Mice with ascites U

14

carcinoma weretreated（ip.200mg/kg for once only）withthe extract of Spirobolus bungii Brandt.Anincrease in cancer cell death ratio of 35%was seen at 2 hr.after treatment.The in-jured cells presented karyopyknosis

karyocla-sis

cytoclasis and vacuolization.Tumor mi-tosis was immediately arrested.After up to24 hr.of exposure

the ratio of mitosis fellbelow the starting level.Scanning electronmicroscopy also revealed significant changes.The plasma membrane of cancer cell appea-red deepening of gap

increasing in smallfingerilike processes and rising of large gra-nules.The findings indicated that the ex-tract had direct destructive effect on cancercell.The activity happened at 1 hr.aftersingle treatment and lasted for 6 hours.