Objective:To search for differentially expressed proteins in the liver tissue of the model rat with stagnation of the liver-qi by using technique and method of tissue proteomics.Methods:The rat model with stagnation of the liver-qi was established by restraint.Proteins in the liver tissue of the rat were extracted with histolysis step by step

and the attained proteins were separated by two-dimensional electrophoresis（2-DE）

with colloidal staining.The differential depressions of proteins were analyzed in the model group and the control group.The peptide mass fingerprints were detected with MALDI-TOF-MS

and the corresponding proteins were retrieved in Databank.Results:In the colloidal stained 2-DE map of the liver tissue of the rat

over 300 clear protein spots could be differentiated

and totally 3 differential expressed proteins in the liver tissue related with syndrome of stagnation of the liver-qi were found

the mass-spectrogram peaking rate being 100%.They were aryl sulfotransferase

enoyl-coa hydrase and transthyretin.Conclusion:The normal dispelling function of the liver is possibly related with differential expression of the above 3 transpotanted proteins.