特发性膜性肾病患者黄腻苔与白腻苔舌苔菌群特征的16S rRNA全长测序研究

doi:10.13288/j.11-2166/r.2025.06.009

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特发性膜性肾病患者黄腻苔与白腻苔舌苔菌群特征的16S rRNA全长测序研究

更新时间：2025-03-12

- 特发性膜性肾病患者黄腻苔与白腻苔舌苔菌群特征的16S rRNA全长测序研究
- Journal of Traditional Chinese Medicine Vol. 66, Issue 6, Pages: 596-603(2025)
- 作者机构：
  
  中国中医科学院西苑医院，北京市海淀区西苑操场路甲1号，100091
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2025.06.009
  CLC：
- Received：02 November 2024，
  
  Revised：2024-12-30，
  
  Published：17 March 2025
- 稿件说明：
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李艺含,王新慧,闫蕾等.特发性膜性肾病患者黄腻苔与白腻苔舌苔菌群特征的16S rRNA全长测序研究[J].中医杂志,2025,66(06):596-603.

[J]. Journal of Traditional Chinese Medicine, 2025, 66(6): 596-603.
李艺含,王新慧,闫蕾等.特发性膜性肾病患者黄腻苔与白腻苔舌苔菌群特征的16S rRNA全长测序研究[J].中医杂志,2025,66(06):596-603. DOI： 10.13288/j.11-2166/r.2025.06.009.

[J]. Journal of Traditional Chinese Medicine, 2025, 66(6): 596-603. DOI： 10.13288/j.11-2166/r.2025.06.009.

摘要

目的

探究特发性膜性肾病（IMN）患者黄腻苔和白腻苔的舌苔菌群特征。

方法

选择确诊为IMN的患者24例，其中黄腻苔13例组成黄腻苔组，白腻苔11例组成白腻苔组，另招募健康人10例组成健康组。收集各组舌苔菌群样本，运用16S rRNA全长测序技术对舌苔样本进行测序，对舌苔菌群Alpha多样性、Beta多样性、群落组成及差异进行微生物学信息分析，并基于MetaCyc数据库进行菌群功能预测分析。

结果

白腻苔组、黄腻苔组和健康组舌苔菌群之间Alpha多样性及Beta多样性差异无统计学意义（

＞0.05）。与健康组比较，白腻苔组和黄腻苔组卟啉单胞菌属的相对丰度减少（

＜0.05）。黄腻苔组弯曲杆菌属、弯曲杆菌_sp002139875和胎儿弯曲杆菌的相对丰度较白腻苔和健康组增高；白腻苔组生痰二氧化碳噬纤维菌的相对丰度较其他两组增高（

＜0.05）。菌群功能预测结果显示，与健康组比较，白腻苔组和黄腻苔组的磺酸内酯降解超级途径、雄烯二酮降解Ⅰ（需氧）、甲苯降解通路途径下调（

＜0.05）；与白腻苔组比较，黄腻苔组丙酮酸发酵生成丙酸Ⅰ、琥珀酸发酵生成丁酸盐、甲苯降解途径下调，而D-葡萄糖醛酸和D-半乳糖醛酸降解超级途径上调（

＜0.05）。

结论

IMN患者舌苔菌群失调，其中卟啉单胞菌属可能成为IMN的微生物学表征；弯曲杆菌属、弯曲杆菌_sp002139875、胎儿弯曲杆菌可能是IMN黄腻苔的标志菌；生痰二氧化碳噬纤维菌可能是IMN白腻苔的标志菌种。

Abstract

Objective

To explore the characteristics of tongue coating microbiomes of yellow and white greasy coatings in patients with idiopathic membranous nephropathy （IMN）.

Methods

Twenty-four patients diagnosed with IMN were selected. Thirteen patients with yellow greasy coating were assigned to the yellow greasy group， and eleven patients with white greasy coating were assigned to the white greasy group. Additionally， ten healthy individuals were recruited as a control group. Tongue coating microbiome samples were collected from each group and analyzed using 16S rRNA full-length sequencing. The microbial diversity （Alpha and Beta diversity）， community composition， and differences were analyzed. Furthermore， functional prediction of the microbiomes was performed based on the MetaCyc database.

Results

There was no significant difference in Alpha and Beta diversity among the tongue coating microbiomes of the yellow greasy group， white greasy group， and healthy group （

＞0.05）. Compared to the healthy group， the relative abundance of

Porphyromonas

species was reduced in both the white greasy and yellow greasy groups （

＜0.05）. The yellow greasy group showed an increased relative abundance of

Campylobacter

species，

Campylobacter_sp002139875

， and

Fetal Campylobacter

， compared to the white greasy and healthy groups. The white greasy group had a higher relative abundance of

Fibrobacteres

species， which are involved in phlegm production， than the other two groups （

＜0.05）. Functional prediction analysis revealed that， compared to the healthy group， the white greasy and yellow greasy grou

ps showed downregulation in the pathways of sulfonate ester degradation， androstenedione degradation Ⅰ （aerobic）， and toluene degradation （

＜0.05）. In comparison to the white greasy group， the yellow greasy group showed downregulation in the pathways of pyruvate fermentation to propionate Ⅰ， succinate fermentation to butyrate， and toluene degradation， while upregulating the superpathway of D-glucuronic acid and D-galacturonic acid degradation （

＜0.05）.

Conclusion

The tongue coating microbiome in IMN patients is dysregulated， and

Porphyromonas

species may serve as a microbiological marker for IMN.

Campylobacter

，

Campylobacter_sp002139875

， and

Fetal Campylobacter

could be signature bacteria for yellow greasy tongue coating in IMN， while

Fibrobacteres

species associated with phlegm production may be signature bacteria for white greasy tongue coating.

关键词

Keywords

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16S rRNA Full-length Sequencing Study of Tongue Coating Microbiome Characteristics of Yellow and White Greasy Coatings in Patients with Idiopathic Membranous Nephropathy

LI Yihan ， WANG Xinhui ， YAN Lei ， YU Renhuan

Xiyuan Hospital ， China Academy of Chinese Medical Sciences ， Beijing ， 100091

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