Preliminary Study on HL -60 Cellular Wither Induced by Chloroform Extract of Yisuiling[J]. Journal of Traditional Chinese Medicine1999, (4): 235-236.DOI: 10.13288/j.11-2166/r.1999.04.025.
The experiment was conducted on the actions of 5 kinds of reagents on HL - 60 cellular wither
viz. Yisuiling chloroform extract
rabbit serum chloroform extract
rabbit liver paste chloroform extract
Etoposide
and normal rabbit serum. Results revealed that after fluid incubation for a short period
the HL-60 cultured cells showed no wither in the former 4 groups under microscope and flowing cytometer. Among them
action was strongest in rabbit liver paste of Yisuiling extract group
similar to the positive control of Etoposide. Followed by rabbit serum
and the action was weak in Yisuiling chloroform extract. There was no obvious action of induced wither by normal rabbit serum. The mechanism of induced wither is still under investigation.