Effects of Different Degrees of "Dryness Pathogen" on the PGD<sub>2</sub>/EOS Axis in the Lung Tissue of Bronchial Asthma Model Rats

JIA Junjie; JIANG De; CHEN Yifei; XIONG Qianran; XIN Zhiyuan; YU Ruonan

doi:10.13288/j.11-2166/r.2026.20.012

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Effects of Different Degrees of "Dryness Pathogen" on the PGD₂/EOS Axis in the Lung Tissue of Bronchial Asthma Model Rats

更新时间：2026-10-10

- Effects of Different Degrees of "Dryness Pathogen" on the PGD₂/EOS Axis in the Lung Tissue of Bronchial Asthma Model Rats
- Journal of Traditional Chinese Medicine Vol. 67, Issue 20, Pages: 2219-2226(2026)
- 作者机构：
  
  新疆医科大学，新疆维吾尔自治区乌鲁木齐市水磨沟区尚德北路567号，830017
- 作者简介：
- 基金信息：
- DOI：10.13288/j.11-2166/r.2026.20.012
  CLC：
- Received：25 February 2026，
  
  Revised：2026-06-24，
  
  Accepted：12 June 2026，
  
  Published：17 October 2026
- 稿件说明：
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贾珺捷,姜德,陈乙菲等.不同程度“燥邪”对支气管哮喘模型大鼠肺组织PGD₂/EOS轴的影响[J].中医杂志,2026,67(20):2219-2226. DOI: 10.13288/j.11-2166/r.2026.20.012.

JIA Junjie,JIANG De,CHEN Yifei,et al.Effects of Different Degrees of "Dryness Pathogen" on the PGD₂/EOS Axis in the Lung Tissue of Bronchial Asthma Model Rats[J].Journal of Traditional Chinese Medicine,2026,67(20):2219-2226. DOI: 10.13288/j.11-2166/r.2026.20.012.
贾珺捷,姜德,陈乙菲等.不同程度“燥邪”对支气管哮喘模型大鼠肺组织PGD₂/EOS轴的影响[J].中医杂志,2026,67(20):2219-2226. DOI: 10.13288/j.11-2166/r.2026.20.012. DOI：

JIA Junjie,JIANG De,CHEN Yifei,et al.Effects of Different Degrees of "Dryness Pathogen" on the PGD₂/EOS Axis in the Lung Tissue of Bronchial Asthma Model Rats[J].Journal of Traditional Chinese Medicine,2026,67(20):2219-2226. DOI: 10.13288/j.11-2166/r.2026.20.012. DOI：

摘要

目的

从前列腺素D

（PGD

）/嗜酸性粒细胞（EOS）轴角度探讨“燥邪”对支气管哮喘模型大鼠的影响。

方法

将40只SD大鼠随机分为空白组、哮喘组、哮喘轻燥组、哮喘中燥组、哮喘重燥组，每组8只。哮喘组通过卵清蛋白（OVA）诱导建立哮喘模型，各哮喘燥证组通过气候箱联合OVA建立西北燥证哮喘动物模型。在造模期间观察并记录各组大鼠的一般行为学表现，比较体质量、饮水量、哮喘症状评分；在造模第21天，采用小动物呼吸系统检测大鼠呼吸频率和呼吸间歇收缩指数；采用HE染色法观察大鼠肺组织病理变化；采用吉姆萨染色观察肺组织嗜酸性粒细胞（EOS）的浸润情况，计算EOS数量；ELISA法检测血清前列腺素D

