Effects of Tuina at "Weizhong" （BL 40） on Synaptic Plasticity in the Hippocampal CA3 Region in Chronic Sciatic Nerve Compression Injury Model Rats

HUANG Hongye; CHEN Shuijin; CHEN Lechun; ZHANG Huanzhen; JIANG Jingjing; JIANG Yu; CHEN Jincheng; LIN Zhigang

doi:10.13288/j.11-2166/r.2024.15.013

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Effects of Tuina at "Weizhong" （BL 40） on Synaptic Plasticity in the Hippocampal CA3 Region in Chronic Sciatic Nerve Compression Injury Model Rats

更新时间：2024-08-01

- Effects of Tuina at "Weizhong" （BL 40） on Synaptic Plasticity in the Hippocampal CA3 Region in Chronic Sciatic Nerve Compression Injury Model Rats
- Journal of Traditional Chinese Medicine Vol. 65, Issue 15, Pages: 1602-1610(2024)
- 作者机构：
  
  路13号，350003
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2024.15.013
  CLC：
- Received：25 February 2024，
  
  Revised：2024-03-26，
  
  Published：02 August 2024
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黄红叶,陈水金,陈乐春等.推拿“委中”穴对坐骨神经慢性压迫性损伤模型大鼠海马CA3区突触可塑性的影响[J].中医杂志,2024,65(15):1602-1610.

HUANG Hongye,CHEN Shuijin,CHEN Lechun,et al.Effects of Tuina at "Weizhong" （BL 40） on Synaptic Plasticity in the Hippocampal CA3 Region in Chronic Sciatic Nerve Compression Injury Model Rats[J].Journal of Traditional Chinese Medicine,2024,65(15):1602-1610.
黄红叶,陈水金,陈乐春等.推拿“委中”穴对坐骨神经慢性压迫性损伤模型大鼠海马CA3区突触可塑性的影响[J].中医杂志,2024,65(15):1602-1610. DOI： 10.13288/j.11-2166/r.2024.15.013.

HUANG Hongye,CHEN Shuijin,CHEN Lechun,et al.Effects of Tuina at "Weizhong" （BL 40） on Synaptic Plasticity in the Hippocampal CA3 Region in Chronic Sciatic Nerve Compression Injury Model Rats[J].Journal of Traditional Chinese Medicine,2024,65(15):1602-1610. DOI： 10.13288/j.11-2166/r.2024.15.013.

摘要

目的

从海马突触可塑性角度探讨推拿委中穴缓解坐骨神经痛的可能作用机制。

方法

60只SD大鼠随机分为假手术组、模型组、推拿组、MK-801组、MK-801+推拿组，每组12只。所有大鼠侧脑室置管术后，除假手术组外其余各组大鼠制备右侧坐骨神经慢性压迫性损伤模型。造模后第4天，推拿组大鼠开始推拿“委中”穴干预，每日1次持续10 min，共干预14天；MK-801组大鼠开始侧脑室注射0.25 μg/μl的N-甲基-D天冬氨酸受体2B（NR2B）阻断剂地佐环平（MK-801）0.5 μl，每日1次，共14天。MK-801+推拿组大鼠在侧脑室注射MK-801 30 min后进行推拿干预，时间方法同上；模型组和假手术组大鼠不进行任何干预。大鼠造模前1天（基础值）及造模第4、10、14、18天进行自发痛行为评分和机械足反射阈值（PWTs）检测，第19天各组大鼠脑组织取材后，使用尼氏染色观察海马CA3区尼氏阳性细胞数量与形态，透射电镜观察海马CA3区突触数目、突触间隙、突触后致密物厚度、活性带长度与突触界面曲率，免疫荧光染色和免疫印迹法检测海马CA3区突触相关蛋白NR2B、突触后致密物95（PSD95）的表达。

结果

与假手术组同时间比较，模型组造模后第4、10、14、18天时自发性疼痛评分显著增加，PWTs降低（

＜0.

05）；与模型组比较，推拿组大鼠在造模第10、14、18天自发性疼痛评分显著下降，在造模第14、18天PWTs显著升高（

＜0.05）；与推拿组比较，MK-801+推拿组自发性疼痛评分在造模第10、14、18天升高，在造模第14、18天PWTs降低（

＜0.05）。与假手术组比较，模型组大鼠海马CA3区神经元排列紊乱，尼氏阳性细胞数量、突触数目、突触后致密度厚度、活性带长度、突触界面曲率减小，突触间隙变宽，海马CA3区NR2B、PSD95免疫荧光阳性表达及免疫印迹蛋白表达减少（

