Objective: To observe the effect of heart deficiency syndrome on microstructure and ultra-microstructure of cardiac muscle cells. Methods: Healthy rats were randomly divided into model group and control group. For model group

cardiac muscle was taken respectively after 48 hours and 72 hours to make frozen slices and ultra-filmy slices. Fluorescence and electric microscope were used to observe changes of distribution

content and ultra-microstructure of hepatin and DNA within cardiac muscle cells. Results: After 48 hours

fluorescence PAS reaction of cardiac muscle cells of model group reduced significantly

and after 72 hours

electropositive matters in DAN fluorescence reaction was much less than that of control group. Based on tridimensional and quantificational analysis

there was significant difference in chondriosome density between two groups （P < 0. 01）. Conclusion: Syndrome of heart deficiency could result in injury of cardiac muscle cells and affect metablism of cardiac muscle and structure of cardiac muscle cells.