Objective

2

To compare the difference of volatile components of Kuxingren

（

Semen Armeniacae Amarum

） in different storage conditions， and to verify the rationality of pounding to pieces when used.

Methods

2

There were 13 groups of Kuxingren samples under different storage conditions， temperature and time， which were pounding into pieces when used （0 w） group， storing at 4 ℃ for 1 w after pounding into pieces group， storing at 4 ℃ for 1 w without pounding group， storing at 25 ℃ for 1 w after pounding into pieces group， storing at 25 ℃ for 1 w without pounding group， storing at 4 ℃ for 2 w after pounding into pieces group， storing at 4 ℃ for 2 w without pounding group， storing at 25 ℃ for 2 w after pounding into pieces group， storing at 25 ℃ for 2 w without pounding group， storing at 4 ℃ for 4 w after pounding into pieces group， storing at 4 ℃ for 4 w without pounding group， storing at 25 ℃ for 4 w after pounding into pieces group， and storing at 25 ℃ for 4 w without pounding group.Headspace sampling-gas chromatography-mass spectrometry （HS-GC-MS） was used to analyze the volatile components of each Kuxingren group. After data processing by MSDIAL 4.48 software， Weishengxin website （

http：//www.bioinformatics.com.cn/

http://www.bioinformatics.com.cn/

） was used to conduct correlation analysis. Combined with principal component analysis （PCA）， partial least squares-discriminant analysis （PLS-DA）， Orthogonal partial least squares-discriminant analysis （OPLS-DA） method for pattern recognition， variable importance projection （VIP） was used to screen differential compounds.

Results

2

The components of Kuxingren were positively correlated between 0 w and 1 w (correlation coefficient (

r

)＞0.5

P

＜0.05)

while negatively correlated between 0 w and 2 w and 4 w (

r

＜0

P

＜0.05). Under the same storage time

the influence of storage temperature and storage state on the components of Kuxingren was further analyzed

and it was found that the influence on Kuxingren samples was small (

r

＞0.5). A total of 33 volatile components were identified in Kuxingren after final mass spectrometry analysis. The results of PCA showed that there was no significant difference between 0 w and 1 w samples

but significant differences between 0 w

2 w and 4 w samples. According to the OPLS-DA model with VIP＞1

unilateral

P

＜0.05

and the multiple of difference value＞2

20 different compounds were found. After stored for 1 w

only the content of benzaldehyde decreased

and there was no obvious difference of the components. Stored for 2 w

17 compounds decreased. Stored for 4 w

20 differential compounds were found.

Conclusion

2

When Kuxingren is stored for more than two weeks

its volatile components will be significantly reduced

indicating that it is reasonable to pound it to pieces when used.