Effect of <italic style="font-style: italic">Erchen Decoction </italic>（二陈汤） on Serum Leptin and Expression of LepR，POMC，and NPY in Hypothalamus of Metabolic Syndrome Model Mice with Phlegm Syndrome

YANG Menghan; LI Yuanyuan; ZHENG Xiujuan; XIONG Wenhui; HUANG Xirui; GAO Bizhen

doi:10.13288/j.11-2166/r.2025.09.013

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Effect of Erchen Decoction （二陈汤） on Serum Leptin and Expression of LepR，POMC，and NPY in Hypothalamus of Metabolic Syndrome Model Mice with Phlegm Syndrome

更新时间：2025-04-30

- Effect of Erchen Decoction （二陈汤） on Serum Leptin and Expression of LepR，POMC，and NPY in Hypothalamus of Metabolic Syndrome Model Mice with Phlegm Syndrome
- Journal of Traditional Chinese Medicine Vol. 66, Issue 9, Pages: 948-954(2025)
- 作者机构：
  
  1.福建中医药大学中医学院，福建省福州市闽侯上街邱阳路1号，350122
  2.福建中医药大学中西医结合学院
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2025.09.013
  CLC：
- Received：04 December 2024，
  
  Revised：2025-02-24，
  
  Published：02 May 2025
- 稿件说明：
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杨梦晗,李缘缘,郑秀娟等.二陈汤对代谢综合征痰证模型小鼠血清瘦素及下丘脑LepR、POMC、NPY表达的影响[J].中医杂志,2025,66(09):948-954.

YANG Menghan,LI Yuanyuan,ZHENG Xiujuan,et al.Effect of Erchen Decoction （二陈汤） on Serum Leptin and Expression of LepR，POMC，and NPY in Hypothalamus of Metabolic Syndrome Model Mice with Phlegm Syndrome[J].Journal of Traditional Chinese Medicine,2025,66(09):948-954.
杨梦晗,李缘缘,郑秀娟等.二陈汤对代谢综合征痰证模型小鼠血清瘦素及下丘脑LepR、POMC、NPY表达的影响[J].中医杂志,2025,66(09):948-954. DOI： 10.13288/j.11-2166/r.2025.09.013.

YANG Menghan,LI Yuanyuan,ZHENG Xiujuan,et al.Effect of Erchen Decoction （二陈汤） on Serum Leptin and Expression of LepR，POMC，and NPY in Hypothalamus of Metabolic Syndrome Model Mice with Phlegm Syndrome[J].Journal of Traditional Chinese Medicine,2025,66(09):948-954. DOI： 10.13288/j.11-2166/r.2025.09.013.

摘要

目的

探讨二陈汤改善代谢综合征（MS）痰证的可能作用机制。

方法

将40只小鼠随机分成空白组10只和造模组30只。造模组小鼠采用高脂饮食诱导建立MS痰证小鼠模型。将30只造模成功的小鼠随机分为模型组、二陈汤组和二甲双胍组，每组10只。二陈汤组小鼠给予二陈汤11.1 g/（kg·d）灌胃，二甲双胍组给予二甲双胍溶液0.4 g/（kg·d）灌胃，空白组和模型组则给予灭菌饮用水0.02 ml/（g·d）灌胃，每日1次，共干预4周。记录各组小鼠体质量、腹围、体长、Lee's指数、进食量，检测空腹血糖和血脂［包括甘油三酯（TG）、总胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）］水平，ELISA法检测血清瘦素水平，HE染色观察肝脏组织病理变化，Western Blot和q-PCR法分别检测下丘脑组织中瘦素受体（LepR）、阿黑皮素原（POMC）、神经肽Y（NPY）蛋白水平和mRNA表达，免疫荧光法检测下丘脑弓状核区域POMC和NPY荧光表达量。

结果

病理结果显示，模型组小鼠肝细胞见大量脂肪空泡，肝脏脂肪沉积明显增多；二陈汤组和二甲双胍组脂肪空泡减少，肝脏脂肪沉积程度减轻。与空白组比较，模型组小鼠肝脏脂肪沉积，小鼠体质量、腹围、Lee's指数升高，进食量增加，空腹血糖、TG、TC、LDL-C、血清瘦素水平升高，HDL-C水平降低，LepR、POMC蛋白水平及其mRNA表达降低，NPY蛋白水平及其mRNA表达升高，下丘脑弓状核区域POMC荧光表达量降低、NPY荧光表达量升高（

＜0.05或

＜0.01）；与模型组比较，二陈汤组和二甲双胍组小鼠上述各指标均显著改善（

＜0.05或

＜0.

