Objective To investigate the mechanism of Bushen Huoxue Recipe （Recipe to reinforce the kidney and activate blood） in the treatment of glomerulosclerosis of the rats with mesangial proliferative glomerulonephritis （MsPGN）.Methods Fifty-four SD male rats were randomized into normal group

model group

and treated group with 18 rats in each. The MsPGN models were made by injection of anti-rat-thymocyte antiserum （ATS） through the tail vein. After modeling

the treated group was administered Bushen Huoxue Recipe with a dosage of 5ml/kg

once a day. Six rats from each group were selected in the second

fourth

and eighth week respectively to determine the content of urine protein （Upro）

blood urea nitrogen （BUN）

and serum creatinine （SCr）

and the kidney was cut for pathological observation and immunohistochemical detection of the kidney tissues

the expression of Ⅳ-type collagen （Col-Ⅳ） and laminin （LN）.Results The pathological determination showed that

at the same time points

the hyperplasia of mesangial cells in the treated group was significantly relieved and mesangial matrix reduced as compared with the model group. The content of Upro

BUN and SCr at all time points in the treated group was significantly decreased as compared with the model group （P<0.05 or P<0.01）. In the eighth week

the content of LN and Col-Ⅳ of the kidney tissue and the value of integral optical density （ILD） in the treated group were both significantly lowered as compared with the model group （P<0.05）.Conclusion Bushen Huoxue Recipe inhibits the expression of Ⅳ-type Collagen and Laminin of the MsPGN rats

being the possible mechanism in the treatment of glomerulosclerosis.