Correlation between Serum NfL， sPD-1， sPD-L1 and the Syndrome of Kidney Deficiency and Damp-heat in Relapsing-remitting Multiple Sclerosis Patients during the Remission Period

LIU Jia; KONG Lingbo; JIA Zhen; YANG Qi; ZHANG Jingze; ZHANG Chi; LI Bin; CAO Yuanbo; SUGIMOTO KAZUO; GAO Ying

doi:10.13288/j.11-2166/r.2023.01.008

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Correlation between Serum NfL， sPD-1， sPD-L1 and the Syndrome of Kidney Deficiency and Damp-heat in Relapsing-remitting Multiple Sclerosis Patients during the Remission Period

更新时间：2022-12-29

- Correlation between Serum NfL， sPD-1， sPD-L1 and the Syndrome of Kidney Deficiency and Damp-heat in Relapsing-remitting Multiple Sclerosis Patients during the Remission Period
- Journal of Traditional Chinese Medicine Vol. 64, Issue 1, Pages: 37-42(2023)
- 作者机构：
  
  1.北京中医药大学东直门医院，北京市东城区海运仓5号，100700
  2.北京中医药大学中医脑病研究院
  3.河北医科大学第二医院
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2023.01.008
  CLC：
- Received：30 May 2022，
  
  Revised：2022-09-30，
  
  Published：02 January 2023
- 稿件说明：
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刘佳,孔令博,贾珍等.复发缓解型多发性硬化缓解期患者血清NfL、sPD-1、sPD-L1与肾虚湿热证的相关性[J].中医杂志,2023,64(01):37-42.

LIU Jia,KONG Lingbo,JIA Zhen,et al.Correlation between Serum NfL， sPD-1， sPD-L1 and the Syndrome of Kidney Deficiency and Damp-heat in Relapsing-remitting Multiple Sclerosis Patients during the Remission Period[J].Journal of Traditional Chinese Medicine,2023,64(01):37-42.
刘佳,孔令博,贾珍等.复发缓解型多发性硬化缓解期患者血清NfL、sPD-1、sPD-L1与肾虚湿热证的相关性[J].中医杂志,2023,64(01):37-42. DOI： 10.13288/j.11-2166/r.2023.01.008.

LIU Jia,KONG Lingbo,JIA Zhen,et al.Correlation between Serum NfL， sPD-1， sPD-L1 and the Syndrome of Kidney Deficiency and Damp-heat in Relapsing-remitting Multiple Sclerosis Patients during the Remission Period[J].Journal of Traditional Chinese Medicine,2023,64(01):37-42. DOI： 10.13288/j.11-2166/r.2023.01.008.

摘要

目的

初步探讨复发缓解型多发性硬化（RRMS）缓解期肾虚湿热证患者的血清学特征。

方法

回顾性选取RRMS缓解期患者27例作为RRMS组，以同期招募的26例健康志愿者作为健康组，并将RRMS组患者辨证分为肾虚湿热证和肾虚非湿热证两个亚组。采集所有受试者临床信息，采用扩展残疾量表（EDSS）评估患者神经功能损伤情况，利用单分子阵列分析技术检测受试者血清神经丝轻链蛋白（NfL）、可溶型程序性死亡受体1（sPD-1）、可溶型程序性死亡配体1（sPD-L1）水平，并采用Spearman法对RRMS患者血清NfL、sPD-1、sPD-L1水平之间的相关性及其与病程、发作次数、EDSS评分的相关性进行分析。

结果

RRMS组患者血清NfL、sPD-1、sPD-L1水平显著高于健康组（

＜0.05或

＜0.01）；肾虚湿热证亚组患者血清NfL、sPD-1、sPD-L1水平均高于肾虚非湿热证亚组患者（

＜0.05或

＜0.01）。RRMS组患者血清NfL水平与sPD-1、sPD-L1水平均呈正相关（

＜0.01），sPD-1水平与sPD-L1水平呈正相关（

＜0.001）；RRMS组患者血清sPD-1水平与发作次数呈正相关（

= 0.030）。

结论

RRMS缓解期患者血清NfL、sPD-1、sPD-L1显著升高，且肾虚湿热证患者较肾虚非湿热证患者升高程度更为明显，提示RRMS肾虚湿热证患者可能存在更为严重的免疫炎症和神经损伤。

Abstract

Objective

To preliminarily investigate the serological characteristics of relapsing-remitting multiple sclerosis （RRMS） patients with kidney deficiency and damp-heat syndrome during the remission period.

Methods

Twenty-seven patients with RRMS during the remission period were retrospectively included as the RRMS group， while 26 healthy volunteers of the same time period were recruited as the healthy group. The patients in the RRMS group were further divided into two subgroups according to whether there was damp-heat or not. The clinical data of all subjects was collected， and the expanded disability status scale （EDSS） was used to evaluate the neurological impairment. Single molecule array （Simoa） technology was used to detect the serum levels of neurofilament light chain （NfL）， soluble programmed cell death protein 1 （sPD-1）， and soluble programmed death-ligand 1 （sPD-L1）. Spearman method was taken to analyze the correlation between serum NfL， sPD-1， and sPD-L1 levels in RRMS patients， and their correlations with course of disease， attack times， and EDSS score.

