Objective:To explore expression of apoptosis-related genes in the patient of blood deficiency and the mechanism of Digan Oral Liquid for treatment of blood deficiency.Methods:Expressions of marrow monocyte cyclin D1

apoptosis-related gene（bax/bcl-2） mRNA and apoptosis were detected with in situ hybridization in 30 cases of blood deficiency treated by Digan Oral Liquid and 10 healthy persons.Results:In the patient of blood deficiency

cell cycle was significantly inhibited

and the expressions of cyclin D1 mRNA and apoptosis-inhibiting gene bcl-2 mRNA decreased and the expression of apoptosis-promoting gene bax mRNA and the apoptosis rate increased.After treatment of Digan Oral Liquid

the expression of marrow monocyte cyclin D1 gene transcription increased

and differentiation and proliferation of cells were accelerated

so as to reduce apoptosis of marrow cells

decreasing the apoptosis rate.Conclusion:Genesis of blood deficiency may be related with cyclin and the apoptosis-related genes

and Digan Oral Liquid can decrease apoptosis of hematopoietic cells and accelerate differentiation and mature of cells

and make peripheral blood cells discontinuously renew

so as to cure blood deficiency.