Effects of Zhimu （<italic style="font-style: italic">Anemarrhena asphodeloides</italic>）-Huangbai （<italic style="font-style: italic">Phellodendron amurense</italic>） Medicinal Pair on Femoral Microstructure and Osteogenic-Adipogenic Differentiation in Ovariectomized Osteoporosis Model Rats

LI Chuncai; YUAN Mingxing; LI Jiawei; DENG Jing; SHEN Chongyang; LIU Yuan

doi:10.13288/j.11-2166/r.2025.16.013

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Effects of Zhimu （Anemarrhena asphodeloides）-Huangbai （Phellodendron amurense） Medicinal Pair on Femoral Microstructure and Osteogenic-Adipogenic Differentiation in Ovariectomized Osteoporosis Model Rats

更新时间：2025-08-13

- Effects of Zhimu （Anemarrhena asphodeloides）-Huangbai （Phellodendron amurense） Medicinal Pair on Femoral Microstructure and Osteogenic-Adipogenic Differentiation in Ovariectomized Osteoporosis Model Rats
- Journal of Traditional Chinese Medicine Vol. 66, Issue 16, Pages: 1704-1710(2025)
- 作者机构：
  
  成都中医药大学，四川省成都市温江区柳台大道1166号，611137
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2025.16.013
  CLC：
- Received：28 March 2025，
  
  Revised：2025-05-27，
  
  Published：17 August 2025
- 稿件说明：
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李春财,原明星,李嘉炜等.知母-黄柏药对对卵巢切除骨质疏松症模型大鼠股骨微结构及骨脂分化的影响[J].中医杂志,2025,66(16):1704-1710.

LI Chuncai,YUAN Mingxing,LI Jiawei,et al.Effects of Zhimu （Anemarrhena asphodeloides）-Huangbai （Phellodendron amurense） Medicinal Pair on Femoral Microstructure and Osteogenic-Adipogenic Differentiation in Ovariectomized Osteoporosis Model Rats[J].Journal of Traditional Chinese Medicine,2025,66(16):1704-1710.
李春财,原明星,李嘉炜等.知母-黄柏药对对卵巢切除骨质疏松症模型大鼠股骨微结构及骨脂分化的影响[J].中医杂志,2025,66(16):1704-1710. DOI： 10.13288/j.11-2166/r.2025.16.013.

LI Chuncai,YUAN Mingxing,LI Jiawei,et al.Effects of Zhimu （Anemarrhena asphodeloides）-Huangbai （Phellodendron amurense） Medicinal Pair on Femoral Microstructure and Osteogenic-Adipogenic Differentiation in Ovariectomized Osteoporosis Model Rats[J].Journal of Traditional Chinese Medicine,2025,66(16):1704-1710. DOI： 10.13288/j.11-2166/r.2025.16.013.

摘要

目的

探究知母-黄柏药对缓解绝经后骨质疏松症（PMOP）的可能作用机制。

方法

将60只未孕雌性SD大鼠随机分成空白组、模型组、知母-黄柏低剂量组、知母-黄柏高剂量组和雌二醇组，每组12只。除空白组外，其余各组大鼠切除双侧卵巢构建PMOP大鼠模型，空白组大鼠仅切除卵巢周围少量脂肪组织。大鼠卵巢切除1周后，知母-黄柏低、高剂量组分别给予知母和黄柏浓缩液1.8、7.2 g/（kg·d）灌胃，雌二醇组给予雌二醇溶液0.09 mg/（kg·d）灌胃，空白组和模型组给予10 ml/（kg·d）生理盐水灌胃，均每天1次，持续12周。取材前记录大鼠体质量，取大鼠完整子宫并计算子宫指数；ELISA法检测血清中白细胞介素1β（IL-1β）、白细胞介素6（IL-6）和肿瘤坏死因子α（TNF-α）水平；Micro CT检测股骨微结构相关参数［包括骨体积/组织体积（BV/TV）、骨小梁数量（Tb.N）、骨小梁厚度（Tb.Th）、骨密度（BMD）、骨小梁分离度（Tb.Sp）和皮质骨面积（Ct.Ar）］；HE染色法观察股骨病理学变化；RT-qPCR法检测股骨组织中骨脂分化相关因子［包括Runt相关转录因子2（

Runx2

）、骨形态发生蛋白2（

BMP

）、过氧化物酶体增殖物激活受体γ（

PPARγ

）、趋化素（

Chemerin

）、趋化因子样受体1（

CMKLR1

）］mRNA表达。

结果

与空白组比较，模型组大鼠体质量升高，子宫指数、BV/TV、Tb.N、Tb.Th、BMD降低，Tb.Sp和血清中IL-1β、IL-6、TNF-α水平升高，股骨组织中

Runx2、BMP

mRNA表达降低，

PPARγ、Chemerin

和

CMKLR1

mRNA表达升高（

＜0.01）；HE染色显示，股骨组织骨小梁数量减少、排列稀疏，髓腔明显增大，含有大量脂肪细胞。与模型组比较，知母-黄柏低、高剂量组和雌二醇组上述各指标均有不同程度改善（