Results

The serum levels of NfL， sPD-1 and sPD-L1 were significantly higher in the RRMS group than in the healthy group （

＜0.05 or

＜0.01）， and were higher in the kidney deficiency and damp-heat subgroup than in the non-damp-heat syndrome subgroup （

＜0.05 or

＜0.01）. In patients with RRMS， serum NfL level was positively correlated to sPD-1 and sPD-L1 levels （

＜0.01）， and sPD-1 level was positively correlated to sPD-L1 level （

＜0.001）； serum sPD-1 level was positively correlated to attack times （

= 0.030）.

Conclusion

Serum levels of NfL， sPD-1， and sPD-L1 in patients with RRMS significantly increase during the remission period， and more changes are found if there is kidney deficiency and damp-heat syndrome than non-damp-heat syndrome， suggesting that RRMS patients with kidney deficiency and damp-heat syndrome may be related to more serious immune inflammation and nerve damage.

关键词

Keywords

references

DOBSON R ， GIOVANNONI G . Multiple sclerosis：a review ［J］. Eur J Neurol ， 2019 ， 26 （ 1 ）： 27 - 40 .

MCGINLEY MP ， GOLDSCHMIDT CH ， RAE-GRANT AD . Diagnosis and treatment of multiple sclerosis： a review ［J］. JAMA ， 2021 ， 325 （ 8 ）： 765 - 779 .

刘剑，周莉，高颖 . 中医药治疗多发性硬化临床研究的思考［J］. 北京中医药大学学报（中医临床版）， 2012 ， 19 （ 4 ）： 14 - 18 .

GAETANI L ， BLENNOW K ， CALABRESI P ， et al . Neurofilament light chain as a biomarker in neurological disorders ［J］. J Neurol Neurosurg Psychiatry ， 2019 ， 90 （ 8 ）： 870 - 881 .

BJORNEVIK K ， MUNGER KL ， CORTESE M ， et al . Serum neurofilament light chain levels in patients with pre-symptomatic multiple sclerosis ［J］. JAMA Neurol ， 2020 ， 77 （ 1 ）： 58 - 64 .

BENKERT P ， MEIER S ， SCHAEDELIN S ， et al . Serum neurofilament light chain for individual prognostication of disease activity in people with multiple sclerosis： a retrospective modelling and validation study ［J］. Lancet Neurol ， 2022 ， 21 （ 3 ）： 246 - 257 .

DISANTO G ， BARRO C ， BENKERT P ， et al . Serum neurofilament light： a biomarker of neuronal damage in multiple sclerosis ［J］. Ann Neurol ， 2017 ， 81 （ 6 ）： 857 - 870 .

WU J ， YU Z ， CHEN G . PD-1/PD-Ls： a new target for regulating immunopathogenesis in central nervous system disorders ［J］. Curr Drug Deliv ， 2017 ， 14 （ 6 ）： 791 - 796 .

DAI S ， JIA R ， ZHANG X ， et al . The PD-1/PD-Ls pathway and autoimmune diseases ［J］. Cell Immunol ， 2014 ， 290 （ 1 ）： 72 - 79 .

JAVAN MR ， ASLANI S ， ZAMANI MR ， et al . Downre-gulation of immunosuppressive molecules， PD-1 and PD-L1 but not PD-L2， in the patients with multiple sclerosis ［J］. Iran J Allergy Asthma Immunol ， 2016 ， 15 （ 4 ）： 296 - 302 .

TRABATTONI D ， SARESELLA M ， PACEI M ， et al . Costimulatory pathways in multiple sclerosis： distinctive expression of PD-1 and PD-L1 in patients with different patterns of disease ［J］. J Immunol ， 2009 ， 183 （ 8 ）： 4984 - 4993 .

LI H ， ZHENG C ， HAN J ， et al . PD-1/PD-L1 axis as a potential therapeutic target for multiple sclerosis： a t cell perspective ［J］. Front Cell Neurosci ， 2021 ， 15 ： 716747 . doi： 10.3389/fncel.2021.716747 http://dx.doi.org/10.3389/fncel.2021.716747 .

中国免疫学会神经免疫分会，中华医学会神经病学分会神经免疫学组 . 多发性硬化诊断和治疗中国专家共识（2018版）［J］. 中国神经免疫学和神经病学杂志， 2018 ， 25 （ 6 ）： 387 - 394 .

中华中医药学会 . 中医内科常见病诊疗指南：中医病证部分［M］. 北京：中国中医药出版社， 2008 ： 141 - 143 .

王莉茹，唐璐，李薇，等 . 从湿热论治多发性硬化［J］. 环球中医药， 2021 ， 14 （ 10 ）： 1842 - 1845 .

郭亚萌，关亚超，关运祥，等 . 从“湿热成痿”辨治多发性硬化［J］. 环球中医药， 2021 ， 14 （ 3 ）： 495 - 497 .

HAASE S ， LINKER RA . Inflammation in multiple scle-rosis ［J］. Ther Adv Neurol Disord ， 2021 ， 14 ： 17562864211007687 . doi： 10.1177/17562864211007687 http://dx.doi.org/10.1177/17562864211007687 .

MOHAMMADZADEH A ， RAD IA ， AHMADI-SALMASI B . CTLA-4， PD-1 and TIM-3 expression predominantly downregulated in MS patients ［J］. J Neuroimmunol ， 2018 ， 323 ： 105 - 108 . doi： 10.1016/j.jneuroim. 2018. 08.004 http://dx.doi.org/10.1016/j.jneuroim.2018.08.004 .

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