Objective To study the effect of time-choosing administration of spleen-reinforcing therapy on the blood glucose level and its circadian rhythm of experimental diabetic mice.Methods Alloxan 200mg/kg was injected to establish the model of diabetes mellitus.All diabetic mice were randomly divided into three groups

and then given by gastric perfusion of Jianpi Recipe（Recipe to reinforce the spleen） at 6 am

6 pm and normal saline respectively.The normal control group was set up for contrast.Bloodglucose concentration was examined every fourhours in all groups including normal mice at the day of pre-treatment

at the day after one-week treatment and at the day after two-week treatment.Bloodglucose-time diagrams were depicted respectively.All biological time-series of bloodglucose concentrations were analyzed with macroscopic analysis.Results The peak and valley of bloodglucose concentrations were alternatively presented in the normal mice.The circadian rhythm of normal bloodglucose concentrations was obvious and its peak concentrations presented in 10am and 10pm

its valley concentrations presented in 6 am and 6 pm.The normal bloodglucose concentrations were always under 10.0mmol/L.The bloodglucose concentrations of diabetic mice were obviously above 10.0mml/L

and its circadian rhythm was obviously abnormal compared with the normal mice.The peak concentrations of the diabetic mice were presented in 10am and 2am

and its valley concentrations presented in 2 pm and 6 am.There is not significant difference between the peak blood glucose and valley value in diabetic mice（P>0.05）.The two-week administration of Jianpi Recipe decreased the bloodglucose of diabetic mice.Normal saline had no obvious influence on it.Time-choosing recipe administration could adjust the circadian rhythm of bloodglucose concentration.Administration at 6am was more effective in adjusting bloodglucose rhythm.Conclusion Spleen-reinforcing therapy can decrease the bloodglucose of diabetic mice

and the effect tend to be more obvious with the administration prolonging.Administration at different time exerts different effect on circadian rhythm of bloodglucose concentrations.There exists different effect in different time administration in spleen-reinforcing therapy.