＜0.05或

＜0.01）；HE染色显示，骨小梁数量明显增多，排列较整齐，脂肪细胞明显减少。与知母-黄柏低剂量组比较，知母-黄柏高剂量组子宫指数、BMD升高，体质量及

PPARγ、Chemerin

mRNA表达降低（

＜0.05或

＜0.01）。与知母-黄柏高剂量组比较，雌二醇组子宫指数、BV/TV、Tb.N、Tb.Th、BMD及

BMP

mRNA表达降低，IL-1β、TNF-α、IL-6水平，Tb.Sp及

PPARγ、Chemerin、CMKLR1

mRNA表达升高（

＜0.05或

＜0.01）。

结论

知母-黄柏药对可以缓解PMOP骨质流失，其作用机制与降低炎症因子水平，纠正骨髓间充质干细胞骨脂分化紊乱，促进骨髓间充质干细胞成骨细胞分化有关。

Abstract

Objective

To investigate the potential mechanisms of Zhimu （

Anemarrhena asphodeloides

）-Huangbai （

Phellodendron amurense

） medicinal pair in alleviating postmenopausal osteoporosis （PMOP）.

Methods

Sixty unpregnant female SD rats were randomly divided into five groups， blank group， model group， low-dose Zhimu-Huangbai group， high-dose Zhimu-Huangbai group， and estradiol group， with 12 rats in each group. Except for the blank group， all other groups had their ovaries removed to create PMOP rat models， while the blank group only had the fat tissue around the ovaries removed. One week after the ovarian removal， the low-dose and high-dose Zhimu-Huangbai groups received concentrated solution of Zhimu and Huangbai with 1.8， 7.2 g/（kg·d） via gavage， the estradiol group received estradiol solution 0.09 mg/（kg·d） via gavage， and the blank group and the model group received 10 ml/（kg·d） of normal saline via gavage， once daily for 12 weeks. Before sampling， the body mass of the rats was recorded， and uterine tissue was taken to calculate the uterine index. The levels of interleukin-1β （IL-1β）， interleukin-6 （IL-6） and tumor necrosis factor-α （TNF-α） in serum were detected by ELISA； micro CT was used to examine the parameters of femoral microstructure， including bone volume/tissue volume （BV/TV）， trabecular number （Tb.N）， trabecular thickness （Tb.Th）， bone mineral density （BMD）， trabecular separation （Tb.Sp）， and cortical bone area （Ct.Ar）. HE staining was used to observe pathological changes in the femur

； RT-qPCR was used to detect the mRNA expression of osteogenic-adipogenic differentiation-related factors in femoral tissue， including Runt-related transcription factor 2 （

Runx2

）， bone morphogenetic protein 2 （

BMP

）， peroxisome proliferator-activated receptor γ （

PPARγ

），

chemerin

and chemokine-like receptor 1 （

CMKLR1

）.

Results

Compared with the blank group， the model group showed a significant increase in body mass， a significant decrease in the uterine index， BV/TV， Tb.N， Tb.Th and BMD， a significant increase in Tb.Sp， and serum IL-1β， IL-6， and TNF-α levels， a significant reduction of mRNA expression of

Runx2

and

BMP

in bone tissue， and a significant increased mRNA expression of

PPARγ

，

chemerin

， and

CMKLR1

（

＜0.01）. HE staining revealed that the femoral tissue showed a reduction and sparsity of trabeculae， a significant enlargement of the medullary cavity， and a large number of fat cells. Compared to the model group， the low-dose， high-dose Zhimu-Huangbai groups， and estradiol group showed significant improvements in all the above-mentioned indicators （

＜0.05 or

＜0.01）. HE staining revealed a significant increase in trabeculae， more organized arrangement， and a marked reduction in fat cells. Compared to low-dose Zhimu-Huangbai group， the high-dose Zhimu-Huangbai group exhibited a significant increase in the uterine index and BMD， and a significant reduction in body mass and

PPARγ

and

Chemerin

mRNA expression （

＜0.05 or

＜0.01）. Compared to high-dose Zhimu-Huangbai group， the estradiol group showed a decrease in uterine index， BV/TV， Tb.N， Tb.Th， BMD， and

BMP

mRNA expression， while the levels of IL-1β， TNF-α， and IL-6， as well

as Tb.Sp and the mRNA expressions of

PPARγ

，

chemerin

， and

CMKLR1

increased （

＜0.05 or

＜0.01）.

Conclusion

The Zhimu-Huangbai medicinal pair can alleviate PMOP bone loss， and its mechanism of action is related to reducing the levels of inflammatory factors， correcting the disorder of osteogenic-adipogenic differentiation of bone marrow mesenchymal stem cells （BMSCs）， and promoting the differentiation of BMSCs into osteoblasts.

关键词

Keywords

references

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Effects of Zhimu （Anemarrhena asphodeloides）-Huangbai （Phellodendron amurense） Medicinal Pair on Femoral Microstructure and Osteogenic-Adipogenic Differentiation in Ovariectomized Osteoporosis Model Rats

DOI：10.13288/j.11-2166/r.2025.16.013

摘要

Abstract

关键词

Keywords

references