Objective:To observe effects of electroacupuncture（EA） at The Conception Vessel on proliferation and differentiation of nerve stem cells in the subventricular zone of the lateral ventricle of cerebral ischemia rats.Methods:The middle cerebral artery was blocked for 2 hours and then reperfused.Model rats were randomly divided into two groups:control group and EA group.Changes of differentiation and proliferation of nerve stem cells were observed at 7

14

28 days after successfully modeling.Results:Compared with the control group

there was no significant difference（P>0.05） in the number of 5-bromodeoxyuridine（Brdu） positive cells

Brdu/GFAP

Brdu/Nestin and Brdu/Nse double-labeled cells in the subventricular zone in EA group 7 days after modeling.However

in the 14-day group and the 28-day group

the number of Brdu positive cells and Brdu/GFAP

Brdu/Nestin

Brdu/Nse double-labeled cells significantly increased as compared with the control group（P<0.05 or P<0.01）.Conclusion:EA at The Conception Vessel promotes differentiation and proliferation of nerve stem cells in the subventricular zone of the lateral ventricle of cerebral ischemia rats

and may promote proliferation of the nerve stem cell towards astrocytes.