YANG Bo-hua, ZHANG Juan-zi, ZHU Ling-qun, et al. Effect of Sanqi and Huangqi on the Differentiation in Vitro of Stem Cells in the Patients with Ischemia of Lower Limbs[J]. Journal of Traditional Chinese Medicine2007, (11): 994-995.
YANG Bo-hua, ZHANG Juan-zi, ZHU Ling-qun, et al. Effect of Sanqi and Huangqi on the Differentiation in Vitro of Stem Cells in the Patients with Ischemia of Lower Limbs[J]. Journal of Traditional Chinese Medicine2007, (11): 994-995.DOI: 10.13288/j.11-2166/r.2007.11.041.
Objective To explore the effect of herbal medicines Sanqi (Radix Notoginseng) and Huangqi (Radix Hedysari) on the differentiation in vitro of stem cells.Methods The marrow blood of the patient with ischemia of lower limbs was collected routinely. The density gradient centrifugation was adopted to separate the single nuclear cells
and the cells were amplified by under different concentration. Three weeks later
the morphological changes were observed. The flow cytometer (FCM) was used to test the percentage of CD34+ cells.Results The cells was shaped in fusiform and arranged in bunchy order with few circular cells mixed. The percentage of CD34+ cells was significantly increased in the Huangqi medium dosage group and Huangqi small dosage group and Sanqi medium dosage group
Sanqi large dosage group(P<0.05 or P<0.01).Conclusion Huangqi and Sanqi have the function of promoting the transformation and proliferation of stem cell to vascular endothelia precursor cells (EPC).