Analysis of Tongue Coating Microbiota Characteristics in Coronary Heart Disease with <italic style="font-style: italic">Qi</italic> Deficiency and Blood Stasis Syndrome

WANG Chuhao; LIU Yongyue; DING Zhaoxuan; ZHANG Xiaoqing

doi:10.13288/j.11-2166/r.2025.05.011

您当前的位置：

首页 >

文章列表页 >

Analysis of Tongue Coating Microbiota Characteristics in Coronary Heart Disease with Qi Deficiency and Blood Stasis Syndrome

更新时间：2025-03-06

- Analysis of Tongue Coating Microbiota Characteristics in Coronary Heart Disease with Qi Deficiency and Blood Stasis Syndrome
  增强出版
- Journal of Traditional Chinese Medicine Vol. 66, Issue 5, Pages: 501-508(2025)
- 作者机构：
  
  路11 号，100029
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2025.05.011
  CLC：
- Received：14 October 2024，
  
  Revised：2024-11-30，
  
  Published：02 March 2025
- 稿件说明：
移动端阅览
王楚皓,刘永月,丁照烜等.冠心病气虚血瘀证舌苔菌群特征分析[J].中医杂志,2025,66(05):501-508.

WANG Chuhao,LIU Yongyue,DING Zhaoxuan,et al.Analysis of Tongue Coating Microbiota Characteristics in Coronary Heart Disease with Qi Deficiency and Blood Stasis Syndrome[J].Journal of Traditional Chinese Medicine,2025,66(05):501-508.
王楚皓,刘永月,丁照烜等.冠心病气虚血瘀证舌苔菌群特征分析[J].中医杂志,2025,66(05):501-508. DOI： 10.13288/j.11-2166/r.2025.05.011.

WANG Chuhao,LIU Yongyue,DING Zhaoxuan,et al.Analysis of Tongue Coating Microbiota Characteristics in Coronary Heart Disease with Qi Deficiency and Blood Stasis Syndrome[J].Journal of Traditional Chinese Medicine,2025,66(05):501-508. DOI： 10.13288/j.11-2166/r.2025.05.011.

摘要

目的

探究冠心病气虚血瘀证舌苔菌群特征。

方法

纳入冠心病气虚血瘀证患者27例组成气虚血瘀证组，非气虚血瘀证冠心病患者29例组成非气虚血瘀证组，健康人20例组成健康组。使用16S rDNA高通量测序技术检测受试者舌苔菌群，并进行Alpha、Beta多样性分析和菌群丰度比较。

结果

共检测出479条操作分类单元（OTU），其中245条OTU为三组共有，气虚血瘀证组特有OTU 33条，非气虚血瘀证组特有OTU 21条，健康组特有OTU 121条。气虚血瘀证组、非气虚血瘀证组受试者舌苔菌群观测物种数（Sob）、菌群物种总数（Chao1）、丰富度覆盖指数（ACE）、香浓指数（Shannon）较健康组减少（

＜0.05）。三组间舌苔菌群PCoA分析距离矩阵差异具有统计学意义（

＜0.05）。与健康组比较，气虚血瘀证组受试者舌苔放线菌门、帕氏菌门、螺旋体菌门、疣微菌门、罗氏菌属、TM7X、珍珠链菌属、棒状杆菌属丰度增加，梭杆菌门、蓝细菌门、细长丝菌属、乳酸菌属丰度减少（

＜0.05）；非气虚血瘀证组受试者舌苔放线菌门、疣微菌门、罗氏菌属、棒状杆菌属丰度增加，蓝细菌门、乳酸菌属丰度减少（

＜0.05）。与非气虚血瘀证组比较，气虚血瘀证组受试者舌苔帕氏菌门、消化链球菌属、索罗杆菌属、丝状菌属、莫雷梭菌、牙髓卟啉单胞菌、龈沟产线菌的丰度显著增加，蓝细菌门比例减少（

＜0.05）。

结论

冠心病气虚血瘀证患者呈现有益菌减少，致病菌增加的特点，帕氏菌门、消化链球菌属、索罗杆菌属、丝状菌属、莫雷梭菌、牙髓卟啉单胞菌、龈沟产线菌是其与非气虚血瘀证的差异菌群。

Abstract

Objective

To explore the characteristics of the tongue coating microbiota in patients of coronary heart disease （CHD） with

deficiency and blood stasis syndrome.

Methods

A total of 27 CHD patients w

ith

deficiency and blood stasis syndrome， 29 patients with non-

deficiency and blood stasis syndrome， and 20 healthy individuals were included in this study. The tongue coating microbiota of the participants was analyzed using 16S rDNA high-throughput sequencing technology， followed by Alpha and Beta diversity analyses and comparisons of microbial abundance.

