海昆肾喜对阿霉素肾病大鼠肾组织内皮素1及其mRNA表达和肾小球系膜细胞增殖的影响

刘昆; 赵宗江; 张新雪; 杨美娟

doi:10.13288/j.11-2166/r.2008.05.046

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海昆肾喜对阿霉素肾病大鼠肾组织内皮素1及其mRNA表达和肾小球系膜细胞增殖的影响

方药研究 | 更新时间：2021-11-17

- 海昆肾喜对阿霉素肾病大鼠肾组织内皮素1及其mRNA表达和肾小球系膜细胞增殖的影响
- Effect of Haikun Shenxi Capsule on Endothelin-1 and mRNA Expression in Kidney Tissue of Rats with Adriamycin-induced Nephrosis and Proliferation of Glomerular Mesanglal Cells
- 中医杂志 2008年第5期页码：456-458
- 作者机构：
  
  1. 北京中医药大学细胞与生化实验室
  2. 北京中医药大学细胞与生化实验室北京市朝阳区北三环东路11号
  3. ,100029
- 作者简介：
- 基金信息：
  
  教育部“长江学者和创新团队发展计划”资助项目（IRT0413）;国家自然科学基金重点（30130220）;高等学校学科创新引智计划资助项目（B07007）;北京市中医管理局“51510”资助项目（JJ2007-006）
- DOI：10.13288/j.11-2166/r.2008.05.046
  中图分类号： R285
- 纸质出版：2008
- 稿件说明：
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[1]刘昆,赵宗江,张新雪,杨美娟.海昆肾喜对阿霉素肾病大鼠肾组织内皮素1及其mRNA表达和肾小球系膜细胞增殖的影响[J].中医杂志,2008(05):456-458.

LIU Kun ZHAO Zong-jiang ZHANG Xin-xue. Effect of Haikun Shenxi Capsule on Endothelin-1 and mRNA Expression in Kidney Tissue of Rats with Adriamycin-induced Nephrosis and Proliferation of Glomerular Mesanglal Cells[J]. Journal of Traditional Chinese Medicine2008, (5): 456-458.
[1]刘昆,赵宗江,张新雪,杨美娟.海昆肾喜对阿霉素肾病大鼠肾组织内皮素1及其mRNA表达和肾小球系膜细胞增殖的影响[J].中医杂志,2008(05):456-458. DOI： 10.13288/j.11-2166/r.2008.05.046.

LIU Kun ZHAO Zong-jiang ZHANG Xin-xue. Effect of Haikun Shenxi Capsule on Endothelin-1 and mRNA Expression in Kidney Tissue of Rats with Adriamycin-induced Nephrosis and Proliferation of Glomerular Mesanglal Cells[J]. Journal of Traditional Chinese Medicine2008, (5): 456-458. DOI： 10.13288/j.11-2166/r.2008.05.046.

摘要

目的探讨海昆肾喜对阿霉素肾病大鼠内皮素1及其mRNA表达的影响以及对体外培养肾小球系膜细胞增殖的影响。方法将大鼠随机分为6组、正常组、假手术组、模型组、苯那普利组、海昆肾小剂量组及海昆肾喜大剂量组

10周后采用免疫组织化学及原位杂交方法分析肾组织内皮素1蛋白及mRNA表达情况;以苯那普利为实验对照组

采用MTT比色法测定海昆肾喜对肾小球系膜细胞增殖的影响。结果模型组大鼠肾组织中内皮素1及mRNA表达强阳性;海昆肾喜大、小剂量组较模型组表达显著减少（P<0.05或P<0.01）;并且海昆肾喜可以明显抑制肾小球系膜细胞的增殖。结论海昆肾喜能够减少阿霉素肾病大鼠肾组织中内皮素1及mRNA的表达

抑制肾小球系膜细胞的增殖

从而延缓肾衰竭的的病程进展。

Abstract

Objective To study the effect of Haikun Shenxi Capsule（HSC）on endothelin-1（ET-1）and mRNA expression of rats with adriamyein-induced nephrosis and proliferation of glomerular mesangial cells（GMCs）cultured in vitro.Methods The rats were randomized into 6 groups

normal group

sham operation group

model group

benazepril group

HSC low-and high-dose groups.Ten weeks after treatment

ET-1 and mRNA expression were measured with immunohistochemical and hybridization in situ method.Tak- ing the Benazepril group as the control group

the proliferation of GMCs was detected with MTT method.Results In the model group

ET-1 and mRNA expression presented a strong positive

compared with which

that of the HSC low-and high-dose groups was remarkably reduced（P<0.05）

and the effect of high-dose group was better as the proliferation of GMCs was remarkably suppressed. Conclusion HSC reduces remarkably ET-1 and mRNA expression in kidney tissue of rats with adriamycin-indueed nephrosis and de- creases the proliferation of GMCs

slowing down the development of renal failure.

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