Effects of <italic style="font-style: italic">Fuzheng Qudu Decoction </italic>（扶正祛毒汤）Drug-containing Serum on CD34<sup>+</sup> Cells Induced Dendritic Cells and Expression of Costimulatory Molecules B7-1 and B7-2 in Patients with Complete Remission of Acute Myeloid Leukemia

PENG Mingxing; HUANG Liming; ZHU Guoqing; ZHOU Wei; YIN Shangjin; CHEN Menghao; ZUO Le

doi:10.13288/j.11-2166/r.2023.14.011

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Effects of Fuzheng Qudu Decoction （扶正祛毒汤）Drug-containing Serum on CD34⁺ Cells Induced Dendritic Cells and Expression of Costimulatory Molecules B7-1 and B7-2 in Patients with Complete Remission of Acute Myeloid Leukemia

更新时间：2023-08-01

- Effects of Fuzheng Qudu Decoction （扶正祛毒汤）Drug-containing Serum on CD34⁺ Cells Induced Dendritic Cells and Expression of Costimulatory Molecules B7-1 and B7-2 in Patients with Complete Remission of Acute Myeloid Leukemia
- Journal of Traditional Chinese Medicine Vol. 64, Issue 14, Pages: 1469-1474(2023)
- 作者机构：
  
  1.贵州中医药大学，贵州省贵阳市南明区市东路50号，550025
  2.贵州中医药大学第二附属医院
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2023.14.011
  CLC：
- Received：17 November 2022，
  
  Revised：2023-03-14，
  
  Published：17 July 2023
- 稿件说明：
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彭名行,黄礼明,朱国庆等.扶正祛毒汤含药血清对急性髓系白血病完全缓解患者CD34⁺细胞诱导成树突细胞及共刺激分子B7-1、B7-2表达的影响[J].中医杂志,2023,64(14):1469-1474. DOI: 10.13288/j.11-2166/r.2023.14.011.

PENG Mingxing,HUANG Liming,ZHU Guoqing,et al.Effects of Fuzheng Qudu Decoction （扶正祛毒汤）Drug-containing Serum on CD34⁺ Cells Induced Dendritic Cells and Expression of Costimulatory Molecules B7-1 and B7-2 in Patients with Complete Remission of Acute Myeloid Leukemia[J].Journal of Traditional Chinese Medicine,2023,64(14):1469-1474. DOI: 10.13288/j.11-2166/r.2023.14.011.
彭名行,黄礼明,朱国庆等.扶正祛毒汤含药血清对急性髓系白血病完全缓解患者CD34⁺细胞诱导成树突细胞及共刺激分子B7-1、B7-2表达的影响[J].中医杂志,2023,64(14):1469-1474. DOI: 10.13288/j.11-2166/r.2023.14.011. DOI：

PENG Mingxing,HUANG Liming,ZHU Guoqing,et al.Effects of Fuzheng Qudu Decoction （扶正祛毒汤）Drug-containing Serum on CD34⁺ Cells Induced Dendritic Cells and Expression of Costimulatory Molecules B7-1 and B7-2 in Patients with Complete Remission of Acute Myeloid Leukemia[J].Journal of Traditional Chinese Medicine,2023,64(14):1469-1474. DOI: 10.13288/j.11-2166/r.2023.14.011. DOI：

摘要

目的

探讨扶正祛毒汤治疗微小残留白血病的可能作用机制。

方法

12只新西兰大白兔随机分为扶正祛毒汤低、中、高剂量组以及正常组，每组3只。扶正祛毒汤低、中、高剂量组兔分别给予扶正祛毒汤浓缩煎剂5、10、20 g/（kg·d）灌胃3天，正常组兔每天给予7.5 ml蒸馏水灌胃，末次灌胃后心脏取血获得正常血清和扶正祛毒汤低、中、高剂量血清。将急性髓系白血病完全缓解患者骨髓稀释后分离提纯CD34

细胞，将扩增后的CD34

细胞分为空白组、细胞因子组、正常血清组和扶正祛毒汤低、中、高剂量血清组。除空白组外，其余各组均添加细胞因子诱导9天成为树突细胞，诱导前及培养过程中观察细胞形态。9天后检测树突细胞表面抗原分子CD83、CD1a、人白细胞DR抗原（HLA-DR）、B7-1、B7-2表达，检测树突细胞中B7-1、B7-2蛋白及 mRNA表达。

