Objective To observe the effect of Fufang Puhuang Granule（Compound Pollen Typhae Granule） on the expression of Heat Shock Protein-70（HSP-70） and its mRNA in rats with cerebral ischemia-reperfusion.Methods The 75 SD male rats were randomized into 5 groups（n=15）: sham operation group

ischemia reperfusion model group

Puhuang Granule large

medium

and small does groups.The rats were surgically prepared for occlusion of the middle cerebral artery.Reperfusion was conducted at 2 hours after ischemia.At 6

24

72 hours after reperfusion the rats were decapitated

5 at each time point.At 24 hours after reperfusion

scores of neurological deficit were estimated

the morphologic changes of neurons were observed by using Nissl staining

the expression of HSP-70 mRNA were examined by using in situ hybridization

and the expression of HSP-70 was detected by immunohistochemical method.Results The effect of Puhuang Granule large and medium groups at 24 hours after reperfusion on improving the neurological functions was better than that of the model group（P

<

0.05）.The result of Nissl staining showed irregular shaped and small sized neurons with pyknosis

interstitial edema

and Nissel body decrease in the model group

and there was an obvious neuron loss.But in all Puhuang Granule groups

there was no obvious injured neurons and neuron loss.In the model group

the expression of HSP-70 and its mRNA were both enhanced at 6

7

2 hours after reperfusion

compared with the sham operation group at each time point

the difference was significant（P

<

0.01）.The expression of HSP-70 and its mRNA were enhanced in all Puhuang Granule groups

compared with the sham operation group at each time point

the difference was significant（P

<

0.05）.Conclusion Puhuang Granule improves the expression of HSP-70 of rats with the injury of cerebral ischemia-reperfusion.