（PGD

）、白细胞介素-5（IL-5）、白细胞介素-4（IL-4）水平；Western Blot法检测大鼠肺组织中嗜酸性粒细胞过氧化物酶（EPX）和前列腺素D

受体（CRTH2）蛋白水平。

结果

与空白组比较，各哮喘燥证组平均饮水量升高（

＜0.01）；第14天哮喘中燥组、重燥组体质量降低，第21天各造模组大鼠体质量降低（

＜0.05或

＜0.01）；各造模组大鼠哮喘症状评分，呼吸频率，呼吸间歇收缩指数，肺组织EOS数量，血清PGD

、IL-5、IL-4水平，肺组织EPX、CRTH2蛋白相对表达量均升高（

＜0.01）。与哮喘组比较，各哮喘燥证组大鼠饮水量升高（

＜0.01）；第21天哮喘中燥组、重燥组大鼠体质量降低（

＜0.05）；各哮喘燥证组大鼠呼吸频率及血清中PGD

、IL-5、IL-4水平升高，肺组织EPX、CRTH2蛋白相对表达量亦升高（

＜0.05或

＜0.01）；中燥组、重燥组大鼠哮喘症状评分与呼吸间歇收缩指数升高，肺组织中EOS数量增加（

＜0.05或

＜0.01）。与哮喘轻燥组比较，哮喘重燥组大鼠血清PGD

、IL-5、IL-4水平与肺组织EPX、CRTH2蛋白相对表达量升高（

＜0.05或

＜0.01）。各哮喘燥证组大鼠肺部组织炎性浸润增多，肺泡壁增厚、管腔狭窄、肺间质充血、黏性分泌物分泌加重，且病理表现随干燥程度加重。

结论

“燥邪”可通过肺组织PGD

/EOS轴加重支气管哮喘症状及肺组织病理损伤，并且随着“燥邪”程度的增高逐步加重。

Abstract

Objective

To explore the effects of "dryness pathogen" on a bronchial asthma rat model from the perspective of the prostaglandin D

（PGD

）/eosinophil （EOS） axis.

Methods

Forty SD rats were randomly divided into five groups， blank group， asthma group， mild dryness asthma group， moderate dryness asthma group， and severe dryness asthma group， with 8 rats in each group. The asthma model was induced in the asthma group using ovalbumin （OVA）， while the asthma-dryness syndrome groups were modeled using a climate chamber combined with OVA to establish animal models of bronchial asthma with Northwest dryness syndrome. During modeling， general behavioral performance of the rats in each group was observed and recorded， and body weight， water intake， and asthma symptom scores were compared. On day 21 of the modeling， small animal respiratory system testing was used to measure respiratory rate and intermittent respiratory contraction index. Pathological changes in rat lung tissues were observed using HE staining. Giemsa staining was used to observe eosinophil （EOS） infiltration in lung tissues and calculate EOS counts. ELISA was used to detect serum levels of prostaglandin D

（PGD

）， interleukin-5 （IL-5）， and interleukin-4 （IL-4）. Western Blot was used to detect eosinophil peroxidase （EPX） and prostaglandin D

receptor （CRTH2） protein levels in rat lung tissues.

Results

Compared to the blank group， the average water intake of rats in all asthma-dryness groups increased （

＜0.01）. The body weight of rats decreased in the moderate and severe asthma-dryness groups on day 14， and in all modelling groups on day 21 （

＜0.05 or

＜0.01）. Across all model groups， the asthma symptom score， respiratory rate，

intermittent respiratory contraction index， EOS count in lung tissue， serum levels of PGD

， IL-5， and IL-4， as well as the relative protein expression levels of EPX and CRTH2 in lung tissue all significantly increased （

＜0.01）. Compared to the asthma group， the water intake of rats in the mild， moderate， and severe asthma-dryness groups increased （

＜0.01）. On day 21 of the experiment， the body weight of rats in the moderate and severe asthma-dryness groups decreased （

＜0.05）， and the respiratory rate of rats in all asthma-dryness groups increased， as well as the serum levels of PGD

， IL-5， and IL-4， and the relative protein expression levels of EPX and CRTH2 in lung tissues （

＜0.05 or

＜0.01）. Furthermore， in the moderate and severe asthma-dryness groups， the asthma symptom scores and intermittent respiratory contraction indices were elevated， and the EOS counts in lung tissue increased （

＜0.05 or

＜0.01）. Compared to the mild asthma-dryness group， serum levels of PGD

， IL-5， and IL-4 in the severe asthma-dryness group increased， as well as the relative protein expression levels of EPX and CRTH2 in lung tissue （

＜0.05 or

＜0.01）. Pathological observation showed that rats in all asthma-dryness syndrome groups exhibited increased inflammatory infiltration in the lung tissue， alveolar wall thickening， luminal narrowing， pulmonary interstitial congestion， and aggravated viscous secretions. These pathological manifestations worsened as the degree of dryness increased.

Conclusion

The "dryness pathogenic factor" can exacerbate bronchial asthma symptoms and lung tissue pathological damage through the PGD

/EOS axis， and the damage progressively worsens with the increasing severity of the dryness pathogen.

关键词

Keywords

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Effects of Different Degrees of "Dryness Pathogen" on the PGD2/EOS Axis in the Lung Tissue of Bronchial Asthma Model Rats

DOI：10.13288/j.11-2166/r.2026.20.012

摘要

Abstract

关键词

Keywords

references

Effects of Different Degrees of "Dryness Pathogen" on the PGD₂/EOS Axis in the Lung Tissue of Bronchial Asthma Model Rats