Metagenomics of Fecal Gut Microbiota in Common Traditional Chinese Medicine Syndrome Types of Irritable Bowel Syndrome with Diarrhea：A Cross-sectional Study

XIONG Qin; LI Yilin; YAO Chengjiao; LUO Lihong; XIE Fengjiao; YANG Chunrong; DONG Chaoqiang; FENG Peimin

doi:10.13288/j.11-2166/r.2024.05.011

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Metagenomics of Fecal Gut Microbiota in Common Traditional Chinese Medicine Syndrome Types of Irritable Bowel Syndrome with Diarrhea：A Cross-sectional Study

更新时间：2024-02-26

- Metagenomics of Fecal Gut Microbiota in Common Traditional Chinese Medicine Syndrome Types of Irritable Bowel Syndrome with Diarrhea：A Cross-sectional Study
- Journal of Traditional Chinese Medicine Vol. 65, Issue 5, Pages: 503-511(2024)
- 作者机构：
  
  成都中医药大学附属医院，四川省成都市金牛区十二桥路39号，610075
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2024.05.011
  CLC：
- Received：09 April 2023，
  
  Revised：2023-12-13，
  
  Published：02 March 2024
- 稿件说明：
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熊钦,李奕霖,姚承佼等.腹泻型肠易激综合征常见证型粪便肠道菌群宏基因组学横断面研究[J].中医杂志,2024,65(05):503-511.

XIONG Qin,LI Yilin,YAO Chengjiao,et al.Metagenomics of Fecal Gut Microbiota in Common Traditional Chinese Medicine Syndrome Types of Irritable Bowel Syndrome with Diarrhea：A Cross-sectional Study[J].Journal of Traditional Chinese Medicine,2024,65(05):503-511.
熊钦,李奕霖,姚承佼等.腹泻型肠易激综合征常见证型粪便肠道菌群宏基因组学横断面研究[J].中医杂志,2024,65(05):503-511. DOI： 10.13288/j.11-2166/r.2024.05.011.

XIONG Qin,LI Yilin,YAO Chengjiao,et al.Metagenomics of Fecal Gut Microbiota in Common Traditional Chinese Medicine Syndrome Types of Irritable Bowel Syndrome with Diarrhea：A Cross-sectional Study[J].Journal of Traditional Chinese Medicine,2024,65(05):503-511. DOI： 10.13288/j.11-2166/r.2024.05.011.

摘要

目的

探讨腹泻型肠易激综合征（IBS-D）常见证候肠道菌群结构及功能特征。

方法

招募2020年1月1日至2021年3月31日就诊于成都中医药大学附属医院的IBS-D患者，及同期成都中医药大学附属医院体检中心招募的健康受试者。IBS-D患者分为肝郁脾虚型IBS-D、脾虚湿盛型IBS-D，与健康受试者分别组成肝郁脾虚组、脾虚湿盛组及健康组。采集一般资料，包括年龄、性别、身体质量指数（BMI），IBS-D患者另采集肠易激综合征症状严重程度量表（IBS-SSS）、肠易激综合征生活质量评分量表（IBS-QOL）评分。同时收集新鲜粪便标本，采用宏基因组测序技术进行检测，对注释的肠道菌群结构与功能进行丰度统计展示、PCoA、Anosim、LEfSe生物信息分析。

结果

三组受试者一般资料差异无统计学意义（

＞0.05）；肝郁脾虚组、脾虚湿盛组IBS-SSS、IBS-QOL评分差异无统计学意义（

＞0.05）。研究纳入肝郁脾虚组、脾虚湿盛组及健康组各28例。肝郁脾虚组患者特有基因数量为269 135个，脾虚湿盛组特有基因数量为216 156个，健康组特有基因数量为249 759个，三组共有基因数量为1 784 036个。三组间在肠道菌群排名前十物种的相对丰度分布上存在差异，在门、纲、目层级差异较小，在科、属、种层级差异较大。三组间肠道菌群结构与功能相对丰度存在差异。种水平的物种PCoA、Anosim分析显示三组间菌群组成有明显差别。进一步LEfSe分析显示，肝郁脾虚组患者筛选到14种优势菌种，其中

Clostridium

sp.CAG 217、

Lachnospira pectinoschiza

、

Anaerotruncus

sp. CAG 528、

Paeniclostridium sordellii

、

Eubecterium

sp. CAG 76、

Bacillus cereus

丰度差异影响程度较大；脾虚湿盛组筛选到24种优势菌种，其中

Roseburia inulinivorans

、

Eubacterium

sp. CAG 251、

Roseburia hominis

、Unclassified

Eubacterium rectale

、

Roseburia intestinalis

、

Megamonas funiformis

丰度差异影响程度较大；未筛选到肝郁脾虚组患者的优势功能基因，脾虚湿盛组筛选到鞭毛装配（ko02040）、卟啉代谢（ko00860）、沙门氏菌感染（ko05132）、苯甲酸降解（ko00362）4种优势功能基因。肝郁脾虚组、脾虚湿盛组差异优势功能基因可能主要集中在新陈代谢（包括外源物质的生物降解和代谢、能量代谢、脂质代谢等）方面。

