml purified water by gavage， once every day for 10 days. After 4-day medication， mice in the blank group were injected intramuscularly with 0.1 ml of PBS on the legs， while those in other groups were injected with 0.75 μg of seasonal influenza virus split vaccine （IVV） for initial immunization， and additionally 100 μg of aluminum hydroxide was contained in IVV in the aluminum adjuvant group. Two weeks after the initial immunization， the mice in all groups were continued for a booster injection. The mice were weighed every 7 days to observe the general condition. ELISA method was used to detect the level of serum specific antibody IgG 7， 14， 21 and 28 days after the initial immunization， and 28 days after the initial immunization， the level of serum specific antibody subtype IgG

and IgG

was tested， the spleen was weighed to calculate the spleen index. The proportion of memory B cells in the spleen was detected by flow cytometry.

Results

There was no significant differences in the body weight of the mice among the groups regarding the same measurement time （

＞0.05）. The body weight significantly decreased after 14-day medication in the

Qiji Formula

group

compared to that measured before and after 7-day medication （

＜0.05）. Compared to those measured after 14-day medication， the body weight after 28-day medication significantly increased in the

Yupingfeng Powder

group

and

Qiji Formula

group （

＜0.05）. The hair of the mice in all groups was soft and smooth， with normal general activities and no obvious abnormality in the excrement. Compared to that in the blank group and the immune control group， the spleen index and the proportion of memory B cells in the

Qiji Formula

group significantly increased （

＜0.05 or

＜0.01）. Compared to those in the blank gorup， the levels of serum IgG

and IgG

in all other groups as well as Ig G levels at all measured times significantly increased （

＜0.05 or

＜0.01）. Compared to those in the immune control group， the IgG level significantly increased in the

Yupingfeng Powder

group 21 and 28 days after initial immunization， and in the

Qiji Formula

group 28 days after initial immunization （

＜0.05 or

＜0.01）. The IgG level in the

Yupingfeng Powder

group 21 days after the initial immunization was significantly higher than that in the aluminum adjuvant group （

＜0.05）. Compared to those in the immune control group， the IgG

level in the

Yupingfeng Powder

group and

Qiji Formula

group， and the IgG

level in the aluminum adjuvant group significantly increased （

＜0.05 or

＜0.01）. The IgG

level in compared with aluminum adjuvant group， Yupingfeng powder The level of IgG

in the

Yupingfeng Powder

group and

Qiji Formula

group were significantly higher than that in the aluminum adjuvant group （

＜0.05）. There was no significant difference in IgG

and IgG

levels between

Yupingfeng Powder

group and

Qiji Formula

group and IgG levels measured at different timepoints （

＞0.05）.

Conclusion

Yupingfeng Powder

and

Qiji Formula

can effectively improve the immune response of split virus influenza vaccine， promote Th2 immune response， and mainly improve the body's humoral immune response.

关键词

Keywords

references

DRONINA J ， SAMUKAITE-BUBNIENE U ， RAMANAVICIUS A . Advances and insights in the diagnosis of viral infections ［J］. J Nanobiotechnology ， 2021 ， 19 （ 1 ）： 348 .

PRACHANRONARONG KL ， CANALE AS ， LIU P ， et al . Mutations in influenza A virus neuraminidase and hemagglutinin confer resistance against a broadly neutralizing hemagglutinin stem antibody ［J］. J Virol ， 2019 ， 93 （ 2 ）： e01639-18 . doi： 10.1128/JVI.01639-18 http://dx.doi.org/10.1128/JVI.01639-18 .

FTOUH M ， KALBOUSSI N ， ABID N ， et al . Contribution of nanotechnologies to vaccine development and drug delivery against respiratory viruses ［J］. PPAR Res ， 2021 ： 6741290 . doi： 10.1155/2021/6741290 http://dx.doi.org/10.1155/2021/6741290 .

SASAKI E ， HAMAGUCHI I ， MIZUKAMI T . Pharmacodynamic and safety considerations for influenza vaccine and adjuvant design ［J］. Expert Opin Drug Met ， 2020 ， 16 （ 11 ）： 1051 - 1061 .

TREGONING JS ， RUSSELL RF ， KINNEAR E . Adjuvanted influenza vaccines ［J］. Hum Vaccin Immunother ， 2018 ， 14 （ 3 ）： 550 - 564 .

