Effect of Acupuncture with the Method of Soothing the Liver and Regulating the Mind on the TLR4/NF-κB Pathway in the Hippocampus of Lipopolysaccharide-Induced Depression Model Mice

WU Bingxin; ZHOU Yi; HAN Sibo; HUANG Xichang; MA Junye; NING Baile; WANG Shanze; FU Wenbin

doi:10.13288/j.11-2166/r.2025.05.014

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Effect of Acupuncture with the Method of Soothing the Liver and Regulating the Mind on the TLR4/NF-κB Pathway in the Hippocampus of Lipopolysaccharide-Induced Depression Model Mice

更新时间：2025-03-06

- Effect of Acupuncture with the Method of Soothing the Liver and Regulating the Mind on the TLR4/NF-κB Pathway in the Hippocampus of Lipopolysaccharide-Induced Depression Model Mice
- Journal of Traditional Chinese Medicine Vol. 66, Issue 5, Pages: 526-531(2025)
- 作者机构：
  
  1.广州中医药大学第二临床医学院，广东省广州市白云区机场路12号，510006
  2.广州中医药大学第七临床医学院
  3.广州中医药大学第二附属医院
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2025.05.014
  CLC：
- Received：22 October 2024，
  
  Revised：2024-12-30，
  
  Published：02 March 2025
- 稿件说明：
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吴炳鑫,周易,韩思博等.“疏肝调神”针刺对脂多糖诱导抑郁模型小鼠海马TLR4/NF-κB通路的影响[J].中医杂志,2025,66(05):526-531.

WU Bingxin,ZHOU Yi,HAN Sibo,et al.Effect of Acupuncture with the Method of Soothing the Liver and Regulating the Mind on the TLR4/NF-κB Pathway in the Hippocampus of Lipopolysaccharide-Induced Depression Model Mice[J].Journal of Traditional Chinese Medicine,2025,66(05):526-531.
吴炳鑫,周易,韩思博等.“疏肝调神”针刺对脂多糖诱导抑郁模型小鼠海马TLR4/NF-κB通路的影响[J].中医杂志,2025,66(05):526-531. DOI： 10.13288/j.11-2166/r.2025.05.014.

WU Bingxin,ZHOU Yi,HAN Sibo,et al.Effect of Acupuncture with the Method of Soothing the Liver and Regulating the Mind on the TLR4/NF-κB Pathway in the Hippocampus of Lipopolysaccharide-Induced Depression Model Mice[J].Journal of Traditional Chinese Medicine,2025,66(05):526-531. DOI： 10.13288/j.11-2166/r.2025.05.014.

摘要

目的

探究“疏肝调神”针刺改善抑郁障碍的可能作用机制。

方法

将18只C57BL/6J小鼠随机分为空白组、模型组和针刺组，每组6只。针刺组和模型组通过腹腔注射脂多糖（LPS）诱导抑郁模型，空白组小鼠予腹腔注射等量生理盐水，每日1次，持续7天。造模的同时针刺组在造模第1天予“疏肝调神”针刺干预，每日1次，持续14天；空白组及模型组只捆绑不针刺。采用糖水偏好实验检测各组小鼠的糖水偏好率，采用旷场实验检测各组小鼠的中心停留时间以及活动总路程，采用强迫游泳实验检测各组小鼠的不动时间。采用HE染色观察各组小鼠海马形态变化。采用ELISA法检测各组小鼠海马组织中白细胞介素1β（IL-1β）、白细胞介素6（IL-6）和肿瘤坏死因子α（TNF-α）的含量。采用Western Blot法检测各组小鼠海马组织中TOLL样受体4（TLR4）、核因子κB（NF-κB）蛋白水平。

结果

与空白组比较，模型组糖水偏好率显著降低，中心停留时间显著减少，活动总路程显著缩短，强迫游泳不动时间，海马组织IL-1β、IL-6及TNF-α含量，TLR4及NF-κB蛋白水平显著增加（

＜0.01），海马神经细胞边界不清，排列疏松，少量神经细胞出现核皱缩深染；与模型组比较，针刺组糖水偏好率显著升高，中心停留时间显著增加，活动总路程显著延长，强迫游泳不动时间，海马组织IL-1β、IL-6及TNF-α含量，TLR4及NF-κB蛋白水平显著减少（

＜0.01），海马神经细胞较模型组排列紧密，核皱缩深染情况减少。

结论

“疏肝调神”针刺可显著改善抑郁样行为，其机制可能与抑制海马TLR4/NF-κB信号通路，减轻炎症反应有关。

Abstract

Objective

To explore the potential mechanism of acupuncture with the method of soothing the liver and regulating the mind in improving depressive disorder.

Methods

Eighteen C57BL/6J mice were randomly divided into blank group， model group， and acupuncture group， with 6 mice in each group. The model group and the acupuncture group were subjected to depression induction by intraperitoneal injection of lipopolysaccharide （LPS）， while the blank group received an equal volume of normal saline once daily for seven consecutive days. Concurrently， the acupuncture group received "soothing the liver and regulating the mind" acupuncture intervention starting from the first day of modeling， once daily for 14 days； whereas the blank group and the model group were only restrained without acupuncture. The sucrose preference test was used to assess sucrose preference rate， the open-field test to measure center stay time and total travel distance， and the forced swim test to evaluate immobility time. Hematoxylin-eosin （HE） staining was performed to observe hippocampal morphological changes. Enzyme-linked immunosorbent assay （ELISA） was used to detect levels of interleukin-1β （IL-1β）， interleukin-6 （IL-6）， and tumor necrosis factor-α （TNF-α） in hippocampal tissue. Western blot analysis was conducted to examine the protein expression levels of Toll-like receptor 4 （TLR4） and nuclear factor-κB （NF-κB） in the hippocampus.

Results

Compared to the blank group， the model group showed a significant reduction in sucrose preference rate， center stay time， and total travel distance， along with a significant increase in immobility time in the forced swim test， hippocampal IL-1β， IL-6， and TNF-α levels， as well as TLR4 and NF-κB protein expression （

＜0.01）， and the histological examination revealed blurred hippocampal neuronal boundaries， loose arrangement， and some neurons exhibiting nuclear pyknosis and deep staining. Compared to the model group， the acupuncture group demonstrated a significant increase in sucrose preference rate， center stay time， and total travel distance， along with a significant reduction in immobility time in the forced swim test， hippocampal IL-1β， IL-6， and TNF-α levels， and TLR4 and NF-κB protein expression （

＜0.01）， and the histological analysis showed that hippocampal neurons in the acupuncture group were more tightly arranged， with reduced nuclear pyknosis and deep staining.

Conclusion

Acupuncture with the "soothing the liver and regulating the mind" method can significantly improve depression-like behavior， potentially by inhibiting the hippocampal TLR4/NF-κB signaling pathway and alleviating inflammatory responses.

关键词

Keywords

references

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