Objective:To investigate the effect of Chinese compound drug Changfukang on apoptosis of human large intestinal cancer cells and to explore its mechanism.Methods:Nude mouse model of transplanting tumor of human large intestinal cancer HT-29 was established.The low

medium and high dose groups of Changfukang

the blank control group and 5-FU plus CF group were set up.Eight weeks after medication

all mice were killed and the tumor weights were measured

the apoptotic indexes were tested with terminal deoxynucleotidy transferase-mediated dUTP nick end labeling

the tumor bodies were respectively stained by Caspase-3

Bax and Bcl-2 immunohistochemistry and their expressions were quantitatively measured and compared by an image analysis system.Results:The tumor weight in each Changfukang group was lower than that in the blank control group

but the apoptotic index was higher than that in the blank and 5-FU plus CF groups.The apoptotic index of the high dose group was significantly higher than that in the blank group and the 5-FU plus CF group（P<0.01）.The expression of Caspase-3 and the ratio of Bax and Bcl-2 expression（Bax/Bcl-2）in each Changfukang group was higher than that in the [)lank group.Conclusion: Changfukang has the function of inducing apoptosis of nude mouse transplantation tumor of human large intestinal cancer HT-29

and the mechanism is possibly related to Changfukang enhancing expression of Caspase-3 and amplifying the ratio of Bax and Bcl- 2 expression in transplanted tumor cells.