Effect of Yuzhang Decoction on In Vitro Proliferation of Bone Marrow CD34 Cells in the Patient of Aplastic Anemia[J]. Journal of Traditional Chinese Medicine2004, (6): 432-433. DOI: 10.13288/j.11-2166/r.2004.06.021.
Effect of Yuzhang Decoction on In Vitro Proliferation of Bone Marrow CD34 Cells in the Patient of Aplastic Anemia[J]. Journal of Traditional Chinese Medicine2004, (6): 432-433. DOI: 10.13288/j.11-2166/r.2004.06.021.DOI:
Objective: To observe effects of Yuzhang Decoction on in vitro proliferation of bone marrow CD
34
cells in the patient of aplastic anemia (CAA) and its mechanism. Methods: To adopt monoclone antibody-immune magnetic beads separation system (MACS) to separate and purify CD
34
cells in bone marrow of the patient of CAA
and make cell culture
applying
3
H-TdR incorporation to detect effect of Yuzhang Decoction on proliferation of CD
34
cells of bone marrow. Results: CD
34
cells of bone marrow showed concentration-dependent proliferation in the Yuzhang Decoction group with significantly differences (P
<
0. 05 or 0. 01). Conclusion: Yuzhang Decoction can improve in vitro proliferation of CD
34
cells of bone marrow of the patient of CAA. Yuzhang Decoction curing CAA is carried out possibly through promoting proliferation of CD