Results

A total of 479 operational taxonomic units （OTUs） were detected， among which 245 OTUs were shared across all three groups. There were 33 OTUs unique to the

deficiency and blood stasis syndrome group， 21 OTUs unique to the non-

deficiency and blood stasis syndrome group， and 121 OTUs unique to the healthy group. The observed species count （Sob）， total species richness （Chao1）， abundance-based coverage estimator （ACE）， and Shannon diversity index were significantly lower in the

deficiency and blood stasis syndrome and non-

deficiency and blood stasis syndrome groups compared to the healthy group （

＜0.05）. Principal coordinate analysis （PCoA） of the tongue coating microbiota showed significant differences in distance matrices among the three groups （

＜0.05）. Compared with the healthy group， the

deficiency and blood stasis syndrome group exhibited an increased abundance of Actinobacteria， Patescibacteria， Spirochaetes， Verrucomicrobia， Rothia， TM7X， Gemella， and Corynebacterium， while Fusobacteria， Cyanobacteria， Leptotrichia， and Lactobacillus decreased （

＜0.05）. In the non-

deficiency and blood stasis syndrome group， Actinobacteria， Verrucomicrobia， Rothia， and Corynebacterium increased， whereas Cyanobacteria and Lactobacillus reduced （

＜0.05）. When comparing with the non-

deficiency and blood stasis syndrome group， the

deficiency and blood stasis syndrome group h

ad a significantly higher abundance of Patescibacteria， Peptostreptococcus， Solobacterium， Filifactor， Moraxella， Porphyromonas endodontalis， and Capnocytophaga， while Cyanobacteria reduced （

＜0.05）. Conclusuion Patients with CHD of

deficiency and blood stasis syndrome exhibit a decrease in beneficial bacteria and an increase in pathogenic bacteria. Patescibacteria， Peptostreptococcus， Solobacterium， Filifactor， Moraxella， Porphyromonas endodontalis， and Capnocytophaga were identified as the key differential microbiota distinguishing

deficiency and blood stasis syndrome from non-

deficiency and blood stasis syndrome patients.

关键词

Keywords

references

刘明波，何新叶，杨晓红，等 . 《中国心血管健康与疾病报告2023》要点解读［J］. 中国心血管杂志， 2024 ， 29 （ 4 ）： 305 - 324 .

蓝宇，罗富锟，于悦，等 . 冠心病的中医认识与经方治疗策略［J］. 中国中药杂志， 2024 ， 49 （ 13 ）： 3684 - 3692 .

毕颖斐，王贤良，赵志强，等 . 冠心病现代中医证候特征的临床流行病学调查［J］. 中医杂志， 2017 ， 58 （ 23 ）： 2013 - 2019 .

黄国佑，李小成，熊杜 . 血管内超声评估不同中医证型冠心病动脉粥样硬化斑块特征［J］. 中国医学影像技术， 2024 ， 40 （ 1 ）： 51 - 54 .

付雪，庄杰钦，蔡海荣，等 . 冠心病气虚血瘀证与冠状动脉病变程度的相关性分析［J］. 中国中医急症， 2022 ， 31 （ 4 ）： 613 - 615，629 .

吴芸，周淑妮 . 冠心病不稳定型心绞痛患者中医证型分布及其与Gensini评分、炎症因子的关系［J］. 四川中医， 2021 ， 39 （ 10 ）： 63 - 66 .

LEVINE GN ， BATES ER ， BITTL JA ， et al . 2016 ACC/AHA guideline focused update on duration of dual antiplatelet therapy in patients with coronary artery disease： a report of the American College of Cardiology/American Heart Association Task Force on clinical practice guidelines ［J］. J Thorac Cardiovasc Surg ， 2016 ， 152 （ 5 ）： 1243 - 1275 .

中华中医药学会心血管分会 . 冠心病稳定型心绞痛中医诊疗指南［J］. 中医杂志， 2019 ， 60 （ 21 ）： 1880 - 1890 .

LOESCHE WJ . Microbiology and treatment of halitosis ［J］. Curr Infect Dis Rep ， 2003 ， 5 （ 3 ）： 220 - 226 .

TONELLI A ， LUMNGWENA EN ， NTUSI NAB . The oral microbiome in the pathophysiology of cardiovascular disease ［J］. Nat Rev Cardiol ， 2023 ， 20 （ 6 ）： 386 - 403 .

LIU M ， WANG X ， WU F ， et al . Variations of oral microbiome in chronic insomnia patients with different tongue features ［J］. Am J Chin Med ， 2020 ， 48 （ 4 ）： 923 - 944 .

LU C ， ZHU H ， ZHAO D ， et al . Oral-gut microbiome analysis in patients with metabolic-associated fatty liver disease having different tongue image feature ［J］. Front Cell Infect Microbiol ， 2022 ， 12 ： 787143 . doi： 10.3389/fcimb.2022.787143 http://dx.doi.org/10.3389/fcimb.2022.787143 .

LOZUPONE CA ， HAMADY M ， KELLEY ST ， et al . Quantitative and qualitative beta diversity measures lead to different insights into factors that structure microbial communities ［J］. Appl Environ Microbiol ， 2007 ， 73 （ 5 ）： 1576 - 1585 . doi： 10.3389/fcimb.2022.787143 http://dx.doi.org/10.3389/fcimb.2022.787143 .