结果

空白组细胞始终未观察到树突状改变；细胞因子组、正常血清组和扶正祛毒汤低、中、高剂量血清组细胞胞体明显增大，细胞表面呈现星状多形性或树枝状典型突起，其中扶正祛毒汤中剂量血清组最为典型。与空白组比较，其余各组树突细胞表面标志物CD1a、CD83、HLA-DR、B7-1、B7-2表达均升高（

＜0.05）；与细胞因子组和正常血清组比较，扶正祛毒汤低、中、高剂量血清组DC表面各标志物表达均升高（

＜0.05）。与空白组、细胞因子组和正常血清组比较，扶正祛毒汤低、中、高剂量血清组B7-1和B7-2蛋白及mRNA表达均升高（

＜0.05）。与扶正祛毒汤中剂量血清组比较，扶正祛毒汤低、高剂量血清组B7-1和B7-2蛋白及mRNA表达均降低（

＜0.05）。

结论

扶正祛毒汤可促进急性髓系白血病完全缓解患者CD34

细胞源树突细胞的成熟，增加树突细胞中共刺激分子B7-1、B7-2的表达，提高抗原提呈能力，这可能是其治疗微小残留白血病的作用机制之一。

Abstract

Objective

To explore the possible mechanism of

Fuzheng Qudu Decoction

（扶正祛毒汤， FQD） in the treatment of minimal residual leukemia.

Methods

Twelve New Zealand white rabbits were randomly divided into normal group and low-， medium-， high-dose FQD groups， with three rabbits in each group. Rabbits in the low-， medium-， and high-dose groups of FQD were given concentrated decoction of 5， 10 and 20 g/（kg·d） by gavage for 3 days， while the normal group was given 7.5 ml of normal saline by gavage. After the last intragastric administration， blood was collected from the heart to obtain normal serum， and low-， medium- and high-dose FQD serum. After diluting the bone marrow of patients with complete remission of acute myeloid leukemia， the CD34

cells were isolated and purified； and the expanded CD34

cells were divided into blank group， cytokine group， normal serum group and FQD low-， medium- and high-dose serum groups. Except for the blank group， samples were added with cytokines to induce dendritic cells within 9 days， and the cell morphology was observed before induction and during culture. Nine days later， the expression of dendritic cell surface antigen molecules CD1a， CD83， human leukocyte DR antigen （HLA-DR）， B7-1， and B7-2 were detected， and the expression of protein and mRNA of B7-1 and B7-2 in dendritic cells was detected.

Results

No dendritic changes were observed in the cells of the blank group； the cell bodies were significantly enlarged， and the cell surface showed typical stellate pleomorphism or dendritic protrusions in the cytokine group， normal serum group and FQD low-， medium-， and high-dose serum groups， with medium-dose serum group as the most typical one. Compared to those in the blank group， the expressions of dendritic cell surface markers CD1a， CD83， HLA-DR， B7-1， and B7-2 in other groups all increased （

＜0.05）； compared to the cytokine group and normal serum group， the three FQD groups had higher expressions of dendritic cell surface antigen molecules （

＜0.05）. Compared to those in the blank group， cytokine group and normal serum group， the expression of B7-1 and B7-2 protein and mRNA in the low-， medium-， and high-dose FQD serum groups all increased （

＜0.05）. Compared to those in the medium-dose FQD serum group， the expressions of B7-1 and B7-2 protein and mRNA in the low- and high-dose serum groups all decreased （

＜0.05）.

Conclusion

FQD can promote the maturation of CD34

cell-derived dendritic cells， increase the expression of co-stimulatory molecules B7-1 and B7-2， and improve the ability of antigen presentation in patients with acute myeloid leukemia in complete remission， which may represent one mechanism of action in minimal residual leukemia by FQD.

关键词

Keywords

references

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Effects of Fuzheng Qudu Decoction （扶正祛毒汤）Drug-containing Serum on CD34+ Cells Induced Dendritic Cells and Expression of Costimulatory Molecules B7-1 and B7-2 in Patients with Complete Remission of Acute Myeloid Leukemia

DOI：10.13288/j.11-2166/r.2023.14.011

摘要

Abstract

关键词

Keywords

references

Effects of Fuzheng Qudu Decoction （扶正祛毒汤）Drug-containing Serum on CD34⁺ Cells Induced Dendritic Cells and Expression of Costimulatory Molecules B7-1 and B7-2 in Patients with Complete Remission of Acute Myeloid Leukemia