黄芪、水蛭及其配方含药血清对大鼠系膜细胞生长周期及凋亡的影响

任现志; 蒋淑敏; 翟文生

doi:10.13288/j.11-2166/r.2009.03.044

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黄芪、水蛭及其配方含药血清对大鼠系膜细胞生长周期及凋亡的影响

方药研究 | 更新时间：2021-11-17

- 黄芪、水蛭及其配方含药血清对大鼠系膜细胞生长周期及凋亡的影响
- Effect of the Serum Contained Radix Astragali and Hirudo and Their Compound on the Growth Cycle and Apoptosis of Rat Glomerular Mesangial Cells
- 中医杂志 2009年50卷第3期页码：255-257
- 作者机构：
  
  1. 南京中医药大学博士后流动站
  2. 中国药科大学
  3. 河南中医学院第一附属医院
- 作者简介：
- 基金信息：
  
  江苏省高校自然科学研究计划项目（03KJB360095）
- DOI：10.13288/j.11-2166/r.2009.03.044
  中图分类号： R285.5
- 纸质出版：2009
- 稿件说明：
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[1]任现志,蒋淑敏,翟文生.黄芪、水蛭及其配方含药血清对大鼠系膜细胞生长周期及凋亡的影响[J].中医杂志,2009,50(03):255-257.

REN Xianzhi1, JIANG Shumin2, ZHAI Wensheng3. Effect of the Serum Contained Radix Astragali and Hirudo and Their Compound on the Growth Cycle and Apoptosis of Rat Glomerular Mesangial Cells[J]. Journal of Traditional Chinese Medicine2009, 50(3): 255-257.
[1]任现志,蒋淑敏,翟文生.黄芪、水蛭及其配方含药血清对大鼠系膜细胞生长周期及凋亡的影响[J].中医杂志,2009,50(03):255-257. DOI： 10.13288/j.11-2166/r.2009.03.044.

REN Xianzhi1, JIANG Shumin2, ZHAI Wensheng3. Effect of the Serum Contained Radix Astragali and Hirudo and Their Compound on the Growth Cycle and Apoptosis of Rat Glomerular Mesangial Cells[J]. Journal of Traditional Chinese Medicine2009, 50(3): 255-257. DOI： 10.13288/j.11-2166/r.2009.03.044.

摘要

目的比较黄芪、水蛭及其配方含药血清对大鼠系膜细胞生长周期、凋亡的影响。方法制备水蛭高浓度（水蛭组）、黄芪高浓度（黄芪组）和水蛭高黄芪低浓度（配方组）含药血清

并对大鼠系膜细胞（MC）进行干预培养

应用流式细胞仪检测MC生长周期与凋亡

显微镜观察凋亡细胞的形态。结果各组含药血清均能使大量MC处于G0/G1期

与对照组比较差异有统计学意义（P<0.01）;各组亦均能提高大鼠MC凋亡率

水蛭组、配方组与对照组比较差异有统计学意义（P<0.01）。结论水蛭、黄芪及其配方含药血清均能阻止MC进入S期从而达到抑制增生的目的

且能提高MC的凋亡率。

Abstract

Objective To study the effect of the serum contained Radix Astragali

Hirudo or their compound on the growth cycle and apoptosis of rat mesangial cells （MC）.Methods The serum contained the extract of high-concentration of Hirudo

the serum contained the extract of high-concentration of Radix Astragali

and the serum contained the extract of high-concentration of Hirudo and the extract of low-concentration of Radix Astragali were prepared. The cultured MC were measured by flow cytometer to observe the changes in their growth cycle and apoptosis. The morphological changes of apoptotic cells were observed.Results The medicinal serums can keep the most MC in the stage of G0/G1

and the significant difference was existing in comparison with that of the control group （P<0.01）. The apoptosis rate of MC in the medicinal groups was increased

and the difference was shown in the Hirudo and compound groups in comparison with that of the control group （P<0.01）.Conclusion The medicinal serums contained Hirudo and Radix Astragali and their compound respectively can prevent MC entering the stage of S to inhibit their growth

and they can increase the rate of apoptosis of MC as well.

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