Objective To investigate the effect and mechanism of Artemisia Argyi extracting solution on anti-hepatic fibrosis.Methods Twenty-four experimental Wistar rats were randomized into four groups:Artemisia group

Danshen group

physiological saline group

and blank control group

with 6 in each.The Artemisia group was treated with 20% Artemisia Argyi extracting solution

the Danshen group with Danshen （salvia） solution

and the physiological saline group with physiological saline by gavage

20ml/kg.After treating three times per day for 3 days

sera were obtained and made a mixture with DMEM to be volume fraction as 5%

10%

and 20%.Effect of different volume fraction of extracting solution on TGF-β1 Smad3 and Smad7 mRNA and protein were detected.Results After treating with 5%

10% and 20% Artemisia Argyi extracting solution contained rat serum for 24h

contrasted to the black control group

the expression of TGF-β1 mRNA level was （0.82±0.03）

（0.72±0.03） and （0.67±0.04） times

and the expression of TGF-β1 protein was （0.66±0.09）

（0.34±0.05） and （0.31±0.07） times respectively;contrasted to the physiological saline group

the expressions of TGF-β1 mRNA and protein were significantly decreased （P<0.05）

and the expressions of Smad3 mRNA and protein were down-regulated and at the same time the expressions of Smad7 mRNA and protein were up-regulated.There was no significant difference between the 20% Artemisia Argyi extracting solution contained rat serum group and the Danshen group.Conclusion Artemisia Argyi extracting solution contained rat serum plays a role in anti-fibrosis by the way of reducing TGF-β1 and Smad3 and up-regulating Smad7

which leads to inhibiting the activation and proliferation of HSC-T6 to reduce the collagen expression and secretion.