01）；与二陈汤组比较，二甲双胍组体质量、NPY荧光表达量降低，HDL-C水平升高（

＜0.05或

＜0.01）。

结论

二陈汤可以下调血清瘦素水平，改善代谢综合征痰证糖脂代谢，其作用机制可能是减轻肝脏脂肪沉积，影响下丘脑中与摄食活动相关的神经肽的表达。

Abstract

Objective

To explore the potential mechanism of

Erchen Decoction

（二陈汤， ECD） in improving metabolic syndrome （MS） with phlegm syndrome.

Methods

Forty mice were randomly divided into a blank group of 10 mice and a modeling group of 30 mice. The MS model with phlegm syndrome was induced in the modeling group by high-fat diet. Thirty successfully modeled mice were randomly divided into a model group， a ECD group， and a metformin group， with 10 mice in each group. The ECD group was given 0.4 g/（kg·d） of ECD， while the metformin group was intervened with 11.1 g/（kg·d） of metformin solution， and the blank group and the model group were given 0.02 ml/（g·d） of sterilized drinking water， all by gavage， once daily for 4 weeks. Body weight， abdominal circumfe-rence， body length， Lee's index and food intake were recorded. Blood glucose and blood lipid levels including fasting blood glucose， triglycerides （TG）， total cholesterol （TC）， high-density lipoprotein cholesterol （HDL-C）， and low-density lipoprotein cholesterol （LDL-C） were measured. ELISA was used to detect serum leptin levels， while HE staining was used to observe liver pathological changes. Western Blot and q-PCR were used to detect protein and mRNA expression of hypothalamic leptin receptor （LepR）， pro melanocortin （POMC）， and neuropeptide Y （NPY） in the hypothalamus. Immunofluorescence was used to detect fluorescence expression of POMC and NPY in the hypothalamic arcuate nucleus region.

Results

Pathological results showed that the mice in the model group had numerous fat vacuoles in hepatocytes and significant liver fat deposition， while the ECD and metformin groups showed reduced fat vacuoles and le

ss liver fat deposition. Compared to those in the blank group， the mice in the model group mice showed liver fat deposition， increased body weight， abdominal circumference， Lee's index and food intake； fasting blood glucose， TG， TC， LDL-C， and serum leptin levels were elevated， while HDL-C was decreased； the expression of LepR， POMC protein levels and their mRNA expression decreased， while the protein level and mRNA expression of NPY increased； the fluorescence expression of POMC in the arcuate nucleus was reduced， while NPY fluorescence expression increased （

＜0.05 or

＜0.01）. Compared to the model group， the ECD group and metformin group showed significant improvements in the above indicators （

＜0.05 or

＜0.01）. Compared to the ECD group， the metformin group showed a reduction in body weight and NPY fluorescence expression， and an increase in HDL-C levels （

＜0.05 or

＜0.01）.

Conclusion

ECD can downregulate serum leptin levels and improve glucose and lipid metabolism in the MS of phlegm syndrome. Its mechanism of action may be to reduce liver fat deposition and thereafter affect the expression of neuropeptides related to feeding activity in the hypothalamus.

关键词

Keywords

references

ACHILA OO ， ARAYA M ， BERHE AB ， et al . Metabolic syndrome， associated factors and optimal waist circumfe-rence cut points： findings from a cross-sectional community-based study in the elderly population in Asmara， Eritrea ［J］. BMJ Open ， 2022 ， 12 （ 2 ）： e52296 .

王春花，王敏，刘鸿丽，等 . 瘦素与代谢综合征相关性的研究进展［J］. 当代医药论丛， 2019 ， 17 （ 13 ）： 35 - 37 .

MARTÍNEZ-UÑA M ， LÓPEZ-MANCHEÑO Y ， DIÉGUEZ C ， et al . Unraveling the role of leptin in liver function and its relationship with liver diseases ［J］. Int J Mol Sci ， 2020 ， 21 （ 24 ）： 9368 .