＜0.05）。与模型组比较，推拿组大鼠海马CA3区神经细胞排列密集，尼氏阳性细胞数量、突触数目、突触后致密物厚度、活性带长度均增加，突触间隙明显变窄，海马CA3区NR2B、PSD95免疫荧光阳性表达及免疫印迹蛋白表达较模型组增加（

＜0.05）。与推拿组比较，MK-801+推拿组海马CA3区神经元形态破坏严重，尼氏阳性细胞数量、突触数目、突触后致密物厚度、活性带长度、突触界面曲率减小，突触间隙变宽，NR2B、PSD95免疫荧光阳性表达和免疫印迹蛋白表达降低（

＜0.05）。

结论

推拿“委中”穴具有显著的镇痛作用，显著增加海马CA3区NR2B和PSD95蛋白表达水平从而调控海马突触可塑性是其发挥镇痛作用的可能作用机制之一。

Abstract

Objective

To explore the possible mechanism of Tuina at Weizhong （BL 40） for relieving sciatica from the perspective of hippocampal synaptic plasticity.

Methods

Sixty SD rats were randomly divided into sham operation group， model group， Tuina group， MK-801 group， MK-801 plus Tuina group， 12 rats in each group. After lateral ventricular cannulation， rats model with chronic compression injury of the right sciatic nerve were prepared in all groups except the sham operation group. On day 4 after modelling， rats in the Tuina group start Tuina at Weizhong （BL 40） for 10 mins once a day for a total of 14 days； rats in the MK-801 group started injecting with 0.25 μg/μl of the N-methyl-D-aspartate receptor 2B （NR2B） blocker， dizocycline （MK-801）， 0.5 μl of which was administered daily in the lateral ventricle for 14 days. Rats in the MK-801 plus Tuina group underwent Tuina after 30 mins when completing MK-801 injection in the lateral ventricle， in the same way as above； rats in the model group and the sham operation group did not undergo any intervention. Spontaneous pain behaviour scores and paw withdraw thresholds （PWTs） were examined on day 1 （base value） before modelling and on day 4， 10， 14 and 18 after modelling； and on day 19， the brain tissues of the rats in each group were sampled and the number and morphology of the Nysted-positive cells in the hippocampal CA3 region were observed using Nysted staining； and the number of synapses， the thickness of postsynaptic dense material， the length of active band and the curvature of synaptic interface in hippocampal CA3 region were observed by transmission electron microscopy； and the expression of synapse-associated proteins NR2B and postsynaptic density protein-95 （PSD95） in hippocampal CA3 was detected by immunofluorescence staining and immunoblotting.

Results

Compared with the same time in the sham operation group， spontaneous pain scores significantly increased and PWTs decreased on day 4， 10， 14， and 18 after modelling in the model group （

＜0.05）； compared with the model group， spontaneous pain scores in Tuina group of rats significantly decreased on day 10， 14， and 18 after modelling， and PWTs significantly increased on day 14 and 18 after modelling （

＜0.05）. Compared with Tuina group， spontaneous pain scores increased on day 10， 14， and 18 of modelling， and PWTs decreased at days 14 and 18 of modelling in the MK-801 plus Tuina group had higher spontaneous pain scores on days 10， 14， and 18 after modelling and lower PWTs on days 14 and 18 after modelling （

＜0.05）. Compared with the sham operation group， the neuronal arrangement in the hippocampal CA3 region of the rats in the model group was disordered， with decreased number of Nysted-positive cells and synapses， reduced thickness of postsynaptic densities， length of active bands， and curvature of synaptic interfaces， wider synaptic gaps， and decreased immunofluorescent positive expression of NR2B and PSD95 as well as the expression of immunoblotting proteins in hippocampal CA3 region （

＜0.05）. Compared with the model group， more dense arranged nerve cells， the number of Nysted-positive cells， the number of synapses， the thickness of post

synaptic dense material， the length of active bands increased， the synaptic gap became significantly narrower， and the positive expression of immunofluorescence and immunoblotting protein expression of NR2B， PSD95 increased in the rat hippocampal CA3 region of Tuina group （

＜0.05）. Compared with Tuina group， the neuronal morphology of the hippocampal CA3 region in MK-801 plus Tuina group was severely damaged， and the number of Nystrom's-positive cells， the number of synapses， the thickness of post-synaptic densities， the length of active bands， and the curvature of synaptic interfaces reduced， the synaptic gaps became wider， and the immunofluorescent positive expression of NR2B， PSD95， and the expression of immunostained proteins decreased （

＜0.05）.

Conclusion

Tuina at "Weizhong" （BL 40） showed

significant analgesic effect， and one of the possible mechanisms concluded as significantly increasing the levels of NR2B and PSD95 protein expression in hippocampal CA3 region and thus modulating the synaptic plasticity of the hippocampus.

关键词

Keywords

references

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