Zhang Jun Cheng Jianxin Shan Baoen. Effects of Shenkui Decoction on Proliferation of Ovarian Cancer Fresh Parenchymatous Tumor Cells and Ovarian Cancer Cell Line Tyk-nu Cells[J]. Journal of Traditional Chinese Medicine2005, (3): 223-225.
Zhang Jun Cheng Jianxin Shan Baoen. Effects of Shenkui Decoction on Proliferation of Ovarian Cancer Fresh Parenchymatous Tumor Cells and Ovarian Cancer Cell Line Tyk-nu Cells[J]. Journal of Traditional Chinese Medicine2005, (3): 223-225.DOI: 10.13288/j.11-2166/r.2005.03.034.
Objective:To observe effects of Shenkui Decoction on proliferation of ovarian cancer cell.Methods:The Shenkui Decoction- containing rat serum was prepared by using TCM serum pharmacological method
and effects of the drug containing serum on proliferation of ovarian cancer fresh parenchymatous tumor cells were investigated with 3H incorporation method
and effects of the drug-containing serum on proliferation of ovarian cancer cell line Tyk-nu cells were investigated by flow cytometry
cellular activity assay
cellular growth curve assay and cell colony formation rate assay.Results:The drug containing serum could inhibit proliferation of both fresh parenckymatous tumor cells and ovarian cancer cell line Tyk-nu cells
and the inhibitory rate raised with the increase of drug-containing serum content.Conclusion:The Shenkui Decoction-containing rat serum can inhibit proliferation of ovarian cancer fresh parenchymatous tumor cells and ovarian cancer cell line Tyk-nu cells.