Objective To study the functional mechanism of Yougui Pill（Right Restoring Pill）through its effect on cerebral gene of mice models with kidney-yang asthenia caused by excessive physical and sexual activity.Methods There were 10

15

10 male mice in the control

model and treatment groups respectively.The control and model groups were treated with 0.5ml permouse perday distilled water by gavage while the treatment group was given 0.5ml permouse perday suspension of Yougui Pill.In the model and treatment groups

the ratio of male and female was 1∶6

feeding in the same cage

and the males were swimming everyday for 30

4

0 minutes

4 weeks in succession

to induce the kidney-yang asthenia.The 36K mouse cerebral gene chips were used to measure the brain genes of mice of 3 groups.The intensity ratio of fluorescence signals ≥2 and ≤0.5 were used as the scale to screen out the remarkably different genes.Results In the model and control groups

totally 186 gene with different expressions were screened out

including the 150 up-regulated genes and 36 down-regulated genes.In the treatment and model groups

155 gene with different expressions were screened out

including the up-regulated 64 genes and 91 down-regulated genes.There were 32 genes up-regulated in the model and control groups but down-regulated in the treatment and model groups

mainly involving the inflammation perimmunity and neurotransmissions persignal transduction genes.There were 12 genes down-regulated in the model and control groups but up-regulated in the treatment and model group

mainly involving the hormone related genes of melanin and orphan nuclear receptor.Conclusion Yougui Pill may make the notably down-regulated genes of melanin markedly up-regulated in mice models of kidney-yang asthenia

improving the effect of hormone related orphan nuclear receptor and promoting the cellular proliferation.