Objective To study the effect of Stasis-removing and muscle-regeneration therapy on the expression of Bax and Bcl-2 mRNA in granulation tissue of diabetic rats wound healing. Methods Totally 48 male SD diabetic rats with full-thickness skin lesion were randomized into 4 groups: Shengji Huayu （Muscle-regeneration and stasis-removing） Recipe-treated group 1

2

3 and normal saline control group. The tissues for test were collected on the 3rd and 11th day of the wound healing and the apoptosis-related gene Bax and Bcl-2 mRNA in granulation tissue were tested with real-time PCR method. Results On the 3rd day of wound healing

Bcl-2 mRNA copies and Bax/Bcl-2 mRNA of the rats in Shengji Huayu Recipe-treated group 1 and 2 were higher than those in the control group （P<0.05）. On the 11th day of wound healing

Bcl-2 mRNA copies of the rats in Shengji Huayu Recipe-treated group 1 was still higher than that in the control group （P<0.05）

but Bax/Bcl-2 mRNA of Shengji Huayu Recipe-treated group 2 was not significantly different from that of control group （P>0.05）. Conclusion Stasis-removing and muscle-regeneration herbs may promote wound healing in diabetic rats through regulating Bax and Bcl-2 mRNA expression in granulation tissue. At the early stage

using more stasis-removing herbs can inhibit the apoptosis

benefiting the wound healing

while using more muscle-regeneration herbs can promote the apoptosis at the late stage

benefiting the reduction of scar formation.