Effect of Needling at Points of Pericardium Meridian of Hand Jueyin on the Expression of Protein Kinase C in Cardiocyte of Ische-mical Reperfusion Injury
TIAN Yue-feng1, WANG Rong1, LI Lei-yong2, et al. Effect of Needling at Points of Pericardium Meridian of Hand Jueyin on the Expression of Protein Kinase C in Cardiocyte of Ische-mical Reperfusion Injury[J]. Journal of Traditional Chinese Medicine2007, (8): 713-715.
TIAN Yue-feng1, WANG Rong1, LI Lei-yong2, et al. Effect of Needling at Points of Pericardium Meridian of Hand Jueyin on the Expression of Protein Kinase C in Cardiocyte of Ische-mical Reperfusion Injury[J]. Journal of Traditional Chinese Medicine2007, (8): 713-715.DOI: 10.13288/j.11-2166/r.2007.08.014.
Objective To study the effect of needling at points of Pericardium Meridian of Hand Jueyin on the expression of protein kinase C (PKC) and signal transduced mechanism mediated by PKC in cardiocyte of ischemical reperfusion injury. Methods Points of Pericardium Meridian of Hand Jueyin of rats were given electro-acupuncture for 20 minutes.Ramus descendens anterior arteriae coronariae sinistrae was ligated for 40 minutes with electrocardiogram monitoring.Ligation ceased after another 20 minutes of electro-acupuncture.Heart was extracted after 60 minutes of reperfusion.The expression level of PKC was tested by immunohistochemical method. Results The expression level of PKC increased in model group but decreased significantly after acupuncture (P<0.01). Conclusion Needling points of Pericardium Meridian of Hand Jueyin can obviously inhibit the expression level of PKC and protect cardiocyte.