BUETTNER C ， POCAI A ， MUSE ED ， et al . Critical role of STAT3 in leptin's metabolic actions ［J］. Cell Metab ， 2006 ， 4 （ 1 ）： 49 - 60 .

陈淑娇，李灿东，赖新梅，等 . 160例代谢综合征患者中医证候特点研究［J］. 中华中医药杂志， 2015 ， 30 （ 3 ）： 689 - 692 .

中国医师协会中西医结合医师分会内分泌与代谢病学专业委员会 . 代谢综合征病证结合诊疗指南［J］. 世界中医药， 2023 ， 18 （ 22 ）： 3157 - 3166 .

侯明玉，田财军 . 从痰演变过程论述二陈汤［J］. 光明中医， 2024 ， 39 （ 22 ）： 4486 - 4489 .

严晓丹，唐莉玲，钱长晖，等 . 二陈汤抑制高脂饲养肥胖小鼠炎症和肝脏脂肪变性作用研究［J］. 福建中医药， 2023 ， 54 （ 10 ）： 11 - 14，19 .

魏佳，曾华霖，林书颖，等 . 代谢综合征痰证大鼠模型建立与评价的实验研究［J］. 辽宁中医杂志， 2022 ， 49 （ 6 ）： 195 - 199 .

魏佳，曾华霖，林书颖，等 . 代谢综合征痰证动物模型评价标准研究［J］. 中国中医基础医学杂志， 2022 ， 28 （ 10 ）： 1606 - 1609，1646 .

GAO BZ ， CHEN JC ， LIAO LH ， et al . Erchen decoction prevents high-fat diet induced metabolic disorders in C57BL/6 mice ［J］. Evid Based Complement Alternat Med ， 2015 ： 501272 . doi： 10.1155/2015/501272 http://dx.doi.org/10.1155/2015/501272 .

吕文琦，陈艳，谢云志 . 中西医结合治疗痰湿蕴脾型老年代谢综合征42例临床观察［J］. 中国民族民间医药， 2024 ， 33 （ 21 ）： 92 - 97 .

张光辉 . 在代谢综合征疾病临床治疗中采用二甲双胍对血糖、血脂水平的影响［J］. 黑龙江中医药， 2021 ， 50 （ 3 ）： 87 - 88 .

PASANISI P ， OLIVERIO A ， BALDASSARI I ， et al . Metformin treatment with or without mediterranean diet for the prevention of age-related diseases in people with metabolic syndrome： the mememe randomized trial ［J］. Diabetes Care ， 2025 ， 48 （ 2 ）： 265 - 272 .

周慧敏 . 温肾健脾化痰方调控单纯性肥胖大鼠脂代谢及瘦素抵抗的机制研究［D］. 武汉：湖北中医药大学， 2017 .

OBRADOVIC M ， SUDAR-MILOVANOVIC E ， SOSKIC S ， et al . Leptin and obesity： role and clinical implication ［J］. Front Endocrinol （Lausanne）， 2021 ， 12 ： 585887 . doi： 10.3389/fendo.2021.585887 http://dx.doi.org/10.3389/fendo.2021.585887 .

CAVADAS C ， AVELEIRA CA ， SOUZA GF ， et al . The pathophysiology of defective proteostasis in the hypothalamus-from obesity to ageing ［J］. Nat Rev Endocrinol ， 2016 ， 12 （ 12 ）： 723 - 733 .

LIU H ， DU T ， LI C ， et al . STAT3 phosphorylation in central leptin resistance ［J］. Nutr Metab （Lond）， 2021 ， 18 （ 1 ）： 39 .

ABERDEIN N ， DAMBRINO RJ ， CARMO JMDO ， et al . Role of PTP1B in POMC neurons during chronic high-fat diet： sex differences in regulation of liver lipids and glucose tolerance ［J］. Am J Physiol Regul Integr Comp Physiol ， 2018 ， 314 （ 3 ）： R478 - R488 .

杨威 . 利拉鲁肽影响肥胖大鼠下丘脑促食、抑食神经元凋亡的机制研究［D］. 南宁：广西医科大学， 2020 .

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Effect of Erchen Decoction （二陈汤） on Serum Leptin and Expression of LepR，POMC，and NPY in Hypothalamus of Metabolic Syndrome Model Mice with Phlegm Syndrome

DOI：10.13288/j.11-2166/r.2025.09.013

摘要

Abstract

关键词

Keywords

references