结论

肝郁脾虚型IBS-D患者肠道菌群结构特征表现为包括

Clostridium

sp. CAG 217在内的14种菌种富集。脾虚湿盛型IBS-D患者肠道菌群结构特征表现为包括

Roseburia inulinivorans

在内的24种菌种富集，功能特征表现为鞭毛装配等4种功能富集。

Clostridium

sp.CAG 217、

Roseburia inulinivorans

分别有望成为肝郁脾虚、脾虚湿盛型IBS-D患者的生物标志物。

Abstract

Objective

To investigate the structural and functional characteristics of gut microbiota in common traditional Chinese medicine （TCM） syndromes of irritable bowel syndrome with diarrhea （IBS-D）.

Methods

IBS-D patients who visited the Hospital of Chengdu University of Traditional Chinese Medicine， and healthy participants from the Physical Examination Centre of the same hospital were recruited from 1st January 2020 to 31st March 2021.The IBS-D patients were classified into syndrome of liver constraint and spleen deficiency， and syndrome of spleen deficiency and dampness exuberance； together with the recruited healthy participants， there were liver-constraint group， dampness-exuberance group， and healthy group. General information， including age， gender and body mass index （BMI）， were collected， and Irritable Bowel Syndrome Symptom Severity Scale （IBS-SSS） as well as Irritable Bowel Syndrome Quality of Life Scale （IBS-QOL） scores were additionally collected from IBS-D patients. Fresh fecal samples were also collected and tested by macro-genome sequencing technology for abundance statistical display， PCoA， Anosim， LEfSe bioinformatic analysis of the annotated gut microbiota structure and function.

Results

There was no statistically significant difference in the general information of the participants in the three groups （

＞0.05）； the difference in the IBS-SSS and IBS-QOL scores between liver-constraint group and dampness-exuberance group were not statistically significant （

＞0.05）. The study included 28 cases each in liver-constraint group， dampness-exuberance group， and healthy group. The number of specific genes to patients in liver-constraint group was 269 135， with 216 156 in dampness-exuberance group and 249 759 in healthy group， accounting of total 1 784 036 in the three groups. There were differences in the relative abundance distribution of the top ten species of gut microbiota among the three groups， with smaller differences at the phylum， class and order levels， and larger differences at the family， genus and species levels. There were differences in the relative abundance of structure and function of the gut microbiota among the three groups. Species PCoA and Anosim analyses at the species level showed significant differences in the composition of the microbiota among the three groups. Further LEfSe analyses showed that patients in liver-constraint group were screened for 14 dominant strains， of which

Clostridium

sp. CAG 217，

Lachnospira pectinoschiza

，

Anaerotruncus

sp. CAG 528，

Paeniclostridium sordellii

，

Eubecterium

sp. CAG 76，

Bacillus cereus

were affected to a greater extent in abundance differences； dampness-exuberance group screened 24 species of dominant bacteria， of which

Roseburia inulinivorans

，

Eubacterium

sp. CAG 251，

Roseburia hominis

， Unclassified

Eubacterium rectale

，

Roseburia intestinalis

， and

Megamonas funiformis

were affected to a greater extent in abundance differences； no dominant functional genes were screened for patients in liver-constraint group， and dampness-exuberance group was screened for flagellum assembly （ko02040）， porphyrin metabolism （ ko00860）， salmonella infection （ko05132）， and benzoic acid degradation （ko00362）. The differentially dominant functional genes in liver-constraint group and dampness-exuberance group may mainly focus on metabolism （including biodegradation and metabolism of exogenous substances， energy metabolism， lipid metabolism， etc.）.

Conclusion

IBS-D with syndrome of liver constraint and spleen deficiency is characterized by the enrichment of 14 gut microbiota， such as

Clostridium

sp. CAG 217， while IBS-D with syndrome of spleen deficiency and dampness exuberance is characterized by the enrichment of 24 gut microbiota， such as

Roseburia inulinivorans

， and 4 functional enrichments， such as flagellum assembly.

Clostridium

sp. CAG 217 and

Roseburia inulinivorans

are expected to be biomarkers for IBS-D patients in the two syndromes， respectively.

关键词

Keywords

references

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