BHUTE S ， PANDE P ， SHETTY SA ， et al . Molecular characterization and meta-analysis of gut microbial communities illustrate enrichment of prevotella and megasphaera in Indian subjects ［J］. Front Microbiol ， 2016 ， 7 ： 660 . doi： 10.3389/fmicb.2016.00660 http://dx.doi.org/10.3389/fmicb.2016.00660 .

ABUSLEME L ， DUPUY AK ， DUTZAN N ， et al . The subgingival microbiome in health and periodontitis and its relationship with community biomass and inflammation ［J］. ISME J ， 2013 ， 7 （ 5 ）： 1016 - 1025 .

LOURENÇO TG ， HELLER D ， SILVA-BOGHOSSIAN CM ， et al . Microbial signature profiles of periodontally healthy and diseased patients ［J］. J Clin Periodontol ， 2014 ， 41 （ 11 ）： 1027 - 1036 .

CAO X ， YUAN L . Gemella morbillorum infective endocarditis： a case report and literature review ［J］. Open Life Sci ， 2023 ， 18 （ 1 ）： 20220599 .

CHEN T ， YU WH ， IZARD J ， et al . The human oral microbiome database： a web accessible resource for investigating oral microbe taxonomic and genomic information ［J］. Database （Oxford）， 2010 ， 2010 ： baq013 . doi： 10. 1093/database/baq013 http://dx.doi.org/10.1093/database/baq013 .

LEONOV GE ， VARAEVA YR ， LIVANTSOVA EN ， et al . The complicated relationship of short-chain fatty acids and oral microbiome： a narrative review ［J］. Biomedicines ， 2023 ， 11 （ 10 ）： 2749 .

WELLS JM . Immunomodulatory mechanisms of lactobacilli ［J］. Microb Cell Fact ， 2011 ， 10 （ Suppl 1 ）： S17 .

OZUNA H ， SNIDER I ， BELIBASAKIS GN ， et al . Aggregatibacter actinomycetemcomitans and filifactor alocis： two exotoxin-producing oral pathogens ［J］. Front Oral Health ， 2022 ， 3 ： 981343 . doi： 10.3389/froh.2022. 981343 http://dx.doi.org/10.3389/froh.2022.981343 .

MONTEIRO MDE F ， CASATI MZ ， TAIETE T ， et al . Periodontal clinical and microbiological characteristics in healthy versus generalized aggressive periodontitis families ［J］. J Clin Periodontol ， 2015 ， 42 （ 10 ）： 914 - 921 .

CHHIBBER-GOEL J ， SINGHAL V ， BHOWMIK D ， et al . Linkages between oral commensal bacteria and atherosclerotic plaques in coronary artery disease patients ［J］. NPJ Biofilms Microbiomes ， 2016 ， 2 ： 7 . doi： 10. 1038/s41522-016-0009-7 http://dx.doi.org/10.1038/s41522-016-0009-7 .

查看原文

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Analysis of Clinical and Phenomics Characteristics of Patients with Phlegm-Stasis Binding Syndrome and Its Accompanied Patterns in Stable Angina Pectoris of Coronary Heart Disease

Effect of Xingeng No.Ⅱ Granules （心梗2号颗粒剂） on Left Ventricular Remodeling in Patients of ST-Segment Elevation Myocardial Infarction with Qi Deficiency and Blood Stasis Syndrome after PCI：An Open-Labbled Randomized-Controlled Trial

Pathogenesis Evolution and Staged Differentiation and Treatment Strategy of Atherosclerotic Plaques：from the Perspective of "Constraint-Putrefaction-Ulceration"

Effects of Yangxin Tongmai Formula （养心通脉方） on Methylation Key Genes and the PERK/ATF4/CHOP Signaling Pathway in Myocardial Tissue of Coronary Heart Disease Model Rats with Blood Stasis Syndrome

Construction of a Diagnostic Model for Coronary Heart Disease with Blood Stasis Syndrome Based on Objective Multi-Modal Data Fusion

Related Author

LI Chongchai

LI Han

LI Zheng

LI Zeng

ZHOU Yushi

AO Yuhan

XU Shuang

WANG Xue

Related Institution

Liaoning University of Traditional Chinese Medicine

Shenyang Health Service Center

Affiliated Hospital of Liaoning University of Traditional Chinese Medicine

Clinical Evaluation Center，China Academy of Chinese Medical Sciences

Dongfang Hospital，Beijing University of Chinese Medicine

Address：No.16, Nanxiao street, Dongzhimen, Dongcheng District, Beijing Postal code：100700
Tel：010-64035632 Email：jtcmcn@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010102007558号
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

Analysis of Tongue Coating Microbiota Characteristics in Coronary Heart Disease with Qi Deficiency and Blood Stasis Syndrome

DOI：10.13288/j.11-2166/r.2025.05.011

摘要

Abstract

关键词

Keywords

references