Objective

2

To compare the five modeling methods of spleen

qi

deficiency syndrome， and to establish animal models consistent with clinical spleen

qi

deficiency in traditional Chinese medicine （TCM）.

Methods

2

Sixty male Kunming mice were randomly divided into normal group， diet disorder group， running group， standing group， running with diet disorder group， and standing with diet disorder group， ten mice in each group. The diet disorder group was fasted but with water for one day， and free to eat and drink for the next day. The running group ran at a speed of 16 m/min for 20 minutes every day. The standing group stood in the self-made small water environment platform box for 4 hours every day. The running with diet disorder group and the standing with diet disorder group each received the corresponding combined two interventions. All groups continued modeling for 14 days. The general condition of the mice was observed； food intake and water intake were recorded every other day， and the moisture content of feces was calculated. Open-field experiment was used to evaluate the five minute walking distance and average speed of mice. The gastric residual rate and small intestinal propulsion rate were calculated. The intestinal digestive enzyme activity and serum lactate dehydrogenase （LDH） content were detected. The serum sigA content， blood routine indicators， organ index and immune function were measured.

Results

2

Compared to the normal group， the mice in the normal group were responsive， with shiny coats， wheat-like feces， moderate softness and hardness， and an increasing trend of food intake. Compared to the normal group， the mice in the diet disorder and the running+diet disorder groups were irritable， with a lustrous coat， dull， hard and dry feces in small pieces， having decreased moisture content of feces and increased amount of food and water intake； the mice in the running group were responsive， with lusterless coats， moderately soft and hard feces， having increased fecal moisture content and food intake and decreased water intake； the mice in the standing group were unresponsive， squinting， arching their backs， with lusterless coats， soft stools in the shape of strips， easily deformed when picked up with forceps， increased moisture content of feces， and decreased food and water intake； the mice in the standing+diet disorder group were irritable， with lusterless coat and moderate fecal hardness， as well as increased fecal moisture content， daily food and water intake. The five minute distance and average speed of mice in the diet disorder group and the running group， as well as the spleen index in the running group were significantly higher than those in the normal group （

P

＜0.05）. The standing group had significantly decreased five minute distance and average speed than the diet disorder group， the running group， the running+diet disorder group， and the standing+diet disorder group， lowered gastric residual rate than the diet disorder group， increased small intestine propulsion rate than the normal， diet disorder， running， running+diet disorder groups， lowered small intestine mucosal protease and sucrase activity than the normal and stand+diet disorder group， as well as increased liver index than the running group （

P

＜0.05）. And the serum LDH content of mice in the standing group and the standing+diet disorder group was significantly lower than that in the diet disorder group （

P

＜0.05）. The standing+diet disorder group had higher liver index than the other groups， and lower platelet count than the normal group （

P

＜0.05）. The diet disorder group had higher liver index than the normal， running， and running+diet disorder groups， and lower hemoglobin count than the standing group （

P

＜0.05）. The running+diet disorder group had decreased red blood cells than the standing and standing+diet disorder groups， and decreased hemoglobin count than the normal， standing， and standing+diet disorder groups （

P

＜0.05）.

Conclusion

2

The model established by using the small platform of the water environment is more consistent with the diagnostic criteria of spleen

qi

deficiency syndrome.