Effect of <italic style="font-style: italic">Modified Gegen Qinlian Decoction</italic> （加味葛根芩连汤） on the Intestinal Mucus Barrier and Intestinal Stem Cell Proliferation and Differentiation in Ulcerative Colitis Model Mice

HUANG Jinke; ZHANG Jiaqi; WANG Fengyun; TANG Xudong

doi:10.13288/j.11-2166/r.2025.09.012

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Effect of Modified Gegen Qinlian Decoction （加味葛根芩连汤） on the Intestinal Mucus Barrier and Intestinal Stem Cell Proliferation and Differentiation in Ulcerative Colitis Model Mice

更新时间：2025-04-30

- Effect of Modified Gegen Qinlian Decoction （加味葛根芩连汤） on the Intestinal Mucus Barrier and Intestinal Stem Cell Proliferation and Differentiation in Ulcerative Colitis Model Mice
- Journal of Traditional Chinese Medicine Vol. 66, Issue 9, Pages: 941-947(2025)
- 作者机构：
  
  中国中医科学院西苑医院脾胃病研究所，北京市海淀区西苑操场1号，100091
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2025.09.012
  CLC：
- Received：08 April 2024，
  
  Revised：2024-10-30，
  
  Published：02 May 2025
- 稿件说明：
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黄金科,张佳琪,王凤云等.加味葛根芩连汤对溃疡性结肠炎模型小鼠肠黏液屏障和肠道干细胞增殖分化的影响[J].中医杂志,2025,66(09):941-947.

HUANG Jinke,ZHANG Jiaqi,WANG Fengyun,et al.Effect of Modified Gegen Qinlian Decoction （加味葛根芩连汤） on the Intestinal Mucus Barrier and Intestinal Stem Cell Proliferation and Differentiation in Ulcerative Colitis Model Mice[J].Journal of Traditional Chinese Medicine,2025,66(09):941-947.
黄金科,张佳琪,王凤云等.加味葛根芩连汤对溃疡性结肠炎模型小鼠肠黏液屏障和肠道干细胞增殖分化的影响[J].中医杂志,2025,66(09):941-947. DOI： 10.13288/j.11-2166/r.2025.09.012.

HUANG Jinke,ZHANG Jiaqi,WANG Fengyun,et al.Effect of Modified Gegen Qinlian Decoction （加味葛根芩连汤） on the Intestinal Mucus Barrier and Intestinal Stem Cell Proliferation and Differentiation in Ulcerative Colitis Model Mice[J].Journal of Traditional Chinese Medicine,2025,66(09):941-947. DOI： 10.13288/j.11-2166/r.2025.09.012.

摘要

目的

基于肠黏液屏障探讨加味葛根芩连汤治疗溃疡性结肠炎的可能作用机制。

方法

将30只C57BL/6小鼠随机分为对照组、模型组及加味葛根芩连汤组各10只。除对照组外，其余各组小鼠采用葡聚糖硫酸钠盐构建溃疡性结肠炎小鼠模型。造模同时加味葛根芩连汤组小鼠按体质量给予20g/kg的加味葛根芩连汤水煎剂灌胃，对照组和模型组按小鼠体重给予0.2 ml/20 g纯水灌胃，每日1次，连续7天。最后一次灌胃次日，比较各组小鼠体质量、疾病活动指数（DAI）评分、脾重、结肠长度；HE染色观察结肠组织的病理学改变；免疫荧光检测结肠组织黏蛋白2（MUC2）及富含亮氨酸重复G蛋白偶联受体5（Lgr5）表达水平；AB/PAS染色检测结肠组织的杯状细胞数量；免疫组化检测肠黏膜组织基尔67抗原（Ki67）水平。

结果

HE染色结果显示，对照组小鼠结肠黏膜组织结构完整；模型组小鼠结肠黏膜上皮结构遭受破坏明显，黏膜固有层内可见大量炎性细胞浸润。加味葛根芩连汤组小鼠的黏膜组织结构部分缺失，可见少量炎性细胞浸润。与对照组比较，模型组小鼠体质量、结肠长度降低，DAI评分及脾重升高，MUC2、Ki67、Lgr5蛋白水平及杯状细胞数量均降低（

＜0.01）；与模型组比较，加味葛根芩连汤组小鼠体质量、结肠长度升高，DAI评分及脾重降低，MUC2、Ki67、Lgr5蛋白水平及杯状细胞数量均升高（

＜0.05或

＜0.01）。

结论

加味葛根芩连汤对溃疡性结肠炎模型小鼠相关症状及病理组织损伤具有改善作用，其机制可能与恢复肠道干细胞增殖并分化为杯状细胞，进而促进肠黏液屏障修复相关。

Abstract

Objective

To explore the possible mechanism of

Modified Gegen Qinlian Decoction

（加味葛根芩连汤， MGQD） in the treatment of ulcerative colitis （UC） based on intestinal mucus barrier.

Methods

Thirty C57BL/6 mice were randomly divided into a control group， a model group and a MGQD group with 10 mice in each. Dextran Sulfate Sodium Salt （DSS） was used to construct the UC model in all groups except for the control group. Meanwhile， mice in the MGQD group were given 20 g/kg of MGQD decoction by gavage according to their body weight， while those in the control group and model group were given 0.2 ml/20 g of pure water by gavage， once a day for 7 consecutive days. On the day following the last gavage， the body weight， disease activity index （DAI） score， spleen weight， and colon length were compared. The pathological changes of the intestinal mucosal tissues were observed by HE staining； the protein expression levels of mucin 2 （MUC2） and leucine-rich repeat G protein-coupled receptor 5 （Lgr5） in the intestinal mucosal tissues were detected by immunofluorescence； the cuprocytes in the intestinal mucosal tissues were detected by AB/PAS staining； and the expression level of Ki67 in the intestinal mucosal tissues was detected by immunohistochemistry.

Results

HE staining showed that the colon mucosal tissue of the mice in the control group was intact. In the model group， the colon mucosal epithelial structure was severely damaged， with a large amount of inflammatory cell infiltration in the mucosal propria. In the MGQD group， the mucosal tissue structure was partially lost， with a small amount of inflammatory cell infiltration.The body weight and colon length of mice in the model group decreased significantly compared to those in the control group， while DAI scores and spleen weight increased， and the levels of MUC2， Ki67， Lgr5 proteins， and the number of goblet cells were significantly reduced （

＜0.01）. Compared to the model group， the MGQD group had increased body weight of mice， colon length， and decreased DAI scores and spleen weight； the levels of MUC2， Ki67， Lgr5 proteins， and the number of goblet cells were increased （

＜0.05 or

＜0.01）.

Conclusion

MGQD has a favorable ameliorative effect on UC-related symptoms and pathological tissue damage， and its mechanism of action may be related to the restoration of the prolife-ration and differentiation of intestinal stem cells into goblet cells， thereby promoting the repair of the intestinal mucus barrier.

关键词

Keywords

references

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Dongfang Hospital，Beijing University of Chinese Medicine

School of Traditional Chinese Medicine，Beijing University of Chinese Medicine

Institute of Spleen and Stomach Diseases，Xiyuan Hospital，China Academy of Chinese Medical Sciences

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Jiangsu Provincial Hospital of Chinese Medicine/Affiliated Hospital of Nanjing University of Chinese Medicine

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Effect of Modified Gegen Qinlian Decoction （加味葛根芩连汤） on the Intestinal Mucus Barrier and Intestinal Stem Cell Proliferation and Differentiation in Ulcerative Colitis Model Mice

DOI：10.13288/j.11-2166/r.2025.09.012

摘要

Abstract

关键词

Keywords

references