TOMLJENOVIC L ， SHAW CA . Aluminum vaccine adjuvants： are they safe？［J］. Curr Med Chem ， 2011 ， 18 （ 17 ）： 2630 - 2637 .

NOHYNEK H ， JOKINEN J ， PARTINEN M ， et al . AS03 adjuvanted AH1N1 vaccine associated with an abrupt increase in the incidence of childhood narcolepsy in Finland ［J］. PLoS One ， 2012 ， 7 （ 3 ）： e33536 .

李宗霖，廖国阳 . 流感疫苗新型佐剂的研究进展［J］. 中国生物制品学杂志， 2020 ， 33 （ 4 ）： 465 - 469 .

林巧，郑军，张国良，等 . 补益类复方中药增强流感VLPs疫苗免疫原性的佐剂效应研究［J］. 世界中西医结合杂志， 2017 ， 12 （ 1 ）： 43 - 47 .

ZHOU Y ， ZONG Y ， LIU Z ， et al . Astragalus polysaccharides enhance the immune response to OVA antigen in BALB/c mice ［J］. Biomed Res Int ， 2021 ： 9976079 . doi： 10.1155/2021/9976079 http://dx.doi.org/10.1155/2021/9976079 .

董娜，李倩，张贵强，等 . 淫羊藿多糖对甲型H1N1流感病毒裂解疫苗的免疫佐剂作用［J］. 国际药学研究杂志， 2013 ， 40 （ 1 ）： 63 - 68 .

汪青楠，吕文良，李娟梅，等 . 玉屏风散实验研究及临床应用进展［J］. 中华中医药学刊， 2020 ， 38 （ 9 ）： 165 - 168 .

金利蒙 . 医方类聚·第7册［M］. 北京：人民卫生出版社， 1982 ： 379 .

王月琦 . 新型壳聚糖季铵盐凝胶微球作为流感疫苗佐剂的研究［D］. 北京：中国科学院过程工程研究所， 2016 .

王玉光，焦扬，周平安 . 流行性感冒辨治体会［J］. 中医杂志， 2010 ， 51 （ 3 ）： 213 - 214 .

胡思源，李新民，耿福能，等 . 抗感颗粒治疗小儿流行性感冒120例多中心随机对照双盲临床研究［J］. 中医杂志， 2018 ， 59 （ 6 ）： 486 - 489 .

O'HAGAN DT ， MACKICHAN ML ， SINGH M . Recent developments in adjuvants for vaccines against infectious diseases ［J］. Biomol Eng ， 2001 ， 18 （ 3 ）： 69 - 85 .

祝峥 . 补益类中药有效成分作为新型人用疫苗佐剂的应用前景［J］. 生物技术通讯， 2004 （ 1 ）： 92 - 94 .

BARTON JC ， BARTON JC ， BERTOLI LF . Hydroxychloroquine therapy and serum immunoglobulin levels in women with IgG subclass deficiency and systemic lupus erythematosus， sjögren syndrome， and rheumatoid arthritis： a retrospective study ［J］. Arch Immunol Ther Exp （Warsz）， 2022 ， 70 （ 1 ）： 14 .

蔡琨，王平，杨翠萍，等 . 仙茅多糖辅助流感裂解疫苗对小鼠免疫原性和保护性研究［J］. 中药药理与临床， 2020 ， 36 （ 5 ）： 84 - 87 .

FAN Y ， PIAO CH ， HYEON E ， et al . Gallic acid alleviates nasal inflammation via activation of Th1 and inhibition of Th2 and Th17 in a mouse model of allergic rhinitis ［J］. Int Immunopharmacol ， 2019 ， 70 （ 13 ）： 512 - 519 .

张莉芳，廖焕金，蔡珺，等 . 记忆性B细胞体外扩增影响因素的研究进展［J］. 中国免疫学杂志， 2017 ， 33 （ 1 ）： 144 - 147 .

SHINNAKASU R ， INOUE T ， KOMETANI K ， et al . Regulated selection of germinal-center cells into the memory B cell compartment ［J］. Nat Immunol ， 2016 ， 17 （ 7 ）： 861 - 869 .

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Influence of Yupingfeng Powder （玉屏风散） and Qiji Formula （芪寄方） on the Immune Response in Mice Injected with Split Virus Influenza Vaccine

DOI：10.13288/j.11-2166/r.2022.24.014

摘要

Abstract

关键